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~ G0UNmB¥ BER0BmS 15<br />

Current genetic conservation activities in situ and ex situ<br />

Since the early 1980s, gene reserves have been designated as follows: 6789.1 ha for Q. robur,<br />

265.2 ha for Q. petraea and 2820.4 ha for F. sylvatica. The distribution of gene reserves in<br />

Ukraine is given in Table 1. The largest reserve areas are concentrated in forest regions.<br />

Presently the inventory of gene reserves is being undertaken. This will provide the<br />

opportunity to estimate the current situation of genetic resources.<br />

For several years, studies on European beech diversity in the Carpathian region and<br />

adjacent territories have been carried out by the Ukrainian State University of Forestry and<br />

Wood Technology (Lviv), together with Technical University in Zvolen, Slovakia. Genetic<br />

diversity and differentiation of beech populations (20 European beech and 7 Crimean beech)<br />

have been studied by electrophoresis.<br />

Genetic differentiation was estimated on the basis of genetic distances. The results<br />

confirm a low degree of differentiation within F. sylvatica compared with F. orientalis, the<br />

values of subpopulation differentiation of European beech populations being only<br />

approximately 30% of the differentiation among F. oriental is populations. Within European<br />

beech populations from western Ukraine a slight differentiation between populations from<br />

the southwestern and northeastern macroslopes of the Carpathians, and plain populations<br />

from the northeastern limit of beech distribution range was observed. Crimean beech is also<br />

much more differentiated than Carpathian beech. Crimean beech occupies an eccentric<br />

position between both beech species; however, it is much more similar to F. orientalis than to<br />

F. sylvatica (Paule et al. 1993; Vysny et al. 1995; Gomory et al. 1998a, 1998b).<br />

The intensity of reproduction in beech root meristem cells was examined in cytological<br />

studies by the University together with the Institute of Forestry and Forest Melioration. The<br />

results led to the conclusion that some differences exist in the mitotic activity of root<br />

meristem cells of beech seedlings from different populations. Since the mitotic kinetics of<br />

the meristematic cells of all plants depend not only on ext~rnal factors but also on internal<br />

factors, it is quite possible that the results reflect the genetic diversity between populations<br />

(Kyrychenko et al. 1995).<br />

Clonal propagation of European beech and sessile oak is studied at the Laboratory of<br />

Tissue Culture. For this purpose explants were taken from different vegetative and<br />

generative plant parts of beech (buds of different-aged trees, hypocotyls, cotyledons)<br />

(Bazyuk and Fedyaj 1995).<br />

Relevant nature protection policies and activities<br />

Among the legislative acts related to conservation of genetic resources, the Law on Natural<br />

Reserves of Ukraine (of 5 May 1993) is in force. According to this law any activity which<br />

might affect negatively the state of protected areas is forbidden. The Committee of<br />

Environmental Protection is the administrative unit specialized in this field. The protection<br />

is implemented by the institutions on the territory in which they are situated. Protection of<br />

forest areas is carried out by regional forest offices.<br />

Table 1. Gene reserves of Social Broadleaves in Ukraine<br />

Quercus robur Quercus eetraea Faflus syJvatica<br />

Natural zone Area {ha} Number Area {ha} Number Area (ha) Number<br />

Mixed forest 2526.3 80 52.4 1 2.0 1<br />

Forest-steppe 3935.8 160 29.0 2 1789.8 60<br />

Steppe 269.0 1 128.0 7 0.0 0<br />

Carpathians 58.0 1 22.1 2 1028.6 7<br />

Crimea 0.0 0 33.7 4 0.0 0<br />

Total 6789.1 257 265.2 16 2820.4 68

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