Transfer Membranes

e i p e s
Recipes for Buffers used in Nucleic Acid Transfers *
Electrophoresis Buffers
1. TAE (1X) 40 mM Tris-acetate, 1 mM EDTA, pH 8.0 pH adjustments can be made with glacial acetic acid
2. TBE (1X) 45 mM Tris-base, 45 mM boric acid, 2 mM EDTA, pH 8.0
3. MOPS Buffer (10X) 50 mM sodium acetate, 10 mM EDTA, 0.2 M 3-morpholinopropanesulfonic acid, pH 7.0
4. E Buffer 5 mM sodium borate, 10 mM sodium sulfate, 1 mM EDTA, 50 mM boric acid, pH 8.2
Transfer and Washing Buffers
1. SSPE (1x) 0.18 M NaCl, 10 mM sodium phosphate, pH 7.7, 1 mM EDTA. To bring pH to 7.7, Na 2HPO 4 is
added to NaH 2PO 4
2. SSC (1x) 0.15 M NaCl, 15 mM sodium citrate, pH 7.0
3. TBS (1x) 50 mM Tris, 150 mM NaCl
Blocking Agents
1. Denhardt's Reagent 0.02 % Ficoll (mw = 400,000), 0.02 % polyvinylpyrolidone (mw = 40,000),
0.02 % BSA(Bovine Serum Albumin)
Sample Buffers
1. TE Buffer (1x) 10 mM Tris · HCl, pH 8.0, 1 mM EDTA
2. SET Buffer (1x) 0.1 M NaCl, 10 mM Tris · HCl, pH 8.0, 1 mM EDTA
pH adjustment to EDTA are made with NaOH
pH adjustments to Tris solution is made with HCl
* formulations recommended by AppliChem
Related Products: Chemicals Used For Blotting
AppliChem's General Catalog lists more than 2,700 biochemicals. Ask your local distributor for a copy of our catalog or
order it at www.applichem.de.
Acids and bases
Albumins
Amino acids
Antibiotics/Antimycotics
Biological Buffers
Cell culture powder media
Detergents
DNA and RNA isolation kits
Electrophoresis Chemicals
Enzyme inhibitors
Enzyme substrates
Enzymes
Indicators
Molecular biology grade reagents
Natural substance standards
PCR grade reagents
ready-to-use solutions and reagents
Salts
Solvents
Stains
Standard volumetric solutions
Vitamins
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44 Transfer Membranes • AppliChem © 2008