ANIMAL BIOTECHNOLOGY Course Structure – at a Glance for M.Sc ...

ix. Somatic cell hybridization and production of hybridoma.
x. Screening of hybrids for production of monoclonal antibodies.
Suggested Readings
Kindt TJ, Goldsby RA & Osbrne BA. 2007. Kuby Immunology.
WH Freeman. Male D, Brostoff J, Roth DB & Roitt I. 2006. Immunology. Elsevier.
Spinger TA. 1985. Hybridoma Technology in Biosciences and Medicine.
Plenum Press.
BT 614 REPRODUCTIVE BIOTECHNOLOGY 2+1
Objective: Understanding in-vitro reproductive techniques for ovum and embryo
manipulation.
Theory
UNIT I
History, importance of assisted reproductive biotechnology in human and animal,
endocrine basis of reproduction, endocrine therapeutics. Reproductive ultrasonography
and its application in OPU and ETT.
UNIT II
Synchronization, Superovulation and embryo transfer technology, in vitro embryo
production and micromanipulation of embryos.
UNIT III
Different methods of gene transfer, sperm and embryo sexing, transgenesis, its
application and regulatory issue, production of transgenic livestock.
UNIT III
Cloning of domestic animals. Conservation of endangered species. Production and
characterization of embryonic stem cells. Cryopreservation of oocytes and embryos.
Practical
i. Synchronization and superovulation protocols.
ii. Collection of embryos using non-surgical procedures.
iii. Transferring embryos using non- surgical procedures.
iv. Oocyte/Embryo freezing protocols.
v. Oocyte collection and evaluation from slaughterhouse ovaries.
vi. In vitro fertilization protocols.
vii. Micromanipulation of early embryos.
viii. Monitoring reproductive organ using ultrasonography
Suggested Readings
Ball PJH & Peter AR. 2004. Reproduction in Cattle. Blackwell.
Gordon I. 2003. Laboratory Production of Cattle Embryos. CABI.
Gordon I. 2005. Reproductive Techniques in Farm Animals. CABI.
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