datasheet - Dolomite Microfluidics
datasheet - Dolomite Microfluidics
datasheet - Dolomite Microfluidics
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The <strong>Dolomite</strong> Centre Ltd<br />
Unit 1, Anglian Business Park, Orchard Road,<br />
Royston, Hertfordshire, SG8 5TW, UK<br />
T: +44 (0)1763 242491<br />
F: +44 (0)1763 246125<br />
E: sales@dolomite-microfluidics.com<br />
W: www.dolomite-microfluidics.com<br />
<strong>Dolomite</strong> <strong>Microfluidics</strong> – North America Office<br />
Blacktrace Inc, 29 Albion Place<br />
Charlestown, MA 02129, USA<br />
C: 617 803 6655<br />
T: 617 848 1121<br />
F: 617 500 0136<br />
E: salesus@dolomite-microfluidics.com<br />
Double Emulsion Chip<br />
Part No. 3200262<br />
product <strong>datasheet</strong><br />
page<br />
Description 2<br />
Benefits 3<br />
Specifications 3<br />
Sealing Interface 4<br />
Geometry 4<br />
Surface Coatings 5<br />
Droplet Formation 6<br />
Custom Options 6<br />
Optical Transmission 7<br />
The <strong>Dolomite</strong> Centre Limited Page 1 of 8
The <strong>Dolomite</strong> Centre Ltd<br />
Description<br />
Featuring both a hydrophobic and hydrophilic flow focussing droplet junction, this glass<br />
microfluidic chip enables the formation of water-in-oil-in-water droplets ranging from 80 to<br />
120μm (outer) and 50 to 90μm (inner) in diameter. The chip can also be reversed to<br />
create oil-in-water-in-oil droplets.<br />
Applications include drug delivery, high throughput screening, food based emulsions and<br />
bio-analytical studies.<br />
The Double Emulsion Chip is compatible with the Linear Connector 4-way (Part No.<br />
3000024) and the Chip Interface H (Part No. 3000155) for quick and reliable fluidic<br />
connection from tubing to chip.<br />
Top Left: Double Emulsion Chip (Part No. 3200262).<br />
Top Right: Microscope image of double emulsion droplets with an outer diameter of 100μm.<br />
Bottom Left: Chip Interface H with 2 x Linear Connectors 4-way.<br />
Bottom Right: Double emulsion of water-in-oil-in-water formed in the Double Emulsion Chip (Part No. 3200262).<br />
3200262_double_emulsion_chip_product_<strong>datasheet</strong>.doc Page 2 of 8
The <strong>Dolomite</strong> Centre Ltd<br />
Benefits<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
Features both a hydrophobic and hydrophilic flow focusing droplet junction<br />
Rapid generation of water-in-oil-in-water droplets in the size range of 80 to 120μm<br />
(outer droplet) and 50 to 90μm (inner droplet) in diameter<br />
Chip can be reversed to create oil-in-water-in-oil droplets<br />
Ideal for producing and analysing micro-emulsions<br />
Excellent chemical compatibility<br />
High visibility (excellent access for optics)<br />
Compatible with <strong>Dolomite</strong> Multiflux® Linear Connectors for quick and reliable<br />
fluidic connection<br />
Specifications<br />
Specification Double Emulsion Chip (Part No. 3200262)<br />
Channel cross-section at junction<br />
(depth x width)<br />
Wide channel cross-section (depth x<br />
width)<br />
Droplet size range<br />
Surface coating at 1 st junction<br />
Surface coating at 2 nd junction<br />
Surface coating transition<br />
100μm x 105μm<br />
100μm x 300μm<br />
Outer droplet: Ø 80 - 120μm<br />
Inner droplet: Ø 50 - 90μm<br />
Hydrophobic<br />
Hydrophilic<br />
Midpoint between 1 st junction and 2 nd junction (± 4 mm)<br />
Number of inputs 5<br />
Number of outputs 1<br />
Chip size (length x width x thickness)<br />
Chip top and base layer thickness<br />
Channel length after junction<br />
Back pressure with 100µl/min flow of<br />
water<br />
Surface roughness of channels (R a )<br />
Max. operating pressure<br />
Material<br />
Fabrication<br />
22.5mm x 15.0mm x 4mm<br />
2.0mm<br />
6.25mm<br />
0.02bar<br />
5nm<br />
up to 30bar<br />
Glass<br />
HF etching and thermal bonding<br />
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The <strong>Dolomite</strong> Centre Ltd<br />
Sealing Interface<br />
Connector body<br />
(PEEK)<br />
Seal<br />
Glass microfluidic chip<br />
Connector tubing PTFE, FEP<br />
Microchannel<br />
Geometry<br />
Channel at junction<br />
Wide channel<br />
100µm<br />
100µm<br />
105µm<br />
300µm<br />
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The <strong>Dolomite</strong> Centre Ltd<br />
Surface Coatings<br />
The glass channel surface is naturally hydrophilic. Hydrophobic coating has been<br />
selectively applied to junction 1 using a specialist fabrication process. This enables<br />
droplets of water-in-oil to be formed at junction 1. Droplets then pass through the 10 mm<br />
link channel to junction 2. At some point in the link channel, the surface properties change<br />
from hydrophobic to hydrophilic. This enables droplets of water-in-oil-in-water to be<br />
formed at junction 2.<br />
Oil-in-water-in-oil droplets can be generated using the same chip by reversing the chip in<br />
the holder.<br />
Water-in-Oil Droplets<br />
Water-in-Oil-in-Water Droplets<br />
Junction 1 - Hydrophobic<br />
Junction 2 - Hydrophilic<br />
The hydrophobic coating is resistant to organic solvents. It can be removed using acidic or<br />
basic solutions, for example a 0.1M Sodium Hydroxide for 24 hours.<br />
3200262_double_emulsion_chip_product_<strong>datasheet</strong>.doc Page 5 of 8
The <strong>Dolomite</strong> Centre Ltd<br />
Droplet Formation<br />
The size, consistency, and production rate of droplet formation is a function of several<br />
physical parameters, including:<br />
Channel size<br />
<br />
<br />
<br />
<br />
<br />
<br />
Viscosity and surface tension of the various fluids<br />
Presence of surfactants<br />
Miscibility of the fluids<br />
Use of hydrophobic or hydrophilic coating on the channel walls<br />
Total flow rate and relative flow rate of each fluid<br />
Flow stability<br />
To accelerate development work in droplet microfluidics, <strong>Dolomite</strong> offers a range of<br />
modular micro droplet systems featuring <strong>Dolomite</strong>’s industry leading microfluidic pumps,<br />
connectors and chips. The Droplet Advanced System is recommended for use with the<br />
small droplet chip for optimum results. This features the Mitos P-Pump, which provides<br />
stable, pulse-free flow for generation of droplets with extremely consistent diameters<br />
(monodisperse). Please contact <strong>Dolomite</strong> to configure a droplet system that fits your<br />
requirements.<br />
Mitos P-Pump, Flow Resistors and Droplet Chips featured in the Droplet Advanced System<br />
Custom Options<br />
Other chip configurations are available on request. If you would like to generate droplets<br />
of a different size, operate with challenging fluids or perform complex droplet functions,<br />
<strong>Dolomite</strong> can design the junction geometry required. The range of <strong>Dolomite</strong> services<br />
available covers all aspects of the development process from characterization of liquids<br />
for droplet generation to the design of commercial instruments in the field of droplet<br />
microfluidics. Please contact <strong>Dolomite</strong> to discuss your application.<br />
3200262_double_emulsion_chip_product_<strong>datasheet</strong>.doc Page 6 of 8
The <strong>Dolomite</strong> Centre Ltd<br />
Optical Transmission<br />
Spectral Transmittance of 2mm glass layer<br />
100<br />
Transmittance, %<br />
80<br />
60<br />
40<br />
20<br />
0<br />
250 350 450 550 650 750<br />
Wavelength, nm<br />
Optical Transmission of Glass<br />
3200262_double_emulsion_chip_product_<strong>datasheet</strong>.doc Page 7 of 8
The <strong>Dolomite</strong> Centre Ltd<br />
3200262_double_emulsion_chip_product_<strong>datasheet</strong>.doc Page 8 of 8
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