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Fix cells<br />
Stain with 1 °<br />
Antibody<br />
Infect 96-well MDCK cell monolayer<br />
with serial dilutions of virus sample<br />
Incubate 17-20 hrs<br />
Stain with 2 °<br />
Antibody<br />
Fluorescent Focus Assay<br />
Enumerate fluorescent foci