Culture Media for Microbiology CATALOGUE - Bartelt

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Scharlau, The wise choice Culture Media for Microbiology Chromogenic media CHROMagar Coliformes and E. coli CHROMAGAR CHROMagar E. coli CHROMAGAR E. coli E. coli bacteria are a fecal contamination indicator. The standard limits in food are approximately 50 E. coli bacteria per gram, therefore, it is important to detect and enumerate them correctly. Traditional methods for detecting E. coli are extremely tedious and usually require a heavy workload since many suspect colonies have to be tested. CHROMagar Liquid ECC CHROMAGAR CHROMagar Liquid ECC is used as a broth for the pad technique for the detection of E.coli and coliforms in water samples (10) . The inoculated filtration membrane is put on top of a pad presoaked with CHROMagar Liquid ECC. Art. No. 00000EL382 Microorganism Colony colour Sensitivity E. coli Blue 95% (9) Coliformes Mauve colour 94% (9) Proteus Colourless – Gram (+) Inhibited – ➜ (9) Alonso et al. 1999. Applied & Env. Microbiol. 65: 3746-3749. Presentation Dehydrated medium for 5 litres* Art. No. 00000EC168 00000EC169 Presentation Dehydrated medium for 5 litres* Dehydrated medium for 25 litres* AquaCHROM CHROMAGAR Microorganism Colony colour E. coli Blue Other gram (-) Colourless Gram (+) Inhibited ➜ (8) Alonso et al. 1996. J. Microbiol. Methods 25: 309-315. No bacteria E. coli Coliform (no E. coli) Bacteria (no coliform) Chromogenic medium for the detection of coliforms and E. coli. in water samples (100ml). By using 2 chromogenic substrates UV light is not needed to detect the presence of E. coli. Comes in 10 bags with 10 flasks of culture media to analyse 100ml of water. CHROMagar ECC CHROMAGAR E. coli Coliforms CHROMagar ECC will additionally show the other coliforms as red colonies. This is another useful indicator of questionable hygiene conditions. Art. No. 000000XO56 Newly included in this edition! CHROMagar STEC ✚ Presentation 100 tests CHROMAGAR Art. No. 00000EF322 00000EF323 Presentation Dehydrated medium for 5 litres* Dehydrated medium for 25 litres* Shiga-Toxin E. coli The kit CHROMagar STEC is a new chromogenic medium for the screening and detection of E. coli STEC (Shiga-Toxin producing E. coli), including E. coli O104, the microorganism related with recent outbreaks worldwide. In 24 hours this medium can detect the presence of the majority of E. coli STEC, not only E. coli O157. Art. No. Presentation 000ST160-1 Dehydrated medium for 1 litre** ** Kit CHROMagar STEC including the selective supplement CHROMagar STEC O104 (OG2011) ✚ Culture medium used in clinical applications. Culture medium used in industrial applications. * The presentation of culture media is in dehydrated form, every flask comes with the quantity of powder to reconstitute 5 or 25 litres of medium. NOTA 1: Colorex ® is the trademark for CHROMagar prepared media. NOTA 2: CHROMagar and Rambach are Dr. A. Rambach trademarks. Microorganism (with supplement OG2011) Colony colour STEC O104: H4 Mauve Other Colourless, blue or inhibited Microorganism (without supplement OG2011) Colony colour Most common Shiga-Toxin E. coli serotypes Mauve Other Enterobacteriaceae Colourless, blue or inhibited Gram (+) Inhibited www.scharlab.com scharlab@scharlab.com 43
Scharlau, The wise choice Culture Media for Microbiology Chromogenic media CHROMagar CHROMagar O157 ✚ CHROMAGAR CHROMagar Pseudomonas CHROMAGAR E. coli O157 The conventional medium for detection of E. coli O157, Sorbitol Mac Conkey Agar, has a poor specificity therefore creating a lot of false positives (Proteus, E. hermanii, etc.). Sorbitol Mac Conkey Agar is also difficult to read since the pathogen gives colourless colonies among red colonies. CHROMagar O157 is a chromogenic medium with easier detection of E. coli O157 as mallow colour colonies between blue and colourless colonies. Selectivity can be increased by adding potassium tellurite to our medium. Pseudomonas For the simultaneous detection and enumeration of Pseudomonas aeruginosa with markedly different colouring (blue colonies). One can use the membrane filtration method for detection from 100 ml of water, with the inoculated membrane placed on the agar plate. Art. No. 00000EE222 00000EE223 064-PA0080 Presentation Dehydrated medium for 5 litres* Dehydrated medium for 25 litres* 20 plates 90mm (Colorex ® ) Y Art. No. 00000PS822 Microorganism Some pseudomonas including P. aeruginosa Other Presentation Dehydrated medium for 5 litres* Colony colour Blue-green Generally colourless or inhibited Microorganism Colony colour Sensitivity & Specificity E. coli O157 Mauve colour 98% (2) E. coli spp. Blue – Other Blue, colourless or inhibited – ➜ (2) Bettelheim K.A. 1998. J. Clin. Microbiol. 85: 425-428. CHROMagar Vibrio CHROMAGAR V. vulnificus V. parahaemolyticus 00000VB912 V. parahaemolyticus, V. vulnificus & V. cholerae are pathogenic bacteria which can cause serious seafood poisoning. For the detection of those bacteria, traditional methods (TCBS) are time consuming, require heavy workload and are not very sensitive. On the contrary, CHROMagar Vibrio medium helps to easily differentiate V. parahaemolyticus, V. vulnificus & V. cholerae, from other Vibrio directly during the isolation step by colony colour with a sensitivity higher than conventional methods. Art. No. Microorganism Colony colour V. parahaemolyticus Mauve colour V. vulnificus and V. cholera Blue V. alginolyticus Colourless ➜ (6) Hara-Kudo et al. 2001. Applied & Env. Microbiol. 67: 5819-5823. Presentation 5 litres* CHROMagar Staph aureus ✚ CHROMAGAR S. aureus Staphylococcus aureus is a major pathogenic bacterium found in clinical field and in food industry. Nosocomial infections due to S. aureus create an increasing number of problems, which is why it is becoming more and more important to detect S. aureus. Mannitol fermentation based traditional media lead to many false positive and false negative results. CHROMagar Staph aureus has unrivalled sensitivity and specificity for detecting S. aureus after 24 hours. This makes unnecessary catalase and latex agglutination tests on non-S. aureus strains. Art. No. 00000TA672 00000TA653 Presentation Dehydrated medium for 5 litres* Dehydrated medium for 25 litres* Microorganism Colony colour Sensitivity Specificity Staphylococcus aureus Rose to mallow colour 95,5% (10) 99,4% (10) Other Blue, colourless, etc – – ➜ (10) Gailot et al. 2000. J. Clin. Microbiol. 38: 1587-1591. ✚ Culture medium used in clinical applications. Culture medium used in industrial applications. * The presentation of culture media is in dehydrated form, every flask comes with the quantity of powder to reconstitute 5 or 25 litres of medium. NOTA 1: Colorex ® is the trademark for CHROMagar prepared media. NOTA 2: CHROMagar and Rambach are Dr. A. Rambach trademarks. 44 www.scharlab.com scharlab@scharlab.com
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