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Isocyte Technology<br />
� Laser is focused on a confined detection region at bottom of well<br />
� Depth of field allows large objects to be visualized in 3D<br />
� Fluorescence is collected by PMTs and digitized<br />
� Laser has very short dwell time - does not cause photobleaching<br />
PMT 1<br />
PMT 3<br />
Collection<br />
lens<br />
Dichroic mirror<br />
Laser<br />
Sample<br />
Scan lens<br />
Scanning mirror<br />
Emission filters<br />
PMT 2<br />
PMT 4
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