Fluorophores - Integrated DNA Technologies

A n t i s e n s e O l i g o n u c l e o t i d e s
Phosphorothioates and Chimeric Oligos
LNA, 2'-O-Methyl RNA, and 5-Methyl dC
References:
1. Role of RNase H in hybridarrested
translation by antisense
oligonucleotides. Walder, R.Y. and
Walder, J.A. Proc. Natl. Acad. Sci.
USA, 85:5011 (1988).
2. Targeted degradation of
mRNA in Xenopus oocytes and
embryos directed by modified
oligonucleotides: studies of An2 and
cyclin in embryogenesis.
Dagle, J.M., Walder, J.A.and Weeks,
D.L. Nucleic Acids Res., 18:4751
(1990).
3. Antisense inhibition of
gene expression in cells by
oligonucleotides incorporating
locked nucleic acids: effect fo
mRNA target sequence and chimera
design. Braasch, D.A., Liu, Y.,
and Corey, D.R. Nucleic Acids
Research, 30:5160-5167 (2002).
4. Design of antisense oligos
stabilized by locked nucleic acids.
Kurreck, J., Wyszko, E., Clemens,
G., and Erdmann, V.A. Nucleic Acids
Research, 30:1911-1918 (2002).
5. Comparison of different antisense
strategies in mammalian cells using
locked nucleic acids, 2'-O-methyl
RNA, phosphorothioates and small
interfering RNA. Grunweller, A.,
Wyszko, E., Bieber, B., Jahnel, R.,
Erdmann, V.A., and Kurreck, J.
Nucleic Acids Research, 31:3185-
3193(2003).
4 Functional Genomincs
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