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- G0llJNmBM BEB0SmS 39<br />

Current genetic conservation activities in situ and ex situ<br />

In situ<br />

Passive gene conservation of oaks and beech populations in situ takes the form of state<br />

reserves. The gene reserves (bases) are considered as an active way of gene conservation<br />

and reproduction. Gene reserves are groups of stands with a minimum surface area of<br />

100 ha of forest land. Regeneration is usually natural. If natural regeneration is not<br />

successful, it is possible to use artificial reproductive material but only with origins from<br />

these gene reserves. Suggestions for management methods in gene reserves are elaborated<br />

by the Institute.<br />

Ex situ<br />

The most important activities on ex situ gene conservation are grafting and establishment of<br />

clonal archives and seed orchards. This method is not used often for oak propagation,<br />

because of incompatibility problems. Only one seed orchard has been established and no<br />

others are planned. This method of conservation is more important for beech. We<br />

established clonal archives of beech from the seven forest vegetation belts only in the most<br />

polluted areas (Ore Mountains). Another method of ex situ conservation is the establishment<br />

of special plantations for obtaining secondary cuttings. Genebanks are used within the<br />

existing seed banks and tissue culture banks.<br />

Relevant nature protection policies and activities<br />

In 1992, a Law on Nature Protection was issued, and later in 1995 a new Forest Law (No.<br />

289). Both aim to conserve the remaining natural forest tree genepool.<br />

nee-improvement activities<br />

Tree-improvement activities are carried out on the basis of provenance tests. Practical<br />

implications of the provenance tests include rules for effective transfer of reproductive<br />

material, delimitation of seed zones, preparation and implementation of breeding<br />

programmes including different methods of vegetative reproduction (grafting, cuttings and<br />

in vitro methods).<br />

Use of reproductive material<br />

In the Czech Republic it is allowed to use reproductive material of spruce, pine and larch<br />

only from approved sources (approved seed stands, seed orchards, clonal archives). For all<br />

forest trees (including oaks and beech) a rule regarding the limitation of vertical transport of<br />

reproductive material is in force. This limit is plus or minus one forest vegetation belt (FVB).<br />

Beech origins from the seven FVBs are suggested as special climatic ecotypes.<br />

The proposed seed zones for oaks and beech (also for beech originating from the seven<br />

FVBs) have not been approved yet.<br />

Import of reproductive material of all forest tree species (including oaks and beech) from<br />

foreign countries is regulated by the Ministry of Agriculture. The Ministry also defined the<br />

areas where this reproductive material can be used.<br />

Institutions involved in genetic resources activities<br />

Most of the work is done by the Forestry and Game Management Research Institute. The<br />

central seed bank is managed by the state forest company and situated in the town TyniSten,<br />

Orlici. Both Forestry faculties (University Bmo and University Prague), the Academy of<br />

Science and the other state and private institutes and organizations are concerned with the<br />

problems of oaks and beech genetic resources to a limited extent. The Ministry of<br />

Environment and its organizations are interested in the conservation of oaks and beech in<br />

state nature reserves and national parks.

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