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To study the inheritance in the F 2 population, F 2 seed of the crosses were
Ikram x inbred line 163A and Heinz H7155 x inbred line 163A were included with
77 and 95 plants of each F 2 population respectively. These materials were used from
the previous work of Fiorini (2008).
In addition to the previous materials, ‘New York’ and ‘Caline’ which have
only the Ph-1 resistance gene to late blight were used as standard varieties
susceptible control.
2.3 Mating design
Thirty seedlings of each of the 10 selected parents were grown in the
greenhouse during winter 2008 at Horta Velha, Vicosa, MG. Pollens from each
inbred line plants originated from the crossing (Solanum lycopersicum L.cv. Santa
Clara x Solanum habrochaites f. glabratum) were collected and bulked into plastic
plate, 4cm in diameter with the aid of vibration tool to help pollen-dispersal. Each of
the 3 commercial hybrids (Ikram, Heinz H7155 and Alambra) and three advanced
inbred lines (NC 1 CELBR, NC 2 CELBR and NC 25P) were crossed separately to
each of the five inbred lines following the II mating design (half dialle) where the P
and F 1 ’s were included only. Twenty pollinations for every cross was accomplished
during June/July, 2008. The fruit set of the cross NC 25P x inbred lines was low and
in addition a majority of fruits did not produce sufficient seeds (Table 1) so this
cross was eliminated from the field experiment evaluation. Table 1, shows the
names of parents and the amount of seed of each cross. Seed extraction was done
through fermentation for 48 h according to Giordano et al. (2003).
2.4 Field design
Seeds of F 1 were sown in 2 nd of March, 2009 in 200-cell trays using
commercial peat moss mixture as growing media fertilized once a week with 0.5%
solution of N:P:K (15:15:20). Thirty five day-old seedlings were transplanted in the
field. The applied experiment design was randomized complete block design
(RCBD) with 3 replicates, 5 plants per plot with distance 60 cm intra-row and 100
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