The Biuret Method for the Determination of Total Protein Using an ...
The Biuret Method for the Determination of Total Protein Using an ...
The Biuret Method for the Determination of Total Protein Using an ...
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St<strong>an</strong>dards 2 to 7 were measured at 540 nm using<br />
st<strong>an</strong>dard 1 as <strong>the</strong> reference sample, or bl<strong>an</strong>k. A linear fit<br />
was applied to <strong>the</strong> st<strong>an</strong>dard results in Table 2 to obtain<br />
<strong>the</strong> st<strong>an</strong>dard curve shown in Figure 4. <strong>The</strong> resulting<br />
calibration curve exhibits a linear relationship with a<br />
correlation coefficient (R 2 ) <strong>of</strong> 0.9996. <strong>The</strong> unknown<br />
sample was measured in Qu<strong>an</strong>tification mode. <strong>Using</strong><br />
<strong>the</strong> calibration curve <strong>the</strong> concentration <strong>of</strong> protein in<br />
<strong>the</strong> sample was calculated to be 1553 µg/mL, as shown<br />
in Table 2.<br />
Figure 4: <strong>Biuret</strong>-<strong>Protein</strong> Complex Spectrum <strong>an</strong>d Calibration Curve<br />
Solution Concentration (µg/mL) Absorb<strong>an</strong>ce<br />
St<strong>an</strong>dard 2 200 0.0238<br />
St<strong>an</strong>dard 3 400 0.0541<br />
St<strong>an</strong>dard 4 700 0.0862<br />
St<strong>an</strong>dard 5 1000 0.1304<br />
St<strong>an</strong>dard 6 2000 0.2514<br />
St<strong>an</strong>dard 7 3000 0.3817<br />
Unknown Sample 1553 0.1972<br />
Table 2: Results <strong>of</strong> <strong>Protein</strong> St<strong>an</strong>dards <strong>an</strong>d <strong>the</strong> Sample using <strong>Biuret</strong> <strong>Method</strong><br />
Conclusion<br />
Automated qu<strong>an</strong>titative <strong>an</strong>alysis <strong>of</strong> protein is per<strong>for</strong>med<br />
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<strong>an</strong>alysis into VISIONcollect s<strong>of</strong>tware saves time <strong>an</strong>d improves<br />
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<strong>of</strong> your methods.<br />
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