Experiments In Biotechnology - Student Welfare Division - BITS Pilani
Experiments In Biotechnology - Student Welfare Division - BITS Pilani
Experiments In Biotechnology - Student Welfare Division - BITS Pilani
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
<strong>BITS</strong> PILANI, DUBAI CAMPUS<br />
INSTRUCTION DIVISION<br />
SECOND SEMESTER 2012-2013<br />
Course Handout (Part II)<br />
Date: 03.02.2013<br />
<strong>In</strong> addition to part-I (General Handout for all courses appended to the timetable) this portion gives further<br />
specific details regarding the course.<br />
Course No : BIOT C346 (0 9 3)<br />
Course Title<br />
: <strong>Experiments</strong> in <strong>Biotechnology</strong><br />
<strong>In</strong>structor-in-charge : Dr. Trupti Gokhale<br />
<strong>In</strong>structors<br />
: Dr. Trupti Gokhale<br />
Scope and objective of the course:<br />
This course aims to familiarize with the recent techniques in the in the molecular biology.<br />
Course Pre/Co- requisite (if any)& Catalogue / Bulletin Description:Given in the Catalogue 2012 – 2013 CD<br />
Study Material:<br />
Text Books:<br />
1. EDD notes: Experimental Techniques: A laboratory manual (Practical notes for BIO G 642).<br />
2. Protocols will be provided in the laboratory<br />
Reference books:<br />
1. Molecular Cloning - by T. Maniatis, E.F. Fritsch and J. Sambrook, Cold Spring Harbower.<br />
2. Transcription and Translation a practical Approach of B.D. Hames and S.J. Higgins, 1984 IRL Press.<br />
3. Laboratory <strong>Experiments</strong> in Microbiology by Case and Johnson, 1984 Benamin & Cummings<br />
Publishing Ltd.<br />
4. Enzyme technology by Palmer<br />
5. Additional Protocols will be provided<br />
Course plan:<br />
Lec. No. Learning objectives References@<br />
(Text Book<br />
page numbers)<br />
1 Isolation of Eukaryotic genomic DNA with high yield and purity 6<br />
1 Quantitation of DNA and set up partial digestion for the construction of<br />
genomic DNA library.<br />
15,18,20<br />
1 Extraction, and detection of RNA 30<br />
1 Restriction digestion, Polymerase Chain Reactions 18, 25<br />
1 Cloning – Preparation of insert and vector, Ligation 35<br />
1 Transformation and screening of recombinants 10<br />
1 Expression and analysis of recombinant protein in E. coli by SDS – PAGE 37<br />
1 Reporter gene assays 13<br />
1 Immunological Experiment – Precipitation test. 41<br />
1 Immunological Experiment – Ouchterlony test 46<br />
1 Karyotyping Lab notes<br />
1 ELISA 43<br />
1 Southern blotting / Northern blotting (Demonstration) 22<br />
1 Western blotting (Demonstration) Lab notes<br />
Note: Out of this list, a maximum of 10 experiments will be conducted in the semester as per the<br />
availability of the consumables.<br />
Guide to Writing Lab Reports:<br />
The report must have to be written on hard bound, covered practical files. It should include the followings:<br />
a. Objective of the experiments<br />
b. Theory on which the experiment is based
c. Steps in the experimental procedure<br />
d. Results including all observation<br />
e. Conclusion<br />
f. Precautions to be taken<br />
Evaluation Scheme:<br />
EC No Components Duration Weightage% Date & Time Venue<br />
1 Viva-Voce 10 mins during every 20 Continuous<br />
experiment<br />
2 Lab Report Every experiment 20 Continuous<br />
3 Lab performance Continuous 20 Continuous<br />
4 Lab Comprehensive 3 hours 40 TBA<br />
To be<br />
announced<br />
later<br />
Mid-sem Grading:<br />
Mid-semgrading will be displayed after two evaluation components or earlier when- ever about 40 % of<br />
evaluation components are completed.<br />
Note:A student will be likely to get “NC”, if he / she<br />
• Doesn’t appear / appear for the sake of appearing for the evaluation components / scoring zero in<br />
pre-compre total.<br />
• Scoring zero in the lab component / Abstaining from lab classes throughout.<br />
Makeup and Attendance policies:<br />
Make-ups are not given as a routine. It is solely dependent upon the genuineness of the circumstances<br />
under which a student fails to appear in a scheduled evaluation component. <strong>In</strong> such circumstances, prior<br />
permission should be obtained from the <strong>In</strong>structor-in-Charge (I/C).The decision of the I/C in the above<br />
matter will be final.<br />
Attendance: Every student is expected to be responsible for regularity of his/her attendance in class<br />
rooms and laboratories, to appear in scheduled tests and examinations and fulfill all other tasks assigned<br />
to him/her in every course. A student should have a minimum of 50% of attendance in a course to be<br />
eligible to appear for the Comprehensive Examination in that course. For the students under the purview of<br />
Academic Counseling Board (ACB), the Board shall prescribe the minimum attendance requirement on a<br />
case-to-case basis. Attendance in the course will be a deciding factor in judging the seriousness of a<br />
student which may be directly / indirectly related to grading.<br />
General timings for consultation:<br />
Each instructor will specify his / her chamber consultation hours during<br />
which the student can contact him / her in his / her chamber for consultation. (For details see part II)<br />
General instructions:<br />
<strong>Student</strong>s should come prepared for classes and carry the text book(s) or material(s) as prescribed by the<br />
Course Faculty to the class.<br />
Notices:<br />
All notices concerning the course will be displayed on the respective Notice Boards.<br />
<strong>In</strong>structor-in-Charge<br />
BIOT C346<br />
Contact details<br />
Dr. Trupti Gokhale<br />
Assistant Professor<br />
Contact details: Chamber No:307 A1 Contact No: +971 4 4200700 Ext: 412 email: trupti@bits-dubai.ac.ae<br />
Mobile No: +971-50-1813394
Change language