GENERAL BIOLOGY LAB 1 (BSC1010L)

Important Notes:
• Each group will test the optimal temperature for one amylase type and then
will repeat the experiment using the amylase from the other source. Your
instructor will assign the enzyme that your group should start with.
• At the end of the experiment, your group should have 2 data sets, one for
each type of amylase (fungal and human).
• To determine the optimal temperatures for both enzymes, you will need to
generate a class data set by combining your group’s results with the data
collected by the other groups in your class. These data sets
Procedure:
I. Experimental Setup:
1. Place a napkin/paper under the spot plates (Figure 5) and across the top
write Temperature (0°, 40°, 60°, 95° Celsius) and on the side write Time (0, 2,
4, 6, 8, 10 min).
Figure 5. Spot plate setup
2. Obtain 4 test tubes and label each with a different temperature
(0°, 40°, 60°, 95° Celsius), enzyme source (H – human and F –
fungal) and your group number.
0° - H – Grp1
3. Obtain another 4 test tubes and label these with a different
temperature, enzyme source (H or F), your group number and
the letter S (for starch solution).
4. Add 5mL of 1.5% starch solution into each of the test tubes
labeled S.
II. Effect of Temperature on Amylase Activity
5. Add 1mL of amylase into each of the test tubes that do not
contain starch.
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