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Optimize - Reagents application n Crystallization grade reagents for formulating screens or for optimization features n Quantitive formulation n Lot to lot consistency n Synergistic with Hampton Research screens & kits n Quality and convenience n Sterile filtered description Optimize reagents are quantitatively formulated macromolecular crystallization grade reagents designed specifically for the crystallization of proteins, peptides, and nucleic acids. Each Optimize solution is formulated using high purity salts, polymers, and buffers. Sterile filtered Optimize reagents are formulated at convenient, ready to use concentrations. Optimize reagents remove the guesswork and make the process of reproducing preliminary screening conditions and general optimization faster, easier, and more convenient. When using Optimize reagents, a scientist moves directly from the screen to the optimization with no time wasted searching for and formulating salts, buffers, and viscous polymers. Each Optimize reagent is supplied with a Certificate of Analysis as documentation of reagent quality and performance. Optimize buffer reagents are supplied with calibrated titration curves to make titration easy, fast, and accurate. optimize crystallization grade reagents Polymers Ethylene imine polymer HR2-599 50% solution 200 ml $78.00 Synonyms: PEI or Poly(ethylene imine) or Poly(ethyleneimine) solution or Ethyleneimine polymer solution Mr 600,000 - 1,000,000 CAS [9002-98-6] EC No 205-793-9 Density: 1.08 g/mL at 20°C Measured pH Range: 12.0 - 12.1 at 25°C Refractive Index n20/D: 1.450 Conductivity Range: 244.3 - 257.0 mS at 25°C Jeffamine ® ED-2001 pH 7.0 HR2-597 50% w/v solution pH 7.0 200 ml $98.00 Synonyms: O,O'-Bis(2-aminopropyl) polypropylene glycol-block-polyethylene glycol-block-polypropylene glycol 1,900 CH 3 CH(NH 2 )CH 2 (OCH 2 CH 2 ) n OCH 2 CH(NH 2 )CH 3 Mr ~2,000 CAS [65605-36-9] Refractive Index Range: 1.40637 - 1.40666 at 20°C Conductivity Range: 4.0 - 5.7 mS at 25°C pH before titration with Hydrochloric acid is > 10 Final measured pH of HR2-597 is 7.0 Jeffamine ® M-600 ® HR2-501 50% v/v solution pH 7.0 200 ml $94.00 HR2-503 100% solution (untitrated pH >12) 200 ml $94.00 Synonyms: O-(2-Aminopropyl)-O'-(2-methoxyethyl)polypropylene glycol 500 or Polypropylene glycol 500 mono-2-aminoethyl mono-2-methoxyethyl ether CH 3 OCH 2 CH 2 O[CH(CH 3 )CH 2 O] n CH 2 CH(NH 2 ) Mr 600 CAS [77110-54-4] Density: 0.983 g/mL at 20°C HR2-501: Refractive Index Range: 1.40754 - 1.40988 at 20°C Conductivity Range: 8.8 - 10.3 mS at 25°C HR2-503: Refractive Index Range: 1.44501 - 1.44546 at 20°C Conductivity Range: 0.0 - 0.1 mS at 25°C Pentaerythritol ethoxylate (3/4 EO/OH) HR2-743 40% v/v solution 200 ml $46.00 Synonyms: Pentaerythritol ethoxylate (15/4 EO/OH) C(CH 2 (OCH 2 CH 2 ) n OH) 2 Average Mn ~270 CAS [30599-15-6] EC No 500-071-2 Density: 1.2 g/mL at 25°C (lit.) Measured pH Range: 7.5 - 8.7 at 25°C Refractive Index: 1.39719 at 20°C Conductivity Range: 37.8 - 40.5 mS at 25°C H 2NCHCH 2 (OCHCH 2) a (OCH 2CH 2) b (OCH 2CH) c NH 2 CH 3 CH 3 CH 3 b 38.7, (a+c) 6.0 H 2N NH ( O ( ( 2 O x y z CH 3 CH 3 CH 3 O ( y 39, (x+z) 6 ( ( Pentaerythritol ethoxylate (15/4 EO/OH) HR2-745 50% v/v solution 200 ml $54.00 Synonyms: Pentaerythritol ethoxylate (3/4 EO/OH) C(CH 2 (OCH 2 CH 2 ) n OH) 4 , N ~3.75 Average Mn ~797 CAS [30599-15-6] EC No 500-071-2 Density: 1.2 g/mL at 25°C (lit.) Measured pH Range: 5.2 - 6.0 at 25°C Refractive Index Range: 1.41401 - 1.41578 at 20°C Conductivity Range: 4.2 - 11.4 mS at 25°C Pentaerythritol propoxylate (5/4 PO/OH) HR2-739 50% v/v solution 200 ml $54.00 Synonyms: Pentaerythritol propoxylate C[CH 2 [OCH 2 CH(CH 3 )] n OH] 4 , N ~5 Average Mn ~426 CAS [9051-49-4] EC No 500-030-9 Density of 100% solution: 1.05 g/mL at 25°C Measured pH Range: 7.7 - 8.3 at 25°C Refractive Index: 1.40424 - 1.40433 at 20°C Conductivity Range: 3.8 - 4.7 mS at 25°C Pentaerythritol propoxylate (17/8 PO/OH) HR2-741 50% v/v solution 200 ml $58.00 Synonyms: Pentaerythritol propoxylate C(CH 2 (OCH 2 CH(CH 3 )) n OH) 4 , N = 2.1 Average Mn ~629 CAS [9051-49-4] EC No 500-030-9 Density: 1.05 g/mL at 25°C (lit.) Measured pH Range: 4.3 - 4.6 at 25°C Refractive Index: 1.40418 at 20°C Conductivity Range: 6.1 - 6.9 mS at 25°C Poly(acrylic acid sodium salt) 5,100 HR2-773 50% w/v solution 200 ml $95.00 Synonyms: Poly(sodium acrylate) or Sodium polyacrylate C 3 H 3 NaO 2 Mr 94.04 CAS [9003-04-7] Measured pH Range: 6.4 - 6.9 at 25°C Refractive Index Range: 1.40716 - 1.40821 at 20°C Conductivity Range: 30.5 - 41.1 mS at 25°C Polyethylene glycol 200 HR2-601 100% solution 200 ml $30.00 Synonyms: PEG 200 H(OCH 2 CH 2 ) n OH M r 190 - 210 CAS [25322-68-3] EC No 500-038-2 Density: 1.124 g/mL at 20°C Measured pH Range: 8.0 - 10.2 at 25°C Refractive Index Range: 1.46008 - 1.46083 at 20°C Conductivity Range: 2.5 - 2.8 mS at 25°C 40 Hampton Research • Phone: 800-452-3899 or 949-425-1321 • Fax: 949-425-1611 • www.hamptonresearch.com • Customer Service: info@hrmail.com • Technical Support: tech@hrmail.com
Polymers Polyethylene glycol 300 HR2-517 100% solution 200 ml $45.00 Synonyms: PEG 300 H(OCH 2 CH 2 ) n OH M r 285 - 315 CAS [25322-68-3] EC No 500-038-2 Density: 1.125 g/mL at 20°C Measured pH Range: 8.0 - 8.5 at 25°C Refractive Index Range: 1.46492 - 1.46535 at 20°C Conductivity Range: 7.7 - 8.1 mS at 25°C Peroxides (as H 2 O 2 ).≤ 0.001% Al............................≤ 0.0005% As.........................≤ 0.00001% Ba...........................≤ 0.0005% Bi............................≤ 0.0005% Ca.............................≤ 0.001% Cd...........................≤ 0.0005% Cl..............................≤ 0.005% Polyethylene glycol 400 HR2-603 100% solution 200 ml $53.00 Synonyms: PEG 400 H(OCH 2 CH 2 ) n OH M r 380 - 420 CAS [25322-68-3] EC No 500-038-2 Density: 1.126 g/mL at 20°C Measured pH Range: 9.1 - 9.7 at 25°C Refractive Index Range: 1.46686 - 1.46761 at 20°C Conductivity Range: 2.5 - 2.8 mS at 25°C Polyethylene glycol 1,000 HR2-523 50% w/v solution 200 ml $90.00 Synonyms: PEG 1,000 H(OCH 2 CH 2 ) n OH M r 950 - 1050 CAS [25322-68-3] EC No 500-038-2 Measured pH Range: 6.5 - 7.7 at 25°C Refractive Index Range: 1.40078 - 1.40104 at 20°C Conductivity Range: 23.1 - 37.5 mS at 25°C Peroxides (as H 2 O 2 ).≤ 0.001% Al............................≤ 0.0005% As.........................≤ 0.00001% Ba...........................≤ 0.0005% Bi............................≤ 0.0005% Ca.............................≤ 0.001% Cd...........................≤ 0.0005% Cl..............................≤ 0.005% Co...........................≤ 0.0005% Cr...........................≤ 0.0005% Cu...........................≤ 0.0005% Fe...........................≤ 0.0005% K.................................≤ 0.02% Li............................≤ 0.0005% Mg..........................≤ 0.0005% Mn..........................≤ 0.0005% Mo..........................≤ 0.0005% Na...............................≤ 0.02% Ni............................≤ 0.0005% Pb...........................≤ 0.0005% SO 4 ..........................≤ 0.005% Sr............................≤ 0.0005% Zn...........................≤ 0.0005% Polyethylene glycol 1,500 HR2-525 50% w/v solution 200 ml $60.00 Synonyms: PEG 1,500 H(OCH 2 CH 2 ) n OH M r 1,400 - 1,600 CAS [25322-68-3] EC No 500-038-2 Measured pH Range: 6.1 - 8.7 at 25°C Refractive Index Range: 1.40117 - 1.40127 at 20°C Conductivity Range: 39.7 - 48.5 mS at 25°C Polyethylene glycol 3,350 Monodisperse HR2-591 flake 500 g $118.00 HR2-527 50% w/v solution 200 ml $80.00 HR2-837 25% w/v PEG 3,350, 0.1 M MES pH 5.8 100 ml $95.00 HR2-841 25% w/v PEG 3,350, 0.1 M HEPES Na pH 6.8 100 ml $95.00 HR2-845 25% w/v PEG 3,350, 0.1 M BIS-TRIS pH 7.8 100 ml $95.00 HR2-849 25% w/v PEG 3,350, 0.1 M BIS-TRIS propane pH 7.8 100 ml $95.00 Synonyms: PEG 3,350 H(OCH 2 CH 2 ) n OH M r 3,300 - 3,400 CAS [25322-68-3] EC No 500-038-2 HR2-527: Measured pH Range: 7.9 - 9.7 at 25°C Refractive Index Range: 1.40164 - 1.40208 at 20°C Conductivity Range: 44.0 - 56.5 mS at 25°C HR2-837: Measured Conductivity: 826 mS at 25°C HR2-841: Measured Refractive Index: 1.37193 at 20°C Measured Conductivity: 4.4 mS at 25°C HR2-845: Measured Conductivity: 379 mS at 25°C HR2-849: Measured Conductivity: 3.7 mS at 25°C Co...........................≤ 0.0005% Cr...........................≤ 0.0005% Cu...........................≤ 0.0005% Fe...........................≤ 0.0005% K...................................≤ 0.2% Li............................≤ 0.0005% Mg..........................≤ 0.0005% Mn..........................≤ 0.0005% Mo..........................≤ 0.0005% Na.................................≤ 0.2% Ni............................≤ 0.0005% Pb...........................≤ 0.0005% SO 4 ..........................≤ 0.005% Sr............................≤ 0.0005% Zn...........................≤ 0.0005% Polyethylene glycol 4,000 HR2-605 flake 500 g $80.00 HR2-529 50% w/v solution 200 ml $80.00 Synonyms: PEG 4,000 H(OCH 2 CH 2 ) n OH M r 3,500 - 4,500 CAS [25322-68-3] EC No 500-038-2 HR2-529: Measured pH Range: 6.9 - 9.5 at 25°C Refractive Index Range: 1.40198 - 1.40279 at 20°C Conductivity Range: 38.6 - 46.9 mS at 25°C Polyethylene glycol 6,000 HR2-513 flake 500 g $122.00 HR2-533 50% w/v solution 200 ml $80.00 Synonyms: PEG 6,000 H(OCH 2 CH 2 ) n OH M r 5,000 - 7,000 CAS [25322-68-3] EC No 500-038-2 HR2-533: Measured pH Range: 6.2 - 9.1 at 25°C Refractive Index Range: 1.40209 - 1.40249 at 20°C Conductivity Range: 32.5 - 39.6 mS at 25°C DNases.............. none detected RNases.............. none detected Proteases........... none detected Phosphatases.... none detected Peroxides (H 2 O 2 )......≤ 0.001% Al.............................≤ 0.0005% As..........................≤ 0.00001% Ba............................≤ 0.0005% Bi.............................≤ 0.0005% Ca..............................≤ 0.001% Cd............................≤ 0.0005% Cl...............................≤ 0.005% Co............................≤ 0.0005% Cr............................≤ 0.0005% Cu............................≤ 0.0005% Fe............................≤ 0.0005% K..................................≤ 0.02% Li.............................≤ 0.0005% Mg...........................≤ 0.0005% Mn...........................≤ 0.0005% Mo...........................≤ 0.0005% Na................................≤ 0.02% Ni.............................≤ 0.0005% Pb............................≤ 0.0005% SO 4 ...........................≤ 0.005% Sr.............................≤ 0.0005% Zn............................≤ 0.0005% Polyethylene glycol 8,000 HR2-515 flake 500 g $122.00 HR2-535 50% w/v solution 200 ml $80.00 Synonyms: PEG 8,000 H(OCH 2 CH 2 ) n OH M r 7,000 - 9,000 CAS [25322-68-3] EC No 500-038-2 HR2-535: Measured pH Range: 6.6 - 9.5 at 25°C Refractive Index Range: 1.40206 - 1.40261 at 20°C Conductivity Range: 30.4 - 39.3 mS at 25°C DNases............. none detected RNases............. none detected Proteases.......... none detected Phosphatases... none detected Peroxides (as H 2 O 2 ).≤ 0.001% Al............................≤ 0.0005% As.........................≤ 0.00001% Ba...........................≤ 0.0005% Bi............................≤ 0.0005% Ca.............................≤ 0.001% Cd...........................≤ 0.0005% Cl..............................≤ 0.005% Co...........................≤ 0.0005% Cr...........................≤ 0.0005% Cu...........................≤ 0.0005% Fe...........................≤ 0.0005% K.................................≤ 0.02% Li............................≤ 0.0005% Mg..........................≤ 0.0005% Mn..........................≤ 0.0005% Mo..........................≤ 0.0005% Na...............................≤ 0.02% Ni............................≤ 0.0005% Pb...........................≤ 0.0005% SO 4 ..........................≤ 0.005% Sr............................≤ 0.0005% Zn...........................≤ 0.0005% Polyethylene glycol 10,000 HR2-607 50% w/v solution 200 ml $80.00 Synonyms: PEG 10,000 H(OCH 2 CH 2 ) n OH M r 8,500 - 11,500 CAS [25322-68-3] EC No 500-038-2 Measured pH Range: 6.4 - 8.6 at 25°C Refractive Index Range: 1.40165 - 1.40254 at 20°C Conductivity Range: 39.4 - 48.3 mS at 25°C Polyethylene glycol 20,000 HR2-609 30% w/v solution 200 ml $80.00 Synonyms: PEG 20,000 H(OCH 2 CH 2 ) n OH Average M n ~16,000 - 24,000 CAS [25322-68-3] EC No 500-038-2 Measured pH Range: 4.6 - 10.2 at 25°C Refractive Index Range: 1.37438 - 1.37476 at 20°C Conductivity Range: 45.3 - 64.5 mS at 25°C Polyethylene glycol monomethyl ether 550 HR2-611 100% solution 200 ml $85.00 Synonyms: Methoxypolyethylene glycol or mono-Methyl polyethylene glycol or PEG MME 550 CH 3 O(CH 2 CH 2 O) n H M r 470 - 530 CAS [9004-74-4] EC No 215-801-2 Purity > 100.0% Density: 1.089 g/mL at 25°C (lit.) Measured pH Range: 8.5 - 10.5 at 25°C Refractive Index Range: 1.46144 - 1.46187 at 20°C Conductivity Range: 0.3 - 0.5 mS at 25°C Polyethylene glycol monomethyl ether 2,000 HR2-613 50% w/v solution 200 ml $85.00 Synonyms: Methoxypolyethylene glycol 2,000 or H 3 C OH mono-Methyl polyethylene glycol 2,000 or PEG MME 2,000 O CH 3 O(CH 2 CH 2 O) n H CAS [9004-74-4] EC No 215-801-2 n Purity > 95.0% Density: 1.094 g/mL at 25°C (lit.) Measured pH Range: 7.0 - 8.8 at 25°C Refractive Index Range: 1.40135 - 1.40161 at 20°C Conductivity Range: 28.0 - 60.2 mS at 25°C [ [ optimize crystallization grade reagents 41
Page 1 and 2: C R Y S T A L L I Z A T I O N volum
Page 3 and 4: table of contents PAGES 2 - 33 scre
Page 5 and 6: table of contents PAGES 5 pct pre-c
Page 7 and 8: pct Pre-Crystallization Test applic
Page 9 and 10: index f o r m u l a t i o n A1 A2 A
Page 11 and 12: crystal screen formulation A1 A2 A3
Page 13 and 14: PEGRx 1 formulation A1 A2 A3 A4 A5
Page 15 and 16: PEg/ion screen formulation A1 A2 A3
Page 17 and 18: Order Information Each Grid Screen
Page 19 and 20: saltrx 1 formulation A1 A2 A3 A4 A
Page 21 and 22: MEMBFAC formulation A1 A2 A3 A4 A5
Page 23 and 24: natrix formulation 1. 0.01 M Magnes
Page 25 and 26: crystal screen cryo formulation 1.
Page 27 and 28: Low Ionic Strength Screen applicati
Page 29 and 30: organic salts organic acids macromo
Page 31 and 32: Detergent Screen • detergent scre
Page 33 and 34: Heavy Atom Screen Pt (Platinum) 1.
Page 35 and 36: Crystal Image. Allan D’Arcy, Swit
Page 37 and 38: table of contents PAGES 36 - 37 cus
Page 39 and 40: StockOptions ADA - 185 ml $208.00 C
Page 41: table of contents Page Pre-Crystall
Page 45 and 46: Salts Ammonium acetate HR2-565 1.0
Page 47 and 48: Salts Lithium acetate dihydrate HR2
Page 49 and 50: Salts Potassium phosphate monobasic
Page 51 and 52: Salts Sodium tartrate dibasic dihyd
Page 53 and 54: Buffers Imidazole Buffer (Useful pH
Page 55 and 56: Optimize Silica - Reducing Hydrogel
Page 57 and 58: Silica Optimize Hydrogel - Oils Flu
Page 59 and 60: Optimize - Silica Hydrogel kit appl
Page 61 and 62: Nuclear receptor crystal. Gilbert B
Page 63 and 64: table of contents PAGES 62 stockopt
Page 65 and 66: StockOptions p H application n Cry
Page 67 and 68: stockoptions sodium citrate buffer
Page 69 and 70: stockoptions tris HO NH 2 OH pH 7.0
Page 71 and 72: "Butterfly"-crystal of hydroxynitri
Page 73 and 74: table of contents PAGES 72 - 77 24
Page 75 and 76: n Hanging Drop Crystallization n Si
Page 77 and 78: VDXm Plate application n Hanging d
Page 79 and 80: VDX Plate (Modular) applications n
Page 81 and 82: VDX48 Plate with sealant applicatio
Page 83 and 84: CrystalEX (Corning) application n S
Page 85 and 86: CrystalQuick 96 Well Sitting Drop P
Page 87 and 88: M R C 2 W e l l C r y s t a l l i z
Page 89 and 90: M R C U n d e r O i l 9 6 W e l l C
Page 91 and 92: Microbatch 96 Well Plate applicatio
Page 93 and 94:
Cover Slides & Related Tools Silico
Page 95 and 96:
Plain Glass Cover Slides applicatio
Page 97 and 98:
Duster - Canned Air application n D
Page 99 and 100:
9 well glass plate 9 W e l l G l a
Page 101 and 102:
Sealing Films, Tapes, Mats & Covers
Page 103 and 104:
AlumaSeal II sealing film and appli
Page 105 and 106:
Applicator for Dialysis Buttons app
Page 107 and 108:
Protein crystals. Kasumi Kobayashi,
Page 109 and 110:
table of contents PAGES 108 micro-t
Page 111 and 112:
Micro-Tools ii application n Precis
Page 113 and 114:
Small Forcep features n Length - 14
Page 115 and 116:
Locking Forcep features n Length -
Page 117 and 118:
Seeding tool application n Streak S
Page 119 and 120:
table of contents PAGES 118 - 120 c
Page 121 and 122:
CrystalCap Copper Magnetic ALS HT a
Page 123 and 124:
CrystalCap Magnetic systems Crystal
Page 125 and 126:
CrystalCap systems CrystalCap - C o
Page 127 and 128:
Adjustable Mounted CryoLoop applica
Page 129 and 130:
CryoLoops - 10 micron application n
Page 131 and 132:
CryoTong - L o n g application n Cr
Page 133 and 134:
Vial Clamp - Straight application n
Page 135 and 136:
Canes, Sleeves & Coders CryoCane ap
Page 137 and 138:
Foam Dewar application n Cryocrysta
Page 139 and 140:
table of contents PAGES 138 - 140 c
Page 141 and 142:
special glass 10 (SODA LIME GLASS)
Page 143 and 144:
wicks paper wicks applications n Re
Page 145 and 146:
Waxes & Clay beeswax applications n
Page 147 and 148:
Magnetic Mounts & Supports Adjustab
Page 149 and 150:
table of contents PAGES 148 xyz hea
Page 151 and 152:
XYZ Heated Goniometer Head supplies
Page 153 and 154:
XYZ Goniometer Head supplies Storag
Page 155 and 156:
Magnetic Base Support Magnetic Base
Page 157 and 158:
table of contents PAGES 156 xenon c
Page 159 and 160:
C a s e S t u d y : X e n o n D e r
Page 161 and 162:
table of contents PAGES 160 p ens 1
Page 163 and 164:
tough-tags® on a roll Tough-Tags®
Page 165 and 166:
Laser Cryo-Babies® and Cryo-Tags®
Page 167 and 168:
table of contents PAGES 166 macromo
Page 169 and 170:
protein Crystallization Strategies
Page 171 and 172:
Crystallography Made Crystal Clear
Page 173 and 174:
Macromolecular Crystallization Meth
Page 175 and 176:
Protein Crystallization n Editor: T
Page 177 and 178:
table of contents PAGES 176 glucose
Page 179 and 180:
lysozyme application n Crystallizat
Page 181 and 182:
Lipase B application n Protein stan
Page 183 and 184:
table of contents PAGES 182 - 183 p
Page 185 and 186:
Solutions for Crystal Growth Page 2
Page 187 and 188:
Oil Crystallization Drop Solutions
Page 189 and 190:
Solutions for Crystal Growth sittin
Page 191 and 192:
Page 193 and 194:
Solutions for Crystal Growth dialys
Page 195 and 196:
Solutions for Crystal Growth v i e
Page 197 and 198:
Solutions for Crystal Growth salt o
Page 199 and 200:
1 1.5 2 2.5 3 4 Solutions for Cryst
Page 201 and 202:
Solutions for Crystal Growth protei
Page 203 and 204:
Solutions for Crystal Growth What c
Page 205 and 206:
Solutions for Crystal Growth h o w
Page 207 and 208:
Solutions for Crystal Growth micros
Page 209 and 210:
Solutions for Crystal Growth p r o
Page 211 and 212:
Solutions for Crystal Growth temper
Page 213 and 214:
Crystal image. Anastasia Tziridis,
Page 215 and 216:
tips from ramc Tips from Recent Adv
Page 217 and 218:
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Page 221 and 222:
Page 223 and 224:
c r y s t a l l i z a t i o n tips
Page 225 and 226:
Page 227 and 228:
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Page 231 and 232:
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Page 237 and 238:
crystallization tips Solutions for
Page 239 and 240:
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Page 267 and 268:
pH BUFFER 1 2 3 4 5 6 7 8 9 10 11 1
Page 269:
Reagent Formula MW Temp (°C) g/100
Page 272 and 273:
index index A ADA..................
Page 274 and 275:
index index HR1-002................
Page 276 and 277:
index index HR4-CONTINUED HR4-116..
Page 278 and 279:
general information orders By phone
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