Handout in pdf

Increase of SMase activity in MCF7 cells
transfected with the six bSMase-GFP vectors
Specific activity (nmol/h/mg)
6
4
2
0
PM-control
PM-bSMase
* * *
12 24 48 72 96
Time (h)
50
40
30
20
10
0
Cyto-control
Cyto-bSMase
*
* *
12 24 48 72 96
Time (h)
30
20
10
0
Mito-control
Mito-bSMase
*
*
12 24 48 72 96
Time (h)
Specific activity (nmol/h/mg)
40
30
20
10
0
Golgi-control
Golgi-bSMase
*
*
* *
12 24 48 72 96
Time (h)
600
400
200
0
ER-control
ER-bSMase
* *
* * *
12 24 48 72 96
Time (h)
40
30
20
10
0
Nucleus-control
Nucleus-bSMase
*
* *
12 24 48 72 96
Time (h)