Transfer Membranes

Reprobe Nylon Positively Charged Transfer Membrane
● Ideal for use in multiple reprobings and nucleic acid transfers under alkaline conditions
● Specifically designed for multiple reprobings: Inherently charged; enhances nucleic acid binding – even under alkaline
conditions. Supported, has added strength and durability preventing distortion or contamination in multiple reprobings
● Greater binding capacity with a binding capacity of 450 mg/cm 2 , Reprobe Nylon Positively Charged Transfer Membrane
can bind a wide range of fragment sizes and can still be easily blocked for low background
● Excellent signal better retains DNA resulting in a strong signal using smaller quantities of DNA
● Hydrophilic, eliminates the need for wetting agents that can potentially interfere with biological processes
● Lot-to-lot consistency, quality checks ensure lot-to-lot consistency, both down and across the polyester web,
for dependable results every time
Applications
● Radiolabeled & non-radiolabeled detection systems
● Southern transfers
● Northern transfers
● Micro arrays
● Macro arrays
● Chemiluminescent systems
● Multiple Reprobings
● Alkaline Blotting
● UV Crosslinking
Product Description
Reprobe Nylon Positively Charged transfer membrane is an inherently charged, naturally hydrophilic nylon membrane, a
pure polymer impregnated by an inert polyester web. It is specifically designed to allow for numerous reprobings and use
in nucleic acid transfers under alkaline conditions. Reprobe Nylon Positively Charged transfer membrane has been tested to
provide consistent results through 12 or more reprobings.
The high binding capacity of 450 mg/cm 2 makes Reprobe Nylon Positively Charged transfer membrane ideal for all Southern
and Northern applications, including alkaline blotting. Reprobe Nylon Positively Charged transfer membrane is ideally suited
for all probes both radioactive and non-radioactive, including chemiluminescent and biotinylated detection systems.
Reprobe Nylon Positively Charged offers significantly increased binding, maximum “lot-to-lot” consistency, and excellent
signal retention. This higher sensitivity enables researchers to obtain reliable results using smaller quantities of DNA samples
and allows them to conserve valuable or expensive samples and save them for future use.
Given that smaller samples and higher density formats or micro arrays are becoming more common, the Reprobe Nylon
Positively Charged Transfer Membrane is especially well suited for genomic research. Even with small DNA samples, Reprobe
Nylon Positively Charged Transfer Membrane is able to retain a strong signal after multiple reprobings, providing easier
automated reading of signals and more discrimination.
The unique qualities of Reprobe Nylon Positively Charged Transfer Membrane provide a lower background and higher signal
assuring reliable results the first time a transfer is executed.
Product Characteristics
Sterilization
Gamma irradiation or Ethylene Oxide (EtO)
USP Class VI Testing
Passed
Thickness 65 – 125 µm
Extractables