Fluorophores - Integrated DNA Technologies
Fluorophores - Integrated DNA Technologies
Fluorophores - Integrated DNA Technologies
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Attachment Chemistry / Linkers<br />
Thiol Modifier C6 S-S<br />
MW: 328.4<br />
SCALE 5' INT. 3'<br />
100 nmole - -<br />
HO<br />
S<br />
S<br />
250 nmole - -<br />
1.0 µmole - -<br />
O<br />
O P O -<br />
<strong>Fluorophores</strong><br />
Alexa Fluor® Dyes<br />
O<br />
3'<br />
6 Modifications<br />
<br />
<br />
<br />
Alexa Fluor® 488 NHS Ester *<br />
5' MW: 694.6<br />
Int. MW: 973.9<br />
Ab max 492 nm Em max 517 nm 3' MW: 694.6<br />
SCALE 5' INT. 3'<br />
100 nmole <br />
SO 3<br />
- SO 3<br />
-<br />
H 2 N O<br />
NH + 2<br />
250 nmole <br />
1.0 µmole <br />
O<br />
Absorbance and Fluorescence Emission<br />
492 nm 517 nm<br />
400 450 500 550 600 650<br />
Wavelength (nm)<br />
* HPLC purification required<br />
Absorbance<br />
Fluorescence Emission<br />
HN<br />
O<br />
O –<br />
45
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