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13C phasing kit
application
n Produce heavy atom derivative of biological
macromolecules for phasing
features
n I3C kit for soaking or cocrystallization
n Phasing using SAD or SIRAS
n Ready-to-use, no weighing required
description
I3C can be used for heavy atom derivatization
of biological macromolecules for subsequent
single wavelength anomalous dispersion
(SAD) or single isomorphous replacement
plus anomalous scattering (SIRAS).
The two carboxylic acid groups and one
amino group of I3C can interact by way of
hydrogen bonds with both the backbone and
side chains of proteins. This can result in a
relatively highoccupancy of the bound ligand
I3C. The three iodine atoms per I3C molecule
provide for a strong anomalous signal.
The three iodine atoms in I3C form an equilateral triangle with 6.0 Å side lengths. These triangular structures
can readily be identified in the anomalous electron density map.
Kit Contents
• 12 aliquots of I3C (280 mg each) (5-Amino-2,4,6-triiodoisophthalic acid CAS number 35453-19-1)
• 12 aliquots of 2.0 M Lithium hydroxide (650 μl each)
• User Guide
References
1. A magic triangle for experimental phasing of macromolecules. Beck et al. Acta Cryst. D64, 1179, 2008.
2. Structure determination of the cancer-associated Mycoplasma hyorhinis protein Mhp37. Sippel et al. Acta Cryst. D64, 1172, 2008.
3. 5-Amino-2,4,6 triiodo-isophthalic acid monohydrate. Beck et al. Acta Cryst. E64:1286, 2008.
Order Information
Cat. No. Name Description Price
HR3-133 I3C Phasing Kit 24 tubes $155.00
screens
Crystal of cytochrome P450 Cyp121 from Mycobacterium tuberculosis with red color due
to the ferric heme iron. Crystal diffracts to 1.4Ang at Proxima1/Soleil, France.
Pascal Belin, iBiTec-s/CEA, Saclay, France.
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