Fluorophores - Integrated DNA Technologies

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Table of Contents 1 General Information General Information 1 IDT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Inside Front Cover How to Order . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Inside Front Cover Shipping Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Inside Front Cover About IDT . . . . 1 IDT Advantage 4 2DNA Synthesis and Purification Custom Oligonucleotide Synthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Yield Guarantees . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Oligonucleotide Purification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Custom DNA Synthesis in Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Preparative and Analytical Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 3RNA Synthesis and Purification Custom RNA Oligonucleotide Synthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Purification & Yield Guarantees . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 RNA Modifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 RNase Substrates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 4Functional Genomics/Gene Knockdown Antisense Oligonucleotides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 RNA Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 DNA-Directed RNAi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 miRNA . . . . . 77 5DNA & RNA Probes for Fluorescence-Based Applications Dual-Labeled DNA Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 Dual-Labeled LNA Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 Dual-Labeled RNA Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 Molecular Beacons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 Phone: 03-5248-3066 Fax: 03-5248-2930 e-mail: oligo@mbl.co.jp 2
Table of Contents 6 Modifications Phosphorylation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Attachment Chemistry / Linkers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Fluorophores . 45 Dark Quenchers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 Spacers . . . . . 62 Modified Bases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 Phosphorothioate Bonds . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 1 General Information 7Molecular Biology Products RNaseAlert Substrate and Nuclease Detection System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 DNaseAlert Substrate and Nuclease Detection System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72 Nuclease-free Buffers and Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 StarFire® Nucleic Acid Labeling System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 ReadyMade Primers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76 miRNA Cloning Linker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77 Oligo Length Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 Microarray Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 SpotQC . . . . . 80 Oligo Spotting Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 8Custom Services SciTools (PrimerQuest , mFold & OligoAnalyzer) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82 Packaging and Formulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 Mass Spectrometry and CE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 Preparative and Analytical Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 9Usage, Warranty and Licenses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 10 3
Page 2 and 3: 1 General Information
Page 6 and 7: The IDT Advantage 1 General Informa
Page 8 and 9: Custom DNA Synthesis Synthesis Scal
Page 10: Custom DNA Synthesis in Plates
Page 13 and 14: Custom RNA Synthesis and Purificati
Page 15 and 16: Functional Genomics Antisens
Page 17 and 18: A n t i s e n s e O l i g o n u c l
Page 19 and 20: 4 Functional Genomincs
Page 21 and 22: RNA Interference 4 Functional Genom
Page 23 and 24: Purity and QC Duplex Yie
Page 25 and 26: 4 Functional Genomincs
Page 27 and 28: DNA-Directed RNA Interference (ddRN
Page 29 and 30: 4 Functional Genomincs 27
Page 31 and 32: Fluorescent DNA and RNA Probes 5 DN
Page 33 and 34: Dual-Labeled DNA Probes Reporte
Page 35 and 36: Reporter: 5' CAL Fluor ® Red 610 Q
Page 37 and 38: Quencher: 5' Iowa Black RQ Reporte
Page 39 and 40: Dual-Labeled RNA Probes - Only at I
Page 41 and 42: Modifications Technical Note!
Page 43 and 44: Attachment Chemistry / Linkers Amin
Page 45 and 46: Attachment Chemistry / Linkers Biot
Page 47 and 48: Attachment Chemistry / Linkers Thio
Page 49 and 50: Fluorophores 5' MW: 883.9 Int. MW:
Page 51 and 52: Fluorophores Bodipy® Dyes Bodipy
Page 53 and 54: Fluorophores Cy Dyes Cy3 * 5'
Page 55 and 56:
Fluorophores Fluorescein dT * MW: 8
Page 57 and 58:
Fluorophores 5' MW: 673.5 Int. MW:
Page 59 and 60:
Fluorophores Rhodamine Red -X NHS
Page 61 and 62:
Fluorophores TET Ab max 522 nm Em
Page 63 and 64:
Dark Quenchers Black Hole Quencher
Page 65 and 66:
Spacers Spacer 18 MW: 344.3 SCALE
Page 67 and 68:
Modified Bases Inverted dT MW: 304.
Page 69 and 70:
Modified Bases 5-Nitroindole MW: 34
Page 71 and 72:
Modified Bases Iso-dG* 5' MW: 329.2
Page 73 and 74:
RNaseAlert Substrate and Nuclease
Page 75 and 76:
Nuclease-free Buffers & Reagents 7
Page 77 and 78:
® Nucleic Acid Labeling System ®
Page 79 and 80:
miRNA Cloning Linker
Page 81 and 82:
Microarray-Related Products DNA
Page 83 and 84:
Custom Services 8 Custom Services 8
Page 85 and 86:
Packaging and Formulation
Page 87 and 88:
Service Pricing Lab-Ready O
Page 89 and 90:
Usage, Warranty and Licenses Usage
Page 91 and 92:
10 Index Index General Information
Page 93 and 94:
10 Index Index ReadyMade Primers, c
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Fluorophores - Integrated DNA Technologies

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