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Here is a recipe to try: Mosaicity is about 0.5 t0 0.6 Reagent: Crystal Screen Cryo Reagent 23 Mix equal amounts of Glucose Isomerase and reagent. Vapor diffusion method. Mount crystal in CryoLoop. Mosaicity may be a bit more in this reagent and the unit cell will shrink a optimize c r y s t a l l i z a t i o n g r a d e reagents Protein crystal. Alexey Rak, Max-Planck-Institut fur Molekulare Physiologie, Department of Physical Biochemistry, Dortmund, Germany.
table of contents Page Pre-Crystallization Test XX PAGES 40 - 42 Index optimize - polymers XX 42 SaltRx optimize - organics (volatile) XX 42 PEG/Ion optimize Screen - organics (non-volatile) XX 43 - 49 Crystal optimize Screen - s a l t s 50 XX- 52 Crystal optimize Screen - buffers 2 52 Crystal optimize Screen - solubilizing Lite agents (ndsb) XX 53 Crystal optimize Screen - reducing Cryo agent XX 54 Grid optimize Screens - cryoprotectants 55 - 56 Quik optimize Screen - & oils Quik Optimize XX 57 Low optimize Ionic Strength - silica Screen hydrogel kit XX 57 Nucleic optimize Acid - lm Mini agarose Screen XX 58 Natrix optimize - izit crystal dye XX Membfac XX Stockoptions Screens XX Additive Screen XX Detergent Screen XX Heavy Atom Screens XX Cryopro-Cryoprotectant XX XX
Page 1 and 2: C R Y S T A L L I Z A T I O N volum
Page 3 and 4: table of contents PAGES 2 - 33 scre
Page 5 and 6: table of contents PAGES 5 pct pre-c
Page 7 and 8: pct Pre-Crystallization Test applic
Page 9 and 10: index f o r m u l a t i o n A1 A2 A
Page 11 and 12: crystal screen formulation A1 A2 A3
Page 13 and 14: PEGRx 1 formulation A1 A2 A3 A4 A5
Page 15 and 16: PEg/ion screen formulation A1 A2 A3
Page 17 and 18: Order Information Each Grid Screen
Page 19 and 20: saltrx 1 formulation A1 A2 A3 A4 A
Page 21 and 22: MEMBFAC formulation A1 A2 A3 A4 A5
Page 23 and 24: natrix formulation 1. 0.01 M Magnes
Page 25 and 26: crystal screen cryo formulation 1.
Page 27 and 28: Low Ionic Strength Screen applicati
Page 29 and 30: organic salts organic acids macromo
Page 31 and 32: Detergent Screen • detergent scre
Page 33 and 34: Heavy Atom Screen Pt (Platinum) 1.
Page 35 and 36: Crystal Image. Allan D’Arcy, Swit
Page 37 and 38: table of contents PAGES 36 - 37 cus
Page 39: StockOptions ADA - 185 ml $208.00 C
Page 43 and 44: Polymers Polyethylene glycol 300 HR
Page 45 and 46: Salts Ammonium acetate HR2-565 1.0
Page 47 and 48: Salts Lithium acetate dihydrate HR2
Page 49 and 50: Salts Potassium phosphate monobasic
Page 51 and 52: Salts Sodium tartrate dibasic dihyd
Page 53 and 54: Buffers Imidazole Buffer (Useful pH
Page 55 and 56: Optimize Silica - Reducing Hydrogel
Page 57 and 58: Silica Optimize Hydrogel - Oils Flu
Page 59 and 60: Optimize - Silica Hydrogel kit appl
Page 61 and 62: Nuclear receptor crystal. Gilbert B
Page 63 and 64: table of contents PAGES 62 stockopt
Page 65 and 66: StockOptions p H application n Cry
Page 67 and 68: stockoptions sodium citrate buffer
Page 69 and 70: stockoptions tris HO NH 2 OH pH 7.0
Page 71 and 72: "Butterfly"-crystal of hydroxynitri
Page 73 and 74: table of contents PAGES 72 - 77 24
Page 75 and 76: n Hanging Drop Crystallization n Si
Page 77 and 78: VDXm Plate application n Hanging d
Page 79 and 80: VDX Plate (Modular) applications n
Page 81 and 82: VDX48 Plate with sealant applicatio
Page 83 and 84: CrystalEX (Corning) application n S
Page 85 and 86: CrystalQuick 96 Well Sitting Drop P
Page 87 and 88: M R C 2 W e l l C r y s t a l l i z
Page 89 and 90: M R C U n d e r O i l 9 6 W e l l C
Page 91 and 92:
Microbatch 96 Well Plate applicatio
Page 93 and 94:
Cover Slides & Related Tools Silico
Page 95 and 96:
Plain Glass Cover Slides applicatio
Page 97 and 98:
Duster - Canned Air application n D
Page 99 and 100:
9 well glass plate 9 W e l l G l a
Page 101 and 102:
Sealing Films, Tapes, Mats & Covers
Page 103 and 104:
AlumaSeal II sealing film and appli
Page 105 and 106:
Applicator for Dialysis Buttons app
Page 107 and 108:
Protein crystals. Kasumi Kobayashi,
Page 109 and 110:
table of contents PAGES 108 micro-t
Page 111 and 112:
Micro-Tools ii application n Precis
Page 113 and 114:
Small Forcep features n Length - 14
Page 115 and 116:
Locking Forcep features n Length -
Page 117 and 118:
Seeding tool application n Streak S
Page 119 and 120:
table of contents PAGES 118 - 120 c
Page 121 and 122:
CrystalCap Copper Magnetic ALS HT a
Page 123 and 124:
CrystalCap Magnetic systems Crystal
Page 125 and 126:
CrystalCap systems CrystalCap - C o
Page 127 and 128:
Adjustable Mounted CryoLoop applica
Page 129 and 130:
CryoLoops - 10 micron application n
Page 131 and 132:
CryoTong - L o n g application n Cr
Page 133 and 134:
Vial Clamp - Straight application n
Page 135 and 136:
Canes, Sleeves & Coders CryoCane ap
Page 137 and 138:
Foam Dewar application n Cryocrysta
Page 139 and 140:
table of contents PAGES 138 - 140 c
Page 141 and 142:
special glass 10 (SODA LIME GLASS)
Page 143 and 144:
wicks paper wicks applications n Re
Page 145 and 146:
Waxes & Clay beeswax applications n
Page 147 and 148:
Magnetic Mounts & Supports Adjustab
Page 149 and 150:
table of contents PAGES 148 xyz hea
Page 151 and 152:
XYZ Heated Goniometer Head supplies
Page 153 and 154:
XYZ Goniometer Head supplies Storag
Page 155 and 156:
Magnetic Base Support Magnetic Base
Page 157 and 158:
table of contents PAGES 156 xenon c
Page 159 and 160:
C a s e S t u d y : X e n o n D e r
Page 161 and 162:
table of contents PAGES 160 p ens 1
Page 163 and 164:
tough-tags® on a roll Tough-Tags®
Page 165 and 166:
Laser Cryo-Babies® and Cryo-Tags®
Page 167 and 168:
table of contents PAGES 166 macromo
Page 169 and 170:
protein Crystallization Strategies
Page 171 and 172:
Crystallography Made Crystal Clear
Page 173 and 174:
Macromolecular Crystallization Meth
Page 175 and 176:
Protein Crystallization n Editor: T
Page 177 and 178:
table of contents PAGES 176 glucose
Page 179 and 180:
lysozyme application n Crystallizat
Page 181 and 182:
Lipase B application n Protein stan
Page 183 and 184:
table of contents PAGES 182 - 183 p
Page 185 and 186:
Solutions for Crystal Growth Page 2
Page 187 and 188:
Oil Crystallization Drop Solutions
Page 189 and 190:
Solutions for Crystal Growth sittin
Page 191 and 192:
Page 193 and 194:
Solutions for Crystal Growth dialys
Page 195 and 196:
Solutions for Crystal Growth v i e
Page 197 and 198:
Solutions for Crystal Growth salt o
Page 199 and 200:
1 1.5 2 2.5 3 4 Solutions for Cryst
Page 201 and 202:
Solutions for Crystal Growth protei
Page 203 and 204:
Solutions for Crystal Growth What c
Page 205 and 206:
Solutions for Crystal Growth h o w
Page 207 and 208:
Solutions for Crystal Growth micros
Page 209 and 210:
Solutions for Crystal Growth p r o
Page 211 and 212:
Solutions for Crystal Growth temper
Page 213 and 214:
Crystal image. Anastasia Tziridis,
Page 215 and 216:
tips from ramc Tips from Recent Adv
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c r y s t a l l i z a t i o n tips
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crystallization tips Solutions for
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pH BUFFER 1 2 3 4 5 6 7 8 9 10 11 1
Page 269:
Reagent Formula MW Temp (°C) g/100
Page 272 and 273:
index index A ADA..................
Page 274 and 275:
index index HR1-002................
Page 276 and 277:
index index HR4-CONTINUED HR4-116..
Page 278 and 279:
general information orders By phone
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