Model PS 3002 Electrophoresis Power Supply - Biometra

Model PS 3002
Electrophoresis Power Supply
CAT. NOS. 31067-234
23002-017

Table of Contents
1. Introduction ................................................................................................. 1
1.1 Notice to Customer................................................................................... 1
1.2 Warnings .................................................................................................. 1
2. Overview ....................................................................................................... 2
2.1 Description................................................................................................ 2
2.2 Set-Up ...................................................................................................... 2
3. Operating Instructions............................................................................ 4
3.1 Operation in Constant Voltage.................................................................... 4
3.2 Operation in Constant Current .................................................................... 5
3.3 Operation in Constant Watts....................................................................... 5
3.4 Choosing Safe Operating Limits ................................................................. 5
3.5 Automatic Crossover.................................................................................. 6
4. Troubleshooting Guide ........................................................................... 7
5. Related Products....................................................................................... 8
6. Additional Information ............................................................................ 9
5.1 Care and Handling.................................................................................... 9
5.2 Specifications............................................................................................. 9
5.3 Warranty.................................................................................................. 10
5.4 Declaration of Conformity and CE Mark ................................................. 10
Figure
1. Model PS 3002 Electrophoresis Power Supply ................................................. 3
GEL-MIX ® , HORIZON ® , RUNNING MATE, THE ILLUMINATOR, THE COURIER, TECH-LINE SM , and the Life
Technologies logo are marks of Life Technologies, Inc.
i

Introduction
1
1.1 Notice to Customer
!
This product is authorized for laboratory research use only. The product has not
been qualified or found safe and effective for any human or animal diagnostic or
therapeutic application. Uses for other than the labeled intended use may be a
violation of applicable law.
1.2 Warnings
1. DANGER! HIGH VOLTAGE! This power supply has been designed for use as a
source of DC power for electrophoresis. It is capable of generating lethal
currents. This unit should always be operated with extreme caution. Careless
handling could result in electrical shock.
2. Never operate damaged equipment. Do not use the unit without the cover in
place or with any possible short circuit. If the power supply emits smoke or
continually blows the main fuses, turn off the power supply and disconnect the
AC line and power cord.
3. Do not operate with connecting cables that have exposed live wires.
4. Always turn off the power supply before connecting or removing the power cords
or moving an electrophoresis apparatus.
5. Handle one power cord at a time, with one hand only, when connecting or
removing DC power cords at the power output terminals.
6. Do not immerse this unit in water.
7. Do not operate the unit in a damp, humid atmosphere or in a fashion where
condensed moisture may short out electrical components.
8. Do not connect the output to earth ground.
9. The AC power cord has a three-pronged plug which must be connected to a
grounded line voltage receptacle. Do not use a two-wire receptacle with an
adapter. This could create a serious electrical hazard for persons using the unit.
10. Use the correct voltage AC power outlet for the power supply. Be sure to always
plug a 110 VAC power supply into a 110-V line, and use a 240-V line with a 240
VAC unit.
1

2
Overview
2.1 Description
The Model PS3002 Electrophoresis Power Supply is intended to be used with
electrophoretic devices that operate below 3000 V DC and up to 300 W, and/or 300
mA. The unit includes two sets of output sockets which operate in parallel to provide
output in constant voltage, constant milliamps, or constant power (see figure 1).
This power supply has the ability to restart itself in the event of an AC mains power
failure.
2.2 Set-Up
Unpacking the Unit. Unpack and inspect the power supply unit carefully for any
damage. Do not use the unit if it is damaged. If damage is found, save the packing
material and report the problem to Life Technologies Customer Service.
Location. Make sure that the unit is set up in a location where it is protected from
physical damage, moisture, corrosive agents, and extreme temperatures. Make sure
that the fan at the rear is not obstructed; leave about 5-cm space behind the unit.
The unit should be readily accessible for safe operation.
Connection with the AC Mains. Connect the unit to the AC mains carrying the
appropriate specified voltage (V) in accordance with the rating label affixed to the
back of the unit. Ensure that the main receptacle and the power supply plug both
have the proper three-wire (grounded or earthed) connections.
2

Overview
2
Volts, Red
At Limit Light
Volts Display
Milliamps, Red
At Limit Light
Milliamps Display
Watts, Red
At Limit Light
Watts Display
DC Output Off,
Red Light
GIBCOBRL
4-mm Sockets
+
DANGER
HIGH VOLTAGE
-
LIFE TECHNOLOGIES
V3000 Volts
mA
300 mA W300 Watts
ELECTROPHORESIS POWER SUPPLY PS 3002
FAULT
STOP
START
PRESET
DC Output
Stop Key
DC Output
Start Key
DC Output On,
Green Light
Preset View Key
Volts Adjustment
Knob
Milliamps Adjustment
Knob
Watts Adjustment
Knob
Fault Light,
Yellow
Power On/Off
Switch
Figure 1. Model PS3002 Electrophoresis Power Supply
3

3
Operating Instructions
In order to achieve the greatest efficiency and effectiveness from this unit, read this
section in its entirety before setting up or operating the power supply unit and
carefully review figure 1 to ascertain the location of the power supply’s controls and
features.
3.1 Operation in Constant Voltage
1. Place the power supply on a sturdy, level surface in a safe, dry place away from
laboratory traffic. Controls must be easily accessible and sufficient space must
be available nearby for the electrophoresis unit(s) to be used with the power
supply. The fan intake in the back and exhaust on the bottom must remain
unobstructed.
Note: Allow at least 8 h for the unit to equilibrate if it is moved into or out of a
cold room. It must be completely dry before using.
2. The electrophoresis chamber should be set up, then filled with buffer, before
plugging the chamber’s leads into the appropriate positive and negative 4-mm
sockets on the power supply unit.
Note: Sample can be added before or after adjusting power supply settings.
3. Switch on the power supply using the Power On/Off switch. Once on, the red
DC Stop light will be illuminated. The Volts display, Milliamps display, and Watts
display will all indicate zero.
Notes on General Operation: The output of the unit is controlled by three
separate operating parameters. An operating limit, with a value above zero,
must be set for voltage, milliamps, and watts to generate an output. When the
power supply unit is activated, the output of the power supply will steadily
increase until an operating limit is reached. Once an operating limit is reached,
one of the three red At Limit lights will illuminate. The light will indicate which
operating limit has been reached first and which mode of control has been
established.
4. Using the Volts adjustment knob, select the operating voltage limit by turning the
knob until the desired value appears on the Volts display. Rotating the knob
slowly in a clockwise direction will increase the displayed value in 10-V
increments. Rotating the knob slowly in a counterclockwise direction will reduce
the displayed value in the same manner. As soon as the adjustment knob is
rotated, the output displays will show set values. When released, the set value
will remain illuminated for 3 seconds and then display the actual output values.
Note: The DC output must be off to change the output settings.
5. Using the Milliamps Adjustment knob and Watts Adjustment knob, select the
desired operating limits for milliamps and watts, respectively. The method used
to select these values is the same method used to select the operating voltage
limit.
Warning: The Model PS 3002 Power Supply Unit is capable of producing output
levels well in excess of the maximum safe operating limits for most
electrophoretic chambers. It is important to determine the safe operating limits
for milliamps and watts. Section 3.4 has been included to guide the user in
selecting proper operating parameters.
4

Operating
Instructions
6. Press the green DC Output Start key. The green DC Output On light should be
illuminated and the DC output, as indicated by the three displays, should rapidly
increase until the operating voltage limit is reached. At this point the red At Limit
light (volts) should be illuminated.
7. At the end of electrophoresis, turn off the power supply using the DC Output
Stop key and disconnect the leads.
3.2 Operation in Constant Current
The method used to set the power supply in constant current is the same as the
method used for constant voltage. The only difference involves the selection of an
operating limit for current which, as the output increases, is attained before the
operating limits for voltage or watts.
3.3 Operation in Constant Watts
The method used to set the power supply in constant watts is the same as the
method used to set constant voltage with one notable difference. The difference
involves the selection of an operating limit for watts which, as the output increases,
is attained before the operating limits for voltage or current.
3.4 Choosing Safe Operating Limits
Electrophoretic chambers are generally designed for a relatively specific purpose.
For example, submarine chambers use agarose gels to separate DNA or RNA
fragments, while a DNA sequencing chamber is almost always used to separate
DNA in a denaturing polyacrylamide gel. In each case, the voltage, milliamp, and
wattage requirements are well defined within a reasonable range of values. In cases
such as these, the user can safely assume that the manufacturer has designed the
chamber to withstand the voltage and heat energy necessary to perform the
electrophoretic separation when standard protocols are followed.
Some types of electrophoretic chambers are specifically designed to be
multipurpose devices. For example, a vertical slab gel chamber could be used for
anything from DNA sequencing to isoelectric focusing, depending on the gel type
and buffer system used. Choosing safe operating limits for a chamber of this type
requires a higher degree of caution. Chamber manufacturers normally rate their
product for maximum voltage and/or maximum wattage. Whenever possible,
contact the manufacturer and request this information.
Whether you use special purpose or multipurpose chambers, the maximum
operating temperature the chambers will withstand is a critical aspect of safe
operation. Most electrophoretic chambers (with a few notable exceptions) are made
of acrylic and plastic and should operate below a temperature of 35°C. If there is a
lack of information about the capabilities of the chamber being used, regular
monitoring of the operating temperature is recommended. This should be
accomplished without coming into physical contact with the chamber when voltage
is applied. Use the procedure listed below to select operating parameters.
1. Set voltage as described in steps 1–4 for operation in Section 3.1.
2. Using the Milliamps and Watts adjustment knobs, select the maximum operating
limits of 300 mA and 300 W, respectively.
3. Press the green DC Output Start key. The green DC Output On light should
illuminate and the DC output, as indicated by the three displays, should rapidly
increase until the operating voltage limit is reached. At this point the red At Limit
light (volts) should be the only ‘at limit’ light illuminated.
4. Once the power supply has reached the operating voltage limit, note the actual
number of milliamps and watts displayed and press the red DC Output Stop key.
Confirm that the red DC Output Off light is illuminated.
3
5

Operating
Instructions
5. Add 15 mA and 10 W to the actual values noted earlier. Using the adjustment
knobs, set the operating limits to the new values.
6. Press the green DC Output Start key. The green DC On light should illuminate
and the DC output displays should rapidly increase until the operating voltage
limit is reached. The red At Limit light for volts should be the only one
illuminated.
Note: It may be necessary to readjust the watt or milliamp operating limit during
electrophoresis to ensure that the entire procedure is performed at a constant
voltage. This readjustment, if necessary, is required to compensate for large
changes in resistance which occur during certain types of electrophoresis (see
Section 3.5).
Note: To view the output settings during electrophoresis, press the yellow
preset key. The LED’s will display the set output values as long as the key is
depressed. Once released, the LED’s will display the set values for 3 seconds
and then switch to the actual output values.
3.5 Automatic Crossover
For some electrophoresis conditions, a function of this power supply unit known as
automatic crossover will occur. Automatic crossover is the ability of the unit to
change the mode of operation during the course of an experiment. As conditions of
buffers and gels change during a run, a constant voltage run may, for example,
become limited by current or power if the limit for either of these other values is
reached. This automatic crossover process is used to protect both the experiment
and equipment.
6

Troubleshooting Guide
4
This power supply is equipped with both audible and visual alarms to help you
troubleshoot. Many operating problems may be solved by reading and carefully
following the instructions in this manual. Some suggestions for troubleshooting are
given below. Should these suggestions not resolve the problem, contact the TECH-
LINE SM
at the number listed on the back of this manual. Should the power supply
need to be returned for service, contact Customer Relations for instructions. Include
a full description of the problem.
Symptom Problem Comment
An alarm sounds and Open connection Depress the red DC Output Stop
is accompanied by the
key to cancel the alarm mode.
illumination of the yellow
fault light.
This power supply is equipped
with a load sensing interlock
which is designed to inhibit the
generation of high voltage
whenever an open connection
is detected at the output.
An open connection could be
caused by any of the following
conditions:
1. A broken electrode within the
electrophoretic chamber.
2. A broken wire within a
connecting cord.
3. Insufficient buffer levels in
the electrophoretic chamber.
4. Loose connectors.
5. Electrochemical energy
stored within the chamber.
Ground leakage is
detected
Turn off the unit.
The voltage and wattage Ground leakage is Do not operate the power supply
displays hold their last detected when this condition exists.
value and the milliamps
For further assistance, call the
display is blank.
TECH-LINE.
Alarm sounds for Power on restart Auto-restart will occur at the end
10 seconds. of a mains power failure. The
alarm will clear itself.
7

5
Related Products
Product
Cat. No.
Model 4001 Electrophoresis Power Supply 31067-069
Model 4001P Programmable Electrophoresis Power Supply 31067-119
Model S2 Sequencing Gel Electrophoresis System 21105-010
Model SA-32 Sequencing Gel Electrophoresis System 31096-019
Model SA-60 Sequencing Gel Electrophoresis System 31096-035
Model SA-88 Sequencing Gel Electrophoresis System 31096-050
GEL-MIX ® 6 6% Sequencing Gel System 15543-010
GEL-MIX 8 8% Sequencing Gel System 15545-015
GEL-MIX RUNNING MATE 10X TBE Buffer 15546-013
dsDNA Cycle Sequencing System (100 reactions) 18196-014
Model GDS2000 Gel Dryer 10384-030
Model GD40/50 Gel Dryer 10384-014
THE ILLUMINATOR Autoradiographic Filmviewer 10241-313
Intensifying Screen 35!43 10241-131
THE COURIER 35!43 Film Cassette 10241-057
S2 Casting Clamp 21105-432
S2 Casting Clamp (2/pk.) 21105-473
Large Vertical Multicaster 21220-207
Glass Plate Wash Stand 21105-655
Accessories or Replacement Parts:
Fuse, T 4.0 A, 250 V 31067-275
Fuse, T 2.0 A, 250 V 31067-283
8

Additional Information
6
5.1 Care and Handling
This power supply uses all solid-state components and should require no
maintenance or recalibration under normal use. If the unit must be returned for
repair, contact Life Technologies’ Customer Service Department for shipping
instructions. Please include a full description of the problem. As with any laboratory
instrument, adequate care ensures consistent and reliable performance.
The power supply must not be immersed in water. The unit can be wiped with a soft
cloth, dampened with water and a nonabrasive, mild soap or detergent. Do not
allow water to enter the case. Abrasive cleaners, window sprays or rough cloths
may damage the surface and should be avoided. Grease and oils may be removed
using a light application of hexane, kerosene or aliphatic naphtha. Do not expose
the surface to phenol, acetone, benzene, halogenated hydrocarbon solvents or
undiluted laboratory alcohols. Avoid prolonged exposure of the power supply to UV
light. A soft, dry cloth may be used to dry the unit.
5.2 Specifications
Net Weight: ........................................................................................................... 6 kg (13.2 lb)
Dimensions: ............................................................................................ 27.2 " 34.7 " 11.0 cm
(D " W " H).............................................................................................. (10.7 " 13.6 " 4.3 in.)
Installation Category ........................................................................................................Type 2
Construction: ............................................ Polycarbonate plastic and epoxy-painted aluminum
Power Requirement: .................................................................. 110/220 VAC 50/60 Hz 750 W
Mains Fuse Rating:
110 ±10 vrms operation .......................................................................................... T4A/250V
220 ±20 vrms operation ...........................................................................................T2A/250V
Maximum Voltage:.......................................................................................................... 3000 V
Maximum Power:............................................................................................................. 300 W
Maximum Current: ......................................................................................................... 300 mA
Regulation:............................................................................................................................ 1%
Accuracy:............................................................................... ± 1.5% full scale for each display
Number of Output Terminals: ........................................... Two recessed sets of 4-mm sockets
Safety Interlock: ................................................... Load sensing shut-down-on-disconnect. DC
Output Start key activation necessary to begin
voltage generation. In the event of shutdown due to
power interruption, automatic restart is provided.
Ground Leakage: ................ Leakage of > 500 µA will interrupt the generation of high voltage.
9

Additional
Information
5.3 Warranty
Life Technologies, Inc. warrants apparatus of its manufacture against defects in
materials and workmanship, under normal service, for one year from the date of
receipt by the purchaser. This warranty excludes damages resulting from shipping,
misuse, carelessness, or neglect. Life Technologies’ liability under the warranty is
limited to the repair of such defects or the replacement of the product, at its option,
and is subject to receipt of reasonable proof by the customer that the defect is
embraced within the terms of the warranty. All claims made under this warranty
must be presented to Life Technologies within one year following the date of
delivery of the product to the customer.
This warranty is in lieu of any other warranties or guarantees, expressed or
implied, arising by law or otherwise. Life Technologies makes no other
warranty, expressed or implied, including warranties of merchantability or
fitness for a particular purpose. Under no circumstances shall Life
Technologies be liable for damages either consequential, compensatory,
incidental or special, sounding in negligence, strict liability, breach of
warranty or any other theory, arising out of the use of the product listed
herein.
Life Technologies reserves the right to make improvements in design, construction,
and appearance without notice.
5.4 Declaration of Conformity and CE Mark
Note: The information outlined in this section applies only to customers located in
the European Union (EU). The EU is currently comprised of 15 member countries.
This laboratory apparatus is identified with the CE mark. This mark indicates that the
product complies to the following EU Directives and Standards:
Application of Council Directive(s):
73/23/EEC Low Voltage Directive
89/336/EEC Electromagnetic Compatibility
Standards:
EN 61010-1:1993 Product Safety
EN 50081-1:1992 Emissions
EN 50082-1:1992 Immunity
EU Representative:
Life Technologies Ltd.
EU Address:
3 Fountain Dr.
Inchinnan Business Park
Paisley, PA49RF Scotland
A copy of the Declaration of Conformity certificate is available upon request.
10

Part No. 50853
Lot No. JE9P05-0999