Fluorophores - Integrated DNA Technologies

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Molecular Beacons Please see our website at www.idtdna.com for more possible reporter / quencher combinations. 5 <strong>DNA</strong> & RNA Probes for Fluorescence-Based Applications Technical Note! The signal-to-noise ratio (STNR) for each Molecular Beacon is determined by hybridizing it to its complementary sequence and comparing fluorescence to to background. For FAM -labeled Molecular Beacons, guaranteed STNR is >15. For all other dyes, a STNR of >10 is required to pass quality control. References: 1. Thermodynamic basis of the enhanced specificity of structured <strong>DNA</strong> probes. Bonnet et al. Proc Natl Acad Sci USA 96: 6171–6176 (1999). Reporter: 5' 6-FAM Quencher Scale Guarantee Price 3' Dabcyl 250 nmole 10 nmoles 1 µmole 40 nmoles 3' Black Hole 250 nmole 10 nmoles Quencher ® -1 1 µmole 40 nmoles Reporter: 5' TET Quencher Scale Guarantee Price 3' Dabcyl 250 nmole 10 nmoles 1 µmole 40 nmoles 3' Black Hole 250 nmole 10 nmoles Quencher 1 1 µmole 40 nmoles Reporter: 5' HEX Quencher Scale Guarantee Price 3' Dabcyl 250 nmole 10 nmoles 1 µmole 40 nmoles 3' Black Hole 250 nmole 10 nmoles Quencher ® -2 1 µmole 40 nmoles Reporter: 5' Cy3 Quencher Scale Guarantee Price 3' Black Hole 250 nmole 5 nmoles Quencher ® -2 1 µmole 20 nmoles Reporter: 5' Cy5 Quencher Scale Guarantee Price Phone: 03-5248-3066 Fax: 03-5248-2930 e-mail: oligo@mbl.co.jp 38 3' Black Hole 250 nmole 5 nmoles Quencher ® - 2 1 µmole 20 nmoles
Modifications Technical Note! 5' 5' Amino Modifier C6 (phosphoramidite) Int Internal amino-C6-dT (phosphoramidite) 3' 3' Amino Modifier (CPG) 6 Modifications 39
Page 2 and 3: 1 General Information
Page 4 and 5: Table of Contents 1 General Informa
Page 6 and 7: The IDT Advantage 1 General Informa
Page 8 and 9: Custom DNA Synthesis Synthesis Scal
Page 10: Custom DNA Synthesis in Plates
Page 13 and 14: Custom RNA Synthesis and Purificati
Page 15 and 16: Functional Genomics Antisens
Page 17 and 18: A n t i s e n s e O l i g o n u c l
Page 19 and 20: 4 Functional Genomincs
Page 21 and 22: RNA Interference 4 Functional Genom
Page 23 and 24: Purity and QC Duplex Yie
Page 25 and 26: 4 Functional Genomincs
Page 27 and 28: DNA-Directed RNA Interference (ddRN
Page 29 and 30: 4 Functional Genomincs 27
Page 31 and 32: Fluorescent DNA and RNA Probes 5 DN
Page 33 and 34: Dual-Labeled DNA Probes Reporte
Page 35 and 36: Reporter: 5' CAL Fluor ® Red 610 Q
Page 37 and 38: Quencher: 5' Iowa Black RQ Reporte
Page 39: Dual-Labeled RNA Probes - Only at I
Page 43 and 44: Attachment Chemistry / Linkers Amin
Page 45 and 46: Attachment Chemistry / Linkers Biot
Page 47 and 48: Attachment Chemistry / Linkers Thio
Page 49 and 50: Fluorophores 5' MW: 883.9 Int. MW:
Page 51 and 52: Fluorophores Bodipy® Dyes Bodipy
Page 53 and 54: Fluorophores Cy Dyes Cy3 * 5'
Page 55 and 56: Fluorophores Fluorescein dT * MW: 8
Page 57 and 58: Fluorophores 5' MW: 673.5 Int. MW:
Page 59 and 60: Fluorophores Rhodamine Red -X NHS
Page 61 and 62: Fluorophores TET Ab max 522 nm Em
Page 63 and 64: Dark Quenchers Black Hole Quencher
Page 65 and 66: Spacers Spacer 18 MW: 344.3 SCALE
Page 67 and 68: Modified Bases Inverted dT MW: 304.
Page 69 and 70: Modified Bases 5-Nitroindole MW: 34
Page 71 and 72: Modified Bases Iso-dG* 5' MW: 329.2
Page 73 and 74: RNaseAlert Substrate and Nuclease
Page 75 and 76: Nuclease-free Buffers & Reagents 7
Page 77 and 78: ® Nucleic Acid Labeling System ®
Page 79 and 80: miRNA Cloning Linker
Page 81 and 82: Microarray-Related Products DNA
Page 83 and 84: Custom Services 8 Custom Services 8
Page 85 and 86: Packaging and Formulation
Page 87 and 88: Service Pricing Lab-Ready O
Page 89 and 90: Usage, Warranty and Licenses Usage
Page 91 and 92:
10 Index Index General Information
Page 93 and 94:
10 Index Index ReadyMade Primers, c
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Fluorophores - Integrated DNA Technologies

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