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Sept 2001 Rough Draft OFFM Policy Letter 02-1 - Office of Research ...

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techniques that will result in increased algal biomass productivity in outdoor cultivation<br />

environments relevant to commercial scales (i.e., one (1) acre cultivation equivalent).<br />

Achieving a target for a minimum ash free dry weight (AFDW) productivity will not be the only<br />

variable that will enable meeting the goal <strong>of</strong> 2,500 gallons <strong>of</strong> bi<strong>of</strong>uel intermediate per acre per year<br />

by 2018. However, achieving high levels <strong>of</strong> algal biomass productivity continues to be one <strong>of</strong> the<br />

most significant contributors to achieving an economically viable algal bi<strong>of</strong>uels industry. BTO’s<br />

baseline analysis results show that conservative modeled productivities (13 grams per meter<br />

squared per day – g/m2/day on an annual average basis) are associated with high resource use, high<br />

costs, and high GHG emissions for algal bi<strong>of</strong>uel systems. Priority Area 1 is focused on<br />

demonstrating increased algal biomass productivity that, in combination with improvements in<br />

other downstream processes, will meet the bi<strong>of</strong>uel intermediate productivity goal <strong>of</strong> 2,500 gallons<br />

per acre per year by 2018.<br />

It is recognized that productivities above 25 g/m2/day are achievable in the lab and that advanced<br />

biotechnology may further increase this, but a clear barrier exists in translating laboratory success<br />

to demonstrated, scalable, outdoor cultivation environments that capture all <strong>of</strong> the variables not<br />

present in laboratory systems. Thus, it is expected that the research conducted in the selected<br />

projects will include an iterative process whereby the results obtained from experiments in outdoor<br />

environments will be used to inform the laboratory experiments and vice versa. This continuous<br />

feedback loop is expected to expedite the lessons learned and ensure they are relevant for large<br />

scale algal bi<strong>of</strong>uel production.<br />

Important aspects <strong>of</strong> Priority Area 1 include:<br />

Production <strong>of</strong> algal feedstock refers to the cultivation <strong>of</strong> both micro- and macro-alga, as well<br />

as cyanobacteria. (See Appendix A for a definition <strong>of</strong> allowable algal types.)<br />

Improvements to increase algal biomass productivity may include, but are not limited to:<br />

advances made to systems biology approaches; gains in knowledge related to fundamental<br />

algal processes (e.g., photosynthesis); strain improvement from breeding to modification<br />

and/or genetic engineering; improvements in cultivation strategies, such as crop protection,<br />

water and nutrient management, carbon dioxide delivery and utilization, light optimization,<br />

temperature management, and seasonal succession; and cultivation infrastructure<br />

engineering to maximize biomass yields while minimizing land, capital, and operating costs.<br />

Acceptable algae cultivation systems include open ponds, attached growth systems, and<br />

closed photobioreactors (PBRs), combinations <strong>of</strong> these systems, or other systems with<br />

similarly justifiable scalable potential.<br />

There are a wide variety <strong>of</strong> processes that may use various components <strong>of</strong> the algal<br />

feedstocks on the path towards commercialization <strong>of</strong> bi<strong>of</strong>uels, and possibly bioproducts. As<br />

such, all <strong>of</strong> the major bulk biochemical components (lipid, protein, carbohydrate, and ash)<br />

are targets for improvements if they can be clearly demonstrated to lead to increased yields<br />

<strong>of</strong> bi<strong>of</strong>uel intermediates. Thus, the productivity <strong>of</strong> all <strong>of</strong> these components will be<br />

considered under this FOA.<br />

It is expected that applicants will have already identified: promising strain(s) or partnered<br />

appropriately to gain access to identified strains; strain characteristic(s); and cultivation<br />

technique(s) backed by supporting data that demonstrate(s) a significant potential to<br />

increase biomass productivity. This information must be included in the application.<br />

Standardized analytical methods for measuring the key biochemical constituents are vital to<br />

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