MCO-19AIC MCO-19AICUV MCO-19AICUVH Series - Panasonic ...

Operating Instructions 

 

 

 

CO 2 Incubator 

 

 

 

Please read these instructions carefully before using this product, and save this manual for future use. 

See page 66 for all Model Nos.

CONTENTS 

INTRODUCTION P. 2 

PRECAUTIONS FOR SAFE OPERATION P. 3 

LABELS ON INCUBATORS P. 7 

ENVIRONMENTAL CONDITIONS P. 7 

INCUBATOR COMPONENTS P. 8 

Control panel and keypad P. 10 

Remote alarm terminal P. 11 

INSTALLATION SITE P. 12 

INSTALLATION P. 13 

Connecting a CO 2 gas cylinder P. 14 

PREVENTING CONTAMINATION P. 15 

PRECAUTIONS FOR CULTURES P. 16 

Using unlock key P. 16 

INITIAL CLEANING METHOD P. 17 

REMOVING INNER ATTACHMENTS P. 18 

INSTALLING INNER ATTACHMENTS P. 20 

FILLING HUMIDIFYING PAN P. 21 

WATER LEVEL SENSOR P. 22 

ROUTINE MAINTENANCE P. 23 

CORRECT OPERATION P. 23 

LCD PANEL P. 24 

BASIC OPERATIONS ON CONTROL PANEL P. 25 

BASIC PARAMETERS 

Setting chamber temperature and CO 2 density P. 26 

Setting key lock P. 27 

Removing key lock P. 27 

Setting key lock password P. 28 

Setting high limit alarm temperature P. 30 

ALARM PARAMETERS P. 31 

UV LAMP PARAMETERS 

Using UV lamp P. 32 

Precautions when using UV lamp P. 33 

Setting UV lamp ON period P. 34 

Lighting UV lamp for 24 hours P. 35 

OTHER PARAMETERS 

Setting date, time, and log interval P. 36 

Initial settings (LCD/DAQ parameters) P. 38 

DISPLAYING THE LOG P. 39 

Transferring data P. 40 

H 2 O 2 DECONTAMINATION P. 41 

Precautions when handling H 2 O 2 reagent P. 45 

ALARMS, SAFETY, AND SELF-DIAGNOSIS P. 46 

CALIBRATION 

Temperature/CO 2 calibration P. 49 

TROUBLESHOOTING P. 51 

DISPOSAL OF UNIT P. 53 

AUTOMATIC CO 2 CYLINDER CHANGEOVER P. 58 

AUTOMATIC CO 2 DENSITY CALIBRATION P. 60 

STACKING INCUBATORS P. 63 

SPECIFICATIONS P. 65 

PERFORMANCE P. 66 

SAFETY CHECK SHEET P. 67 

1

INTRODUCTION 

■ Read this operating instruction carefully before using the Product and follow the instructions for safety 

operation. 

■ Our company disavows any responsibility for safety if the Product is used for other than the intended 

use or used with any procedures other than those given in this operating instruction. 

■ Keep this operating instruction in a suitable place so that it can be referred to as necessary. 

■ The contents of this operating instruction are subject to change without notice for improvement of 

performance or functions. 

■ Contact our sales representative or agent if any page of the operating instruction is lost or the page 

order is incorrect. 

■ Contact our sales representative or agent if any point in this operating instruction is unclear or if there 

are any inaccuracies. 

■ No part of this operating instruction may be reproduced in any form without the expressed written 

permission of our company. 

CAUTION 

Our company guarantees the product under certain warranty conditions. Our company in no 

way shall be responsible for any loss of content or damage of content. 

Microsoft, Windows, Windows 7, Windows Vista, Windows XP and Windows 2000 are registered 

trademarks in the United States of Microsoft Corporation and various other countries. 

2

PRECAUTIONS FOR SAFE OPERATION 

It is imperative that the user complies with this operating instruction 

as it contains important safety advice. 

Items and procedures are described so that you can use this unit correctly and safely. 

If the precautions advised are followed, this will prevent possible injury to the user and 

any other person. 

Precautions are illustrated in the following way: 

WARNING 

Failure to observe WARNING signs could result in a hazard to personnel 

possibly resulting in serious injury or death. 

CAUTION 

Failure to observe CAUTION signs could result in injury to personnel and 

damage to the unit and associated property. 

Symbol shows; 

this symbol means caution. 

this symbol means an action is prohibited. 

this symbol means an instruction must be followed. 

Be sure to keep this operating instruction in a place accessible to users of this unit. 

< Label on the unit > 

This mark is labeled on the cover in which the electrical components of high voltage 

are enclosed to prevent the electric shock. 

The cover should be removed by a qualified engineer or a service personnel only. 

WARNING 

As with any equipment that uses CO 2 gas, there is a likelihood of oxygen depletion in the vicinity 

of the equipment. It is important that you assess the work site to ensure there is suitable and 

sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a 

safe environment must be considered. These may include atmosphere monitoring and 

warning devices. 

3


WARNING 

Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to 

rain water. 

Only qualified engineers or service personnel should install the unit. The installation by 

unqualified personnel may cause electric shock or fire. 

Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from 

turning over. If the floor is not strong enough or the installation site is not adequate, this may result 

in injury from the unit falling or tipping over. 

Never install the unit in a humid place or a place where it is likely to be splashed by water. 

Deterioration of the insulation may result which could cause current leakage or electric shock. 

Never install the unit in a flammable or volatile location. This may cause explosion or fire. 

Never install the unit where acid or corrosive gases are present as current leakage or electric 

shock may result due to corrosion. 

Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not 

grounded, it will be necessary to install a ground by qualified engineers. 

Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such 

grounding may cause electric shock in the case of an incomplete circuit. 

Connect the unit to a power source as indicated on the rating label attached to the unit. Use 

of any other voltage or frequency other than that on the rating label may cause fire or electric shock. 

Never store volatile or flammable substances in this unit if the container cannot be sealed. These 

may cause explosion or fire. 

Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit. 

This may cause electric shock or injury by accidental contact with moving parts. 

Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use 

may cause bad effect on your health or environment. 

Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any 

repair or maintenance of the unit in order to prevent electric shock or injury. 

Do not touch any electrical parts (such as power supply plug) or operate switches with a wet 

hand. This may cause electric shock. 

4

WARNING 

Ensure you do not inhale or consume medication or aerosols from around the unit at the time of 

maintenance. These may be harmful to your health. 

Never splash water directly onto the unit as this may cause electric shock or short circuit. 

Never put containers with liquid on the unit as this may cause electric shock or short circuit when 

the liquid is spilled. 

Never bind, process, or step on the power supply cord, or never damage or break the power 

supply plug. A broken supply cord or plug may cause fire or electric shock. 

Do not use the supply cord if its plug is loose. Such supply cord may cause fire or electric shock. 

Never disassemble, repair, or modify the unit yourself. Any such work carried out by an 

unauthorized person may result in fire, or electric shock or injury due to a malfunction. 

Disconnect the power supply plug if there is something wrong with the unit. Continued 

abnormal operation may cause electric shock or fire. 

When removing the plug from the power supply outlet, grip the power supply plug, not the cord. 

Pulling the cord may result in electric shock or fire by short circuit. 

Disconnect the power supply plug before moving the unit. Take care not to damage the power 

cord. A damaged cord may cause electric shock or fire. 

Disconnect the power plug when the unit is not used for long periods. Keeping the connection 

may cause electric shock, current leakage, or fire due to the deterioration of insulation. 

If the unit is to be stored unused in an unsupervised area for an extended period, ensure that 

children do not have access and that doors cannot be closed completely. 

The disposal of the unit should be accomplished by appropriate personnel. Remove doors to 

prevent accidents such as suffocation. 

Do not put the packing plastic bag within reach of children as suffocation may result. 

Use the reagent specified by our company for H 2 O 2 decontamination. Using a different H 2 O 2 

solution may result in explosion or damage to the incubator. 

When performing H 2 O 2 decontamination, securely close the internal and external doors. Failure to 

do so may be harmful to health due to leakage of H 2 O 2 gas. 

During H 2 O 2 decontamination, plug the access hole with the silicon cap that is provided. Failure 

to do so may be harmful to health due to leakage of H 2 O 2 gas. 

5


CAUTION 

This unit must be plugged into a dedicated circuit protected by branch circuit breaker. 

Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap 

may cause fire resulting from abnormal heating. 

Never store corrosive substances such as acid or alkali in this unit if the container cannot be 

sealed. These may cause corrosion of inner components or electric parts. 

Check the setting when starting up of operation after power failure or turning off of power 

switch. The stored items may be damaged due to the change of setting. 

Be careful not to tip over the unit during movement to prevent damage or injury. 

Prepare a safety check sheet (copy the last page) when you request any repair or maintenance for 

the safety of service personnel. 

Wear rubber gloves when handling the H 2 O 2 reagent. Direct contact with the H 2 O 2 reagent may 

result in inflammation of the skin. 

H 2 O 2 decontamination can be performed only for the chamber and chamber attachments with 

standard specifications, and not for any other objects. 

Perform H 2 O 2 decontamination with the chamber attachments arranged as specified by our 

company. Arranging them in a different way may result in insufficient decontamination. 

After H 2 O 2 decontamination has been completed, wear rubber gloves and use a non-woven cloth 

to wipe off the residual H 2 O 2 fluid from the bottom of the chamber, any objects that were 

decontaminated, and the bottoms of ducts. 

6

LABELS ON INCUBATOR 

Warning and caution labels are attached to the incubator. The following table describes the labels. 

This label is attached to covers that access high-voltage electrical components to 

prevent electric shock. Only a qualified engineer or service personnel should be 

allowed to open these covers. 

This symbol indicates an ultraviolet light (UV) caution. 

This symbol indicates that caution is required. Refer to product documentation for 

details. 

This symbol indicates a hot surface. 

This symbol indicates an earth. 

This symbol means “ON” for a power switch. 

This symbol means “OFF” for a power switch. 

ENVIRONMENTAL CONDITIONS 

This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1): 

■ Indoor use; 

■ Altitude up to 2000 m; 

■ Ambient temperature 5 o C to 40 o C 

■ Maximum relative humidity 80 % for temperature up to 31 o C decreasing linearly to 50 % relative 

humidity at 40 o C; 

■ Mains supply voltage fluctuations not to exceed ±10 % of the nominal voltage; 

■ Other supply voltage fluctuations as stated by the manufacturer; 

■ Transient overvoltages according to Installation Categories (Overvoltage Categories) II; For mains 

supply the minimum and normal category is II; 

■ Pollution degree 2 in accordance with IEC 60664. 

7

INCUBATOR COMPONENTS 

Handles 

6 5 12 7 9 (inside) 8 

2 

1 

4 

15, 16 (inside) 

For MCO-19AIC(UVH)/19AIC(UV) or 

when MCO-19UVS is installed. 

Handles 

22 

10 

13 (inside) 

14 

3 

21 

18 

17 

20 

11 

19 

For MCO-19AIC(UVH)/19AIC(UV) or 

when MCO-19UVS is installed. 

Rear Right Side 

Rear Left Side 

8

1. Outer door: The outer door is held to the frame with the magnetic seal. The door heater is installed in 

the door panel. The door opening is reversible. Contact our sales representative or agent to change the 

door hinge from left to right or vice versa. 

2. Inner door: The inner door is made of tempered glass. However do not subject the glass to excessive 

impacts. 

3. Leveling feet: The leveling feet can be turned to adjust the height. Adjust the feet so that the incubator 

is level. 

4. Trays: The trays can be pulled out toward you. 

5. Tray supports: The tray supports can be removed by lifting the front side and pulling toward you. 

6. Side supports: The right and left side supports can be removed for disinfection. Refer to page 19 and 

23. 

7. Fan cover: The fan cover serves as the inlet for circulating air. It is removable. 

8. Duct: The duct for the path for circulating air. It is removable. 

9. Fan (inside the duct): The fan is made from polypropylene resin. It can be disinfected in an autoclave. 

10. Sample air outlet: The sample air outlet also functions as an internal gas outlet. Normally, cover this 

outlet with the sample air outlet cap. 

11. Connecting port A/B for CO 2 gas pipe: When the optional MCO-21GC gas auto changer is installed, 

both ports A and B are available. If the MCO-21GC is not installed, only port A is available. Refer to page 

14 for gas cylinder connection. Ensure that the gas pressure is set at 0.03 MPa(G) (0.3 kgf/cm 2 (G), 4.3 

psi(G)). Refer to page 58 for gas auto changer. 

12. Door switch: Detects the door opening/closing and stops the fan and electromagnetic valve for CO 2 

when the door is open. The UV lamp* is also deactivated by the door opening*. 

13. Humidifying pan: Fill the humidifying pan with sterile distilled water. Install the humidifying pan 

properly so that it can be covered with the humidifying pan cover. 

14. Humidifying pan cover: The cover prevents the UV light entering the chamber. When filling the 

humidifying pan, lift the front side and take out the humidifying pan. Refer to page 21 for details. 

15. UV lamp*: This UV lamp does not generate ozone. Never look directly at the UV light. For 

replacement, contact our sales representative or agent. 

16. Water level sensor: This sensor detects the water level in the humidifying pan. Refer to page 22 for 

details. 

17. Remote alarm terminals: Refer to page 11. 

18. Access port: Place the silicon caps on the port both outside and inside when the port is not being 

used. 

19. Power switch: This is the main switch for the incubator. It also functions as an overcurrent breaker. 

20. Glow starter*: The glow is started if for the UV lamp. 

21. Attachment location for electric key: This is the attachment location for the electric key included in 

the optional component H 2 O 2 decon set (MCO-HL). This kit must be attached to perform H 2 O 2 

decontamination. Refer to the installation procedure for MCO-HL for details. 

22. Sample air outlet cap: Always attach this cap except when using the sample air outlet. 

* MCO-19AIC(UVH)/19AIC(UV) or when an optional UV system set (MCO-19UVS) is installed. 

9

INCUBATOR COMPONENTS 

Control panel and keypad 

1 

3 

2 

5 

10 

11 

4 

8 

9 

6 7 

1. LCD panel 

2. High limit regulator: 

This regulator is used to set the high 

temperature limit. 

3. Overheat indicator (OVER HEAT): 

This indicator lights when the chamber 

temperature reaches the high limit. 

4. Menu key (MENU): 

Press this key to access the menu. 

7. CE Key (CE): 

Press this key to clear the entered value 

when entering a setting. 

8. Cursor Keys (Up, Down, Left, and Right): 

Use these keys to move the cursor on the 

LCD panel. 

9. Enter key (ENT): 

Press this key to select a menu command. 

When entering a setting, pressing this key to 

move to the next parameter. 

5. Contrast knob (CONT.): 

Turn this knob to adjust the contrast of the 

LCD. 

6. Buzzer key (BUZZER): 

Press this key to silence the buzzer. 

Note: It is not possible to silence the buzzer 

for the high limit temperature alarm. 

10. Numeric Keys 

11. H 2 O 2 Key (H2O2): 

Press this key to start the H 2 O 2 

decontamination. Refer to page 41-44 for 

details. 

※ The following optional accessories must be installed to perform decontamination. 

・UV system set (MCO-19UVS) 

(This is provided as standard equipment for the MCO-19AIC(UVH)/19AIC(UV).) 

・H 2 O 2 decon set (MCO-HL) 

・H 2 O 2 generator (MCO-HP) 

10

Remote alarm terminal 

The remote alarm terminal is located at the rear right side of the incubator. 

Remote alarm 

terminals 

The alarm is outputted from this terminal. Contact capacity is DC 30 V, 2 A. 

Normal: 

Open 

Alarm: 

Closed 

Note: 

• When the power switch is OFF or power is interrupted, the contact output will be closed. 

• The remote alarm cannot be silenced by pressing the buzzer key (BUZZER) since the remote alarm is 

not conjunct with the buzzer key (BUZZER). 

REMOTE ALARM 

MAX DC30V 2A 

Remote alarm terminal 

N.O. 

11

INSTALLATION SITE 

For correct operation of the incubator, install it in a location with the following conditions. 

WARNING 

When using CO 2 gas for control, make sure that there is adequate ventilation. Using CO 2 gas in a 

small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition, 

when opening the incubator doors, do not directly inhale the air in the chamber. 

Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO 2 de l’air peut s’élever et peut 

être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la 

porte. 

• Normal air environment 

Install the incubator in an environment with normal air. 

• Do not expose to direct sunlight 

Do not install the incubator in a location where it will be exposed to direct sunlight. If the incubator is 

operated in direct sunlight, performance will be adversely affected. 

• Separate from heat sources 

Do not install the incubator near significant heat sources, such as heaters, boilers, ovens, or autoclaves. 

Heat will adversely affect the performance of the incubator. 

• Ambient temperature at least 5 C lower than set temperature 

The control temperature of the incubator is at least 5 C higher than the ambient temperature. For 

example, if the chamber is controlled at 37 C, the ambient temperature must normally be no more than 

32 C. Do not allow the ambient temperature to become too high. 

• Strong and level floor 

Select a site with a strong and level floor. If the floor is uneven or the installation is not level, the incubator 

will be unstable and this may cause accident or injury. To avoid vibration and noise, always make sure 

that the installation is stable. An unstable surface may result in vibration or noise. 

WARNING 

Install the incubator at a location that can support the weight. If the floor is not strong enough or if the 

installation is insufficient, the incubator may fall over and cause injury. 

Always make sure that the floor is strong, even, and level, and that the incubator will not tip over. 

An insufficient installation may result in injury due to water leakage or the incubator falling over. 

• Low humidity 

Select a site with a relative humidity of 80 %R.H. or lower. Using the incubator in high humidity may result 

in current leakage or electric shock. 

WARNING 

Do not use the incubator outdoors. If the incubator is exposed to rain water, it may result in current 

leakage or electric shock. 

Never install the incubator in a moist location, such as near a sink or water line, or where it is 

likely to be exposed to water. In addition, do not install it near water or steam pipes. Moisture can 

cause the insulation to deteriorate, which may result in current leakage or electric shock. 

• No inflammable or corrosive gas 

Never install the incubator in a location where it will be exposed to inflammable or corrosive gas. Doing so 

may result in explosion or fire. In addition, insulation may deteriorate due to corrosion of protective casing, 

resulting in current leakage or electric shock. 

• No falling objects 

Do not install the incubator in a location where there is the possibility of objects falling from above. Doing 

so may result in damage or accident. 

12

INSTALLATION 

1. Remove the packing tape and clean up. 

Remove all the tape that is securing the doors and the inner attachments. Open the doors for ventilation. 

If the outer panels are dirty, dampen a cloth with a diluted neutral detergent and wipe them. (Using an 

undiluted solution may damage the plastic. Follow the diluting instructions for the detergent that is used.) 

Wipe off the residual detergent with a wet cloth and then wipe off any moisture. 

Note: 

Remove the cable tie banding the power supply cord. Prolonged banding may cause the corrosion of 

the cord coating. 

WARNING 

Do not leave the plastic wrapping bags within reach of children. If the bag is placed over a child’s 

head, it can block the mouth and nose and cause suffocation. 

2. Adjust the leveling feet. 

Adjust the leveling feet by turning them counterclockwise to level 

the incubator. (See the illustration on the right.) 

3. Ground the incubator. 

Ground the incubator during installation to prevent electric shock 

in case the insulation is not sufficient. If there is no ground wire at 

the location, consult with qualified service personnel. 

• When a ground must be installed 

If a grounded 3-pole outlet is not available, then a ground must 

be installed. Consult with qualified service personnel. 

Leveling feet 

WARNING 

To prevent electric shock, always ground the incubator. If grounding is not possible, then have a ground 

installed by qualified personnel. If the incubator is not grounded, it may result in electric shock. 

Never connect the ground wire to a gas pipe, water pipe, lightning rod, or telephone ground wire. 

Doing so may cause electric shock. 

• Installing a ground fault circuit breaker 

If using the incubator in the location with moisture or humidity cannot be avoided, then it is recommended 

that a ground fault circuit breaker be installed in the power supply circuit (i.e., the power supply at the 

incubator). Have the circuit breaker installed by qualified service personnel. 

CAUTION 

Do not climb on the incubator or place objects on top of it. Doing so may damage it or cause it to fall 

over, resulting in injury. If it is to be stacked, refer to page 63 and stack it securely. 

• When the incubator is not in use 

Empty the water from the humidifying pan and remove moisture from the chamber. Make sure that the 

chamber is completely dry before closing the doors. Failure to do so may result in damage. 

• Before moving the incubator 

Before moving the incubator, empty the water from the humidifying pan, disconnect the power supply plug 

from the outlet, and make sure that the cord will not be damaged. Failure to do so may result in electric 

shock or fire. 

13

INSTALLATION 

Connecting a CO 2 gas cylinder 

WARNING 

When connecting a gas cylinder to the incubator, confirm the gas type. Confirm that the connections 

are secure and that no gas will escape. Be sure to use the specified pressure. Using an incorrect 

gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation due to escaping 

gas. 

Install the incubator in a location with adequate ventilation. If adequate ventilation cannot be 

provided, then install an alarm system using CO 2 and O 2 densitometers. 

1. Use a liquefied CO 2 gas cylinder (at least 99.5 % pure). The siphon (dip tube) type cannot be used. 

2. Install a gas regulator rated at 25 MPa(G) (250 kgf/cm 2 (G), 3600 psi(G)) for the primary side, and 0.2 

MPa(G) (2 kgf/cm 2 (G), 30 psi(G)) for the secondary side. 

3. Using the gas tube that is provided, connect the gas regulator to the connecting port A/B for CO 2 gas 

pipe at the rear left side of the incubator. 

Note: 

If the CO 2 gas is supplied to multiple CO 2 incubators from a single gas cylinder, a CO 2 solid will be 

formed in the gas regulator. The gas regulator safety valve will operate, and there may an explosive 

sound. 

When the MCO-21GC is not mounted 

Using the gas tube that is provided, connect the gas regulator for the CO 2 gas cylinder to connecting port 

A for CO 2 gas pipe located at the rear side of the incubator. After connecting the gas tube, make sure that 

no gas is leaking. 

When the MCO-21GC is mounted 

Connect a pair of CO 2 gas cylinders with gas regulators independently. The gas supply line will be 

switched automatically. Connect the main cylinder to the connecting port A for CO 2 gas pipe and the 

reserve cylinder to the connecting port B for CO 2 gas pipe. After connecting the cylinders, make sure that 

no gas is leaking. 

• For details on installing the optional automatic CO 2 cylinder changeover kit (MCO-21GC), refer to the 

MCO-21GC installation guide. For details on using the MCO-21GC, refer to page 58. 

4. Set the CO 2 gas on the secondary side to 0.03 MPa(G) (0.3 kgf/cm 2 (G), 4.3 psi(G)) for gas injection. As 

the pressure increases, the CO 2 gas density control range will increase. Excessive pressure may cause 

gas supply lines inside the incubator to come loose, which may result in gas poisoning or oxygen 

deprivation due to the escaping of gas. If gas lines come loose, the incubator must be repaired. 

• The gas lines connected to the incubator will degrade over time. If any deterioration or abnormalities 

are found during inspection, replace the lines immediately. 

14

PREVENTING CONTAMINATION 

To prevent contamination of the chamber, select a suitable installation site. 

• Avoid locations with high temperatures or humidity. 

Avoid locations with high temperatures or humidity, because of a greater presence of microorganisms in 

the air. 

• Avoid locations with passers-by or drafts. 

Avoid locations near doors, air conditioners, fans, etc., where passers-by or drafts can facilitate the entry 

of microorganisms into the chamber. 

• If possible, use a cleanroom. 

To achieve a better culture, it is recommended that a cleanroom be used if one is available. 

• Use clean containers. 

The greatest cause of contamination is dirty containers for cultures. Be careful not to get containers or 

trays dirty when taking them in and out. 

• Keep the chamber clean. 

Wipe off any fingerprints. If water spills from the humidifying pan, or if the doors are left open for a long 

time, condensation may form on the inside of the doors. If that occurs, wipe off the condensation with a 

dry sterile gauze. In particular, clean and disinfect the chamber if the culture medium is spilled. For details, 

refer to “ROUTINE MAINTENANCE” on page 23. 

• Use sterile distilled water in the humidifying pan. 

Always use sterile distilled water in the humidifying pan. Do not use ultrapure water, because it may 

cause red rust-like particles in the humidifying pan. 

The water level alarm is displayed in the status display area on the control panel. Quickly refill the water 

in the humidifying pan when the water level alarm is displayed. Adding low-temperature water will 

significantly lower the temperature in the chamber. Clean the humidifying pan once a month. 

• Keep the incubator out of direct airflows from air conditioners or fans. 

Cool airflow from an air conditioner may cause condensation and lead to possible contamination. 

15

PRECAUTIONS FOR CULTURES 

• Leave space between culture containers. 

Always leave space for ventilation between culture containers (Petri dishes, flasks, etc.). Inadequate 

spacing may result in uneven temperature distribution and CO 2 gas density. 

• Do not place harmful materials in the chamber. 

Never place samples that release acidic, alkali, or corrosive gas in the chamber. Doing so may cause 

damage resulting from discoloration or corrosion. 

• Close the inner door. 

Always close the inner door before closing the outer door. Failure to close the inner door will adversely 

affect performance even if the outer door is closed. 

• Open and close the doors gently. 

Always open and close the doors gently. Closing the doors forcefully may cause spillage of the culture 

medium, incomplete closing, or damage to the gasket. Before opening the inner door, check through the 

glass to confirm that the UV lamp is OFF (if the MCO-19AIC(UVH)/19AIC(UV) or the optional 

MCO-19UVS is installed). 

• Be careful when closing the outer door. 

Use the handle when closing the outer door. Holding the door in other places may cause injury by getting 

fingers caught in the door. Do not lean on the outer door. Doing so may result in injury from the outer door 

coming loose or the incubator falling over, or it may cause current leakage or electric shock. 

• Be careful of the inside of the outer door. 

The inside of the outer door may become hot. 

• Avoid using excessive force on the inner door. 

Do not put your hand on the glass, poke it with sharp objects, or apply strong force. Doing so may result 

in injury from breaking the glass. 

• Check the cause of any alarm buzzer. 

If an alarm buzzer sounds while the incubator is in use, immediately check the cause of the alarm. For 

details on what may cause an alarm buzzer to sound, refer to page 46. 

Using unlock key 

• Unlocking when power is interrupted 

If power is interrupted to the MCO-19AIC(UVH) or MCO-19AIC(UV)/19AIC with an MCO-HL installed, the 

outer door is electrically locked. To unlock the outer door while the power is interrupted, use the unlock 

key that is provided. To lock the outer door again, turn the unlock key to the lock direction while the outer 

door is open. After the outer door has been locked condition manually, then close the outer door. 

Note: 

The outer door cannot be locked by using the unlock key while the outer door is closed. Lock the outer 

door while it is open. Attempting to turn the unlock key while the outer door is closed may damage the 

electric lock system. 

WARNING 

For the MCO-19AIC(UVH) or MCO-19AIC(UV)/19AIC with an MCO-HL installed, the outer door is 

electrically locked during decontamination. The chamber is filled with H 2 O 2 gas during decontamination. 

Do not unlock the outer door during decontamination. 

16

INITIAL CLEANING METHOD 

Before using the incubator for the first time, clean dirt (tape residue, smear, etc.) from the 

chamber and the inner attachments thoroughly. To keep the chamber clean is essential to 

get the proper performance out of the incubator. Use the following steps to clean the 

incubator properly. 

1. Remove the inner attachments, referring to “REMOVING INNER ATTACHMENTS” on page 18. 

2. Clean the removed inner attachments, the chamber inside walls and the inner door gaskets with a cloth 

or sponge soaked in neutral detergent, diluted by 5 % or less. (Undiluted detergent can damage the 

plastic components. For the dilution, refer to the instruction of the detergent.) (Fig. 1) 

CAUTION 

Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause 

discoloration, corrosion, or rusting. 

Be careful to keep the diluted detergent or water out of the temperature sensor, the CO 2 gas injection port, 

the inner sample air access port, the fan motor shaft bearing, and the inner sample air outlet 

(Fig.2 ). Also, do not wash the temperature sensor, the water level sensor and the UV lamp using 

detergent. Doing so may cause failure. (Fig. 2) 

3. Soak a gauze or unwoven cloth in distilled water and wring it tightly, and then wipe off the residual 

detergent thoroughly. 

4. Wash the silicon caps (2 pcs) for the access port and the fan using the above mentioned detergent and 

rinse them with distilled water, and then autoclave them for sterilization (121 o C, 20 minutes). 

5. Wipe up the inside walls and the inner attachments like trays thoroughly with a cloth or unwoven cloth 

soaked in alcohol for disinfection. Be careful not to leave any residue alcohol. 

6. Reinstall the inner attachments correctly and securely, referring to “INSTALLING INNER 

ATTACHMENTS” on page 20. 

Silicon 

cap 

Temp. sensor 

(UV lamp) 

Water level sensor 

Fig. 1 Fig. 2 

17

REMOVING INNER ATTACHMENTS 

CAUTION 

Wear rubber gloves when performing maintenance on the chamber. Failure to wear gloves may 

result in cuts or abrasions from sharp edges or corners. 

Be careful not to damage the water level sensor or the UV lamp in the duct (if the 

MCO-19AIC(UVH)/19AIC(UV) or the optional MCO-19UVS is installed). 

Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause 

discoloration, corrosion, or rusting. 

1. Turn OFF the power to the incubator. 

2. Open the outer and inner doors and pull out all the trays. (Fig. 1) 

3. Lift the front of the tray supports and pull them out. (Fig. 2) 

4. Lift the humidifying pan cover off from the pins on the rear side. (Fig. 3) 

5. Pull out the humidifying pan. (Fig. 4) 

6. Loosen the two screws securing the fan cover and take off the fan cover. (Fig. 5) 

7. Lift the duct and remove it from the pins on the rear side. (Fig. 6) 

Pins 

Tray support 

Humidifying pan cover 

Fig. 1 Fig. 2 Fig. 3 

Duct 

Fan cover 

Humidifying pan 


18

8. Remove the fan by pulling out the central spring and then by pulling out the fan. (Fig. 7) 

9. Remove the screw securing the clamp for the side support. (Fig. 8) 

10. Remove the clamp. (Fig. 9) 

11. Lift the side supports off of the pins. (Fig. 10) 

12. Remove the silicon caps of the access port, one each from interior (Fig. 11) and exterior (Fig. 12). 

1Pull out the central spring 

Screw 

Clamp 

2Pull out the fan 


Silicon cap 

Silicon cap 

Side 

support 

Fig. 10 Fig. 11 Fig. 12 

19

INSTALLING INNER ATTACHMENTS 

Use the following steps to install the inner attachments properly. 

1. To reinstall all the attachments, perform the procedure in reverse order from step 12 on page 19. 

2. When installing the fan, insert it to the motor shaft securely. Lightly turn the fan manually to make sure 

that it does not touch the rear panel. Then install the central spring. (Fig. 1) 

CAUTION 

If the fan is not inserted deep enough, the intended velocity performance cannot be achieved, which may 

cause culture failure or insufficient decontamination. 

3. To install the duct, confirm 4 pins are securely installed in the 4 holes of the duct. (Fig. 2) 

CAUTION 

If the duct is fixed insecurely, the intended velocity performance cannot be achieved, which may cause 

culture failure or insufficient decontamination. 

1 Push the fan deeply. 

2 Install the central spring afterwards. 

3 Lightly turn the fan manually to make 

sure that it does not touch the rear panel. 

Fig. 1 Fig. 2 

4. To install the side support, confirm the direction of fixing holes as shown in Fig. 3. 

5. As shown in Fig. 4, set the tray with only the front edge bent down. If the tray is set in the wrong 

direction, it may not be level and may become unstable. 

Fig. 3 

Bent down 

Fig. 4 

20

FILLING HUMIDIFYING PAN 

Use the following procedure to fill the humidifying pan or to replace the water. 

1. Lift the humidifying pan cover. (Fig. 1) 

2. Pull out the humidifying pan toward you. (Fig. 2) 

3. Dispose of the remaining water in the humidifying 

pan and clean the humidifying pan with a diluted 

detergent. Then rinse it thoroughly with distilled 

water and wipe it with alcohol for disinfection. 

4. Wipe all moisture from the bottom of the chamber. 

Fig. 1 

5. Return the humidifying pan to the chamber and 

pour sterile distilled water (approx. 1.5 L, preheated 

to 37 C). (Fig. 3) 

CAUTION 

Install the humidifying pan in a longitudinal direction 

as its shorter side is placed in the back. Improper 

installation may cause condensations. 

Fig. 2 

6. Set the humidifying pan with the inner side flush 

against the back, and replace the humidifying pan 

cover. Close the inner door and the outer door, and 

confirm that RH PAN is not displayed in reverse 

video in the status display field. 

Note: 

• Preheat the water to 37 C to be poured into the 

humidifying pan. Adding low-temperature water will 

lower the temperature and humidity in the chamber. 

• If the water level alarm indication “RH PAN” is 

displayed, use the above procedure to replenish the 

water. 

Fig. 3 

CAUTION 

When refilling the water in the humidifying pan, always wipe off any dirt from the water level sensor 

with alcohol for disinfection. While doing that, be careful not to apply excessive force to the sensor 

lead wire. 

Caution: 

In order to adjust the humidity inside the incubator chamber optimally and prevent condensation on the 

chamber surface and the inner door, it is designed to create the lower temperature area underneath the 

humidifying pan to recondense the evaporated moisture in the humidifying pan. Although the 

condensation may be seen round the humidifying pan on the bottom of chamber (inside the humidifying 

pan cover), it is not a malfunction. 

21

WATER LEVEL SENSOR 

This incubator is equipped with a water level sensor. The water level sensor is set automatically when the 

humidifying pan is installed. Take care not to damage the water level sensor when removing or installing 

the humidifying pan. 

When the humidifying pan is removed (side view) 



When the humidifying pan is installed (side view) 

Note: Make sure that the humidifying pan is pushed all the way to the back and that the water level 

sensor comes down when you install the humidifying pan. 

Note: 

• Lift the water level sensor before installing the humidifying pan if the water level sensor is in the lower 

position after maintenance. 

• When installing the humidifying pan, make sure that the humidifying pan is set properly and that water 

level sensor comes down into the humidifying pan. “RH PAN” will be displayed in reverse video in the 

status display field on LCD panel if the water level sensor does not come down completely. If necessary, 

set the humidifying pan again in the proper location. 

• The water level sensor detects the water level every 30 minutes and just after the outer door is closed. It 

takes several seconds to detect the water level. Therefore, “RH PAN” may be displayed in reverse video 

several times in the status display field on LCD panel after the outer door is closed even when the 

humidifying pan is full. 

CAUTION 

Foreign particles on the water surface can adhere to the water level sensor and fittings by capillary action 

because the water level sensor is always in the water. The adhered foreign particles degrade water level 

sensor performance and “RH PAN” may be displayed in reverse video in the status display field on LCD 

panel even though there is sufficient water in the humidifying pan. Be sure to wipe OFF any dirt on the 

water level sensor with alcohol for disinfection whenever you change the humidifying water. When 

cleaning the water level sensor, take care not to apply excessive force to the lead wires. 

22

ROUTINE MAINTENANCE 

To use this unit in a clean condition, clean the chamber and all the inner attachments at least once a 

month. 

1. Remove all the inner attachments by the procedures shown on page 18. 

2. Clean the chamber and all the inner attachments by the procedures shown on page 17. 

3. Install all the inner attachments by the procedures shown on page 20. 

● When there is excessive dirt, contact our sales representative or agent. 

CORRECT OPERATION 

Use the following procedure to start trial operation or actual operation of the incubator. 

1. Install the incubator correctly, referring to “INSTALLATION” on page 13. 

2. Remove the packing materials from the chamber and inner attachments. Clean and disinfect the 

chamber and all the inner attachments, referring to above “ROUTINE MAINTENANCE”. 

3. Add approximately 1.5 L of sterile distilled water to the humidifying pan. (Refer to page 21.) 

4. Connect the power supply cord to the outlet. Turn ON the power switch on the left side of the rear panel 

of the incubator. 

Note: 

The humidity in the incubator chamber is adjusted to the optimum setting. To prevent condensation on the 

surface inside chamber and the inner door, there is a low-temperature area under the humidifying pan in 

the bottom of chamber to recondense evaporated moisture. Condensation may occur around the 

humidifying pan at the bottom of the chamber (on the inside of the humidifying pan cover), but this does 

not indicate a problem. 

23

LCD PANEL 

The following display (called the “Top Display”) will appear when the power switch is turned ON. The 

default temperature is 37.0 o C and the default CO 2 density is 0 %. The date and time are preset at the 

factory. Refer to page 36 to change the date and time. 

1 

0 

1 

0 

2 

1 

1 

1 

2 

1 

3 

1 

4 

3 

1 

5 

1 

6 

1 

7 

1 

8 

1 

9 

2 

0 

2 

1 

2 

2 

2 

3 

2 

4 

2 

5 

2 

6 

2 

7 

2 

8 

2 

9 

3 

0 

5 

3 

1 

3 

2 

3 

3 

3 

4 

3 

5 

3 

6 

3 

7 

3 

8 

3 

9 

4 

0 

2 

6 

7 

1 T e m p 3 7 . 0 o 

C C O 2 5 . 0 % A B 

2 

3 

4 

37.0 5.0 

5 S t a t u s U V R H P A N D o o r : O p e n 

6 O K 

7 

4 

1. Display of set value of temperature 

The set value of chamber temperature is displayed. 

2. Display of current temperature 

The current chamber temperature is displayed. 

3. Display of set value of CO 2 density 

The set value of the chamber CO 2 density is displayed. 

4. Display of current CO 2 density 

The current chamber CO 2 density is displayed. 

5. Display of current CO 2 cylinder 

A and B will be displayed if an optional gas auto changer (MCO-21GC) is installed. The port of the CO 2 

gas pipe that is currently supplying CO 2 will be displayed in reverse video. Nothing will be displayed if the 

MCO-21GC is not installed. 

6. Status display field 

Various status or alarms are displayed. 

・When the UV lamp is lit: “UV” is displayed in reverse video. (If the MCO-19AIC(UVH)/19AIC(UV) or the 

optional MCO-19UVS is installed). 

・When the humidifying water is low: “RH PAN” is displayed in reverse video and blinks. 

・When the door is open: “Door: Open” is displayed in reverse video. 

7. Message display field 

A message is displayed when fault occurs. The message is displayed alternately in normal characters 

and reverse video. Refer to page 46-48 for alarm details. “OK” is displayed during normal operation. 

24

BASIC OPERATIONS ON CONTROL PANEL 

The following operations are possible through the control panel: 

• Setting the temperature・・・The chamber temperature can be set (page 26). 

• Setting the CO 2 density・・・The chamber CO 2 density can be set (page 26). 

• Setting the key lock・・・Changing the temperature and CO 2 density can be disabled (page 27). 

• Setting alarms・・・The temperature alarm, the CO 2 density alarm (page 31), and the high limit alarm 

temperature (page 30) can be set. 

• Setting UV parameters・・・ON/OFF parameters and the ON period of the UV lamp can be set for the 

MCO-19AIC(UVH)/19AIC(UV) or when the incubator is equipped with the optional MCO-19UVS (page 

32). 

• Setting other parameters・・・The initial settings of the date, time, and the log cycle (page 36), and the 

LCD display and baud rate (page 38) can be set. 

• Displaying the operation log and transferring data・・・A graph of past operation data can be 

displayed (page 39) and operation data can be transferred to a PC (page 40). 

• H 2 O 2 decontamination・・・H 2 O 2 decontamination of the chamber and the inner attachments can be 

performed when the optional H 2 O 2 generator (MCO-HP) is installed (page 42). 

• Automatic CO 2 cylinder changeover・・・The cylinder can be switched when the optional gas auto 

changer (MCO-21GC) is installed (page 58). 

• Setting the CO 2 standard gas density・・・The CO 2 standard gas density can be set when the optional 

STD gas auto calibration kit (MCO-SG) is installed. (page 60) 

25


Setting chamber temperature and CO 2 density 

The setting procedure for the chamber temperature and the CO 2 density are given below. (Default 

settings: Chamber temperature: 37 o C, CO 2 density: 0 %) 

The incubator automatically starts operation using these settings after the power is turned ON. 

1. From the “Top Display”, press the menu key (MENU) to access the menu. Select “Set” and press the 

enter key (ENT). 

1 T e m p 3 7 . 0 o C C O 2 5 . 0 % 

2 

3 

4 

5 

6 

37.0 5.0 

S t a t u s 

O K 

M E N U 

S e t 

L o g 

T o o l s 

7 

2. The “Stand-by Setting” display will be displayed. Set the parameters. 

1 

2 

3 

4 

5 

S t a n d - b y S e t t i n g 

M E N U 

T e m p e r a t u r e 3 7 . 0 o C ( 0 . 0 o C - 5 0 . 0 o C ) 

O K 

C O 2 D e n s i t y 5 . 0 % ( 0 . 0 % - 2 0 . 0 % ) 

C a n c e l 

K e y L o c k 0 ( 0 . U n l o c k 1 . L o c k ) 

6 

H i g h L i m i t 5 2 . 0 o C 

The setting ranges of the parameters are as follows: 

●Temperature: 0 o C to 50 o C. To 36.5 o C, enter 365. 

●CO 2 density: 0 % to 20 %. To set 5.0 %, enter 050. 

3. Press the menu key (MENU) to display the menu after setting the parameters. Select “OK” and press 

the enter key (ENT). The settings will be saved. 

Note: 

When starting the incubator for the first time or after not using it for an extended period of time, allow at 

least 4 hours for the chamber temperature, the humidity, and the CO 2 sensor to become stable after 

setting the desired chamber temperature at a 0 % CO 2 density. Then change the setting to the desired 

CO 2 density. 

26

Setting key lock 

1. To set the key lock, change the value of the key lock parameter from 0 to 1 on the “Stand-by Setting” 

display and press the enter key (ENT). The buzzer will sound briefly and the keys will be locked. 

1 

2 

3 

4 

5 


M E N U 


O K 

C O 2 D e n s i t y 5 . 0 % ( 0 . 0 % - 2 0 . 0 % ) 

C a n c e l 


6 

H i g h L i m i t 5 2 . 0 o C 

2. “Key Lock” will be displayed in reverse video at the right side of “Stand-by Setting”, and changing the 

temperature and CO 2 density settings will be disabled. 

Removing key lock 

1. To remove the key lock, change the value of the key lock parameter from 1 to 0 on the “Stand-by 

Setting” display and press the enter key (ENT). 

1 

2 

3 

4 

5 

S t a n d - b y S e t t i n g K e y L o c k 


C O 2 D e n s i t y 5 . 0 % ( 0 . 0 % - 2 0 . 0 % ) 


6 

H i g h L i m i t 5 2 . 0 o C 

2. The cursor will move to the Password field. Input the 4-digit password (default: 0000) and press the 

enter key (ENT). When key lock is release, the buzzer will sound briefly, and “Key Lock” will disappear 

from the “Stand-by Setting” display. 

1 

2 

3 

4 

S t a n d - b y S e t t i n g K e y L o c k 

T e m p e r a t u r e 3 7 . 0 o C ( 0 - 5 0 . 0 o C ) 

C O 2 D e n s i t y 5 . 0 % ( 0 . 0 % - 2 0 . 0 % ) 

K e y L o c k 0 P a s s w o r d 

H i g h L i m i t 5 2 . 0 o C 

27


3. Press the menu key (MENU) to display the menu, select “OK” and press the enter key (ENT). The 

settings will be saved. 

Note: The buzzer will sound for a long time if the wrong password is entered. Enter the correct password. 

The password for releasing the key lock must be shared and administered among all users of the 

incubator. The password is set to 0000 when the incubator is shipped from the factory. The procedure for 

changing the password is given next. 

Setting key lock password 

1. From the “Top Display”, press the menu key (MENU) to display the menu, select “Tools”, and press the 


1 T e m p 3 7 . 0 o C C O 2 5 . 0 % 

2 

3 

4 

5 

6 

37.0 5.0 

S t a t u s 

O K 

M E N U 

S e t 

L o g 

T o o l s 

7 

2. Select “Key Lock PW Setting” from the “Select Tools” display, press the menu key (MENU) to display 

the menu, select “OK”, and press the enter key (ENT). 

S e l e c t T o o l s ( 1 / 2 ) 

T e m p / C O 2 C a l i b r a t i o n 

A l a r m S e t t i n g 

L C D / D A Q S e t t i n g 

U V S e t t i n g 

K e y L o c k P W S e t t i n g 

M E N U 

O K 

S v C 

C a n c e l 

D a t e 

T i m e 

28

3. Input the current user password (4 digits), select “OK”, and press the enter key (ENT). (The default 

user password is 0000 when the incubator is shipped from the factory.) 


C u r r e n t U s e r P a s s w o r d * * * * 

4. Enter the new user password (4 digits), select “OK”, and press the enter key (ENT). 


N e w U s e r P a s s w o r d * * * * 

5. Enter the new user password again, select “OK”, and press the enter key (ENT) to save. After that 

press the menu key (MENU) to display the menu, and select “Cancel“ from the menu. 


N e w U s e r P a s s w o r d **** 

R e E n t e r U s e r P a s s w o r d * * * * 

Note: 

Be careful not to forget the key lock password. If you have forgotten the password and cannot release the 

key lock, contact our sales representative or agent. 

29


Setting high limit alarm temperature 

The high limit temperature alarm is provided with the incubator. The alarm temperature can be changed 

by using the following procedure. 

1. In the “Stand-by Setting” display, turn the high limit regulator on the control panel using a small 

screwdriver to set the desired high limit alarm temperature. The high limit temperature can be set 

between 34 o C and 52 o C. 

1 

2 

3 

4 

5 


M E N U 


O K 

C O 2 D e n s i t y 5 . 0 % ( 0 . 0 % - 2 0 . 0 % ) 

C a n c e l 


6 

H i g h L i m i t 5 2 . 0 o C 

Note: 

・Set the high limit alarm temperature to at least 5 o C higher than the chamber set temperature. 

・Set the high limit alarm temperature to at least 50 o C when H 2 O 2 decontamination is performed. 

2. After setting the parameter, press the menu key (MENU) to display the menu, select “OK”, and press 

the enter key (ENT). The alarm temperature will be saved. 

Refer to the tables of “ALARMS, SAFETY, AND SELF-DIAGNOSIS” on page 46 for details. 

Note: 

・The high limit alarm temperature will be changed at any time by turning the high limit regulator even if 

the “Stand-by Setting” display is not displayed. 

・Set the high limit alarm temperature after the chamber reaches the set temperature for operation. 

30

ALARM PARAMETERS 

1. Press the menu key (MENU) from the “Top Display” to display the menu, select “Tools”, and press the 


1 T e m p 3 7 . 0 o C C O 2 5 . 0 % 

2 

3 

4 

5 

6 

37.0 5.0 

S t a t u s 

O K 

M E N U 

S e t 

L o g 

T o o l s 

7 

2. Select “Alarm Setting” from the “Select Tools” display, press the menu key (MENU) to display the menu, 

select “OK”, and press the enter key (ENT). 

S e l e c t T o o l s ( 1 / 2 ) 






M E N U 

O K 

S v c 

C a n c e l 

D a t e 

T i m e 

3. The “Alarm Setting” display will appear. The temperature alarm, the CO 2 alarm, the alarm delay, the 

ring back, and the door delay can be set on this display. The alarm buzzer can be silenced by pressing 

the buzzer key (BUZZER). The alarm buzzer will sound again after the specified ring back time if the 

condition that caused the alarm continues. The ring back time can be set. 

1 

3 

4 

5 

6 


M E N U 

T e m p A l a r m ± 1 . 0 o C ( ± 1 . 0 ° C - ± 5 . 0 ° C ) 

C O 2 A l a r m ± 0 . 5 % ( ± 0 . 5 % - ± 

O K 

5 . 0 % ) 

C a n c e l 

A l a r m D e l a y 1 5 m i n ( 0 - 1 5 m i n ) 

R i n g B a c k 3 0 m i n ( 0 . O F F 1 - 9 9 m i n ) 

D o o r D e l a y 2 m i n ( 1 - 3 0 m i n ) 

・Temp Alarm: ±1.0 o C to ±5.0 o C (Default: ±1.0 o C) 

・CO 2 Alarm: ±0.5 % to ±5.0 % (Default: ±1.0 %) 

・Alarm Delay: 0 minute to 15 minutes (Default: 15 minutes) 

・Ring Back: 1 minute to 99 minutes, or OFF (Default: 30 minutes) 

・Door (alarm) Delay: 1 minute to 30 minutes (Default: 2 minutes) 

31


The UV lamp is used with the MCO-19AIC(UVH)/19AIC(UV) or the MCO-19AIC with an MCO-19UVS UV 

system set installed. Use the following procedures to make the settings. 

Using UV lamp 

The UV lamp is located inside the duct to sterilize the water in the humidifying pan and the air circulating 

in the chamber. Observe the following points to use the UV lamp correctly. 

• When all the inner attachments are installed correctly, only the inside of the duct and the inside of the 

humidifying pan cover are exposed to UV light. 

• Correctly install all of the inner attachments when starting a cell culture, and never turn ON the UV lamp 

when the humidifying pan cover is removed. 

• Always install the humidifying pan cover even when using the incubator without turning ON the UV 

lamp. Leaving the cover uninstalled will affect the chamber temperature distribution and the humidity 

recovery performance. 

• The UV lamp stays lit for a preset period of time after the outer door is closed. The default setting is for 

five minutes. 

• If the outer door is not opened for at least 12 consecutive hours, the UV lamp will light for the preset 

time period every 12 hours. 

• The recommended replacement time for the UV lamp (i.e., when the UV output ratio drops to 60 % to 

70 % of its initial value) is when the accumulated ON time reaches 1,000 hours. When the accumulated 

ON time reaches approximately 1,000 hours, the “UV” will blink in the status display field on the LCD 

panel (when the UV lamp is not lit) and “Warning :UV Life” is displayed in the message display field on the 

LCD panel. It is recommended that the UV lamp be quickly replaced at this point. Contact our sales 

representative or agent for information on replacing the UV lamp. 

• If the UV lamp burns out, “Err18:UV Lamp Abnormal” will be displayed in the message display field. If 

this occurs, replace the UV lamp. When replacing the UV lamp, replace the glow starter (type FG-7P) at 

the same time. Contact our sales representative or agent for information on replacing the UV lamp. 

• If the UV lamp burns out (“Err18:UV Lamp Abnormal” will be displayed in the message display field), it 

will not be possible to perform H 2 O 2 decontamination. Replace the UV lamp and the glow starter (type 

FG-7P). 

32

Precautions when using UV lamp 

WARNING 

Do not look directly at UV light. UV light is harmful to the eyes. 

• Always use the humidifying pan and humidifying pan cover. 

The humidifying pan and humidifying pan cover prevent UV light from escaping. Always use them even 

when not humidifying. To check whether the UV lamp is lit, open the outer door and then press the door 

switch with the inner door still closed. Visible blue light can be confirmed from the front of the humidifying 

pan cover. UV light is harmful to the eyes, so do not light the UV lamp when the inner door or the 

humidifying pan cover is open. 

• Be careful when handling the UV lamp. 

There is a UV lamp inside of the duct. Be careful not to damage it when installing or removing the inner 

attachments, the humidifying pan, or the H 2 O 2 generator. 

33


Setting UV lamp ON period 

Use the following procedure to change the setting of the UV lamp ON period. 



1 T e m p 3 7 . 0 o C C O 2 5 . 0 % 

2 

3 

4 

5 

6 

37.0 5.0 

S t a t u s 

O K 

M E N U 

S e t 

L o g 

T o o l s 

7 

2. Select “UV Setting” from the “Select Tools” display, press the menu key (MENU) to display the menu, 


S e l e c t T o o l s ( 1 / 2 ) 






M E N U 

O K 

S v c 

C a n c e l 

D a t e 

T i m e 

3. The “UV Setting” display will appear. Set the UV timer for the UV lamp ON period. 

1 

3 

4 

5 


U V T i m e r 5 mi n ( 0 - 3 0 m i n ) 

U V L i f e 0 % 

U V T i m e r E x t . + 0 % 

U V 2 4 h M o d e 0 ( 0 . O F F 1 . O N ) 

6 

4. Press the menu key (MENU) to display the menu, select “OK”, and press the enter key (ENT). The 

parameter will be saved. 

34

The UV timer can be set from 0 minute to 30 minutes. The default setting is for 5 minutes. When the UV 

timer is set to zero, the UV lamp will not light. 

If the outer door is opened while the UV lamp is lit, the lamp will turn OFF. Then, when the outer door is 

closed, the lamp will light for the preset period of time. 

If the UV lamp ON time is set for longer than 10 minutes or if only the outer door is repeated opened 

and closed, condensation may occur in the chamber and may affect temperature distribution. It will also 

shorten the service life of the UV lamp. 

When replacing the UV lamp, contact our sales representative or agent. 

Note: To compensate for the drop in UV ray output along with increased accumulated ON time of the UV 

lamp, the incubator automatically extends the UV lamp ON time according to the accumulated ON time. 

The automatically extended time is displayed as a percentage under “UV Timer Ext.” in the “UV Setting” 

display. 

Lighting UV lamp for 24 hours 

If the chamber has been contaminated by dirt or by spilling the medium, use the following procedure to 

decontaminate the chamber by lighting the UV lamp for 24 hours. 

1. Remove all attachments from the chamber, including the trays, the tray supports, the side supports, the 

fan cover, the duct, the fan, the humidifying pan, and the humidifying pan cover. Clean all the 

attachments in an autoclave or with alcohol for disinfection. 

2. Clean and wipe off inside the chamber with alcohol for disinfection. 

3. Set the CO 2 density to 0 % and set the UV 24-hour mode to 1 on the “UV Setting” display. 

1 

U V 

S e t t i n g 

3 

4 

5 

U V T i m e r 5 m i n ( 0 - 3 0 m i n ) 

U V L i f e 0 % 

U V T i m e r E x t . + 0 % 

U V 2 4 h M o d e 1 ( 0 . O F F 1 . O N ) 

6 

4. Press the menu key (MENU) to display the menu, select “OK”, and press the enter key (ENT). The 

parameter will be saved. 

・This procedure must be preformed only with the outer door closed and the UV lamp turned OFF. 

・The UV lamp will light continuously for 24 hours after this parameter is set. The setting is canceled if the 

outer door is opened. Perform the above procedure to set the UV 24-hour mode again if you open the 

outer door. 

・Install all attachments after completion of the UV 24-hour mode. 

Note: Set the high limit alarm temperature to at least 10 o C higher than the chamber set temperature 

when using the UV 24-hour mode. The UV 24-hour mode may cause the automatic set temperature 

alarm because the temperature of the chamber will increase. 

35


Setting date, time, and log interval 

1. Press the menu key (MENU) from the “Top Display” to display the menu, select “Tools” and press the 


1 T e m p 3 7 . 0 o C C O 2 5 . 0 % 

2 

3 

4 

5 

6 

37.0 5.0 

S t a t u s 

O K 

M E N U 

S e t 

L o g 

T o o l s 

7 

2. Select “Date Time” from the “Select Tools” display, press the menu key (MENU) to display the menu, 


S e l e c t T o o l s ( 1 / 2 ) 






M E N U 

O K 

S v c 

C a n c e l 

D a t e 

T i m e 

36

3. The “Date Time” display will appear. Set the date, time and log interval. 

1 

D a t e 

T i m e 

M E N U 

3 

4 

5 

6 

D a t e 0 9 / 0 3 / 0 1 ( Y Y / M M / D D ) 

T i m e 1 0 : 0 5 : 0 0 ( h h : m m : s s ) 

L o g I n t e r v a l 6 m i n ( 2 - 3 0 m i n ) 

O K 

C a n c e l 

・Entering the date 

Example for March 1, 2009: Enter 090301 in the “Date Field”. 

・Entering the time 

Example for 10:05:00: Enter 100500 in the “Time Field”. 

・Entering the log interval 

Example for 6 minutes: Enter 6 in “Log Interval Field”. 

Note: 

■ The default is 6 minutes. 

■ The log interval can be set to between 2 minutes and 30 minutes. 

■ Relation between the log interval and the period that can be saved 

1: Log interval of 2 minutes -- About 5 days 



For the data beyond the above period, the older data is deleted to save the newer data. 

37


Initial settings (LCD/DAQ parameters) 

1. Select “LCD/DAQ Setting” from the “Select Tools” display, press the menu key (MENU) to display the 

menu, select “OK”, and press the enter key (ENT). 

S e l e c t T o o l s ( 1 / 2 ) 






M E N U 

O K 

S v c 

C a n c e l 

D a t e 

T i m e 

2. The “LCD/DAQ Setting” display will appear. Set the initial setting for each parameter as necessary. 

1 

3 

4 

5 


M E N U 

L C D B a c k C o l o r 1 ( 1 . B l u e 2 . W h i t e ) 

D A Q S p e e d 0 ( 0 . 2 4 2 . 9 6 

O 

3 

K 

. 3 5 0 ) 

D A Q I D 0 ( 0 . O F F 1 - 2 C 5 a 5 n ) c e l 

D A Q M o d e 0 ( 0 . L o c a l 1 . R e m o t e ) 

R e m o t e A l a r m 0 ( 0 . O F F 1 . A c t i v e ) 

6 

・LCD Back Color: Setting of the background color (1: Blue, 2: White) 

Note: 

DAQ is an external monitoring system of the chamber status. It is necessary to set the DAQ speed, DAQ 

ID, and DAQ mode to use the optional communications software. Communications software is an 

optional accessory. Contact our sales representative or agent for details. 

38

DISPLAYING THE LOG 

A log of the past chamber temperatures and CO 2 densities can be displayed on a graph. 

1. Press the menu key (MENU) from the “Top Display” to display the menu, select “Log” and press the 


1 T e m p 3 7 . 0 o C C O 2 5 . 0 % 

2 

3 

4 

5 

6 

37.0 5.0 

S t a t u s 

O K 

M E N U 

S e t 

L o g 

T o o l s 

7 

2. The log will be displayed with dots. Press the “Up Cursor key” and “Down Cursor key” to switch 

between the temperature and CO 2 density displays. Press the “Left Cursor key” and “Right Cursor key” to 

scroll the displayed data (Left Cursor key: older data, Right Cursor key: newer data). 

1 5 0 ° C 2 0 0 9 / 0 3 / 0 1 T e m p 

3 

Temperature 

4 

5 

6 0 1 2 2 4 

Switched with Up and Down Cursor keys 

CO 2 density 

1 

2 0 % 2 0 0 9 / 0 3 / 0 1 C O 2 

3 

4 

5 

6 0 1 2 2 4 

39

DISPLAYING THE LOG 

Transferring data 

Use the following procedure to transfer the log data to a PC. 

1. To transfer the log data for one day, press the menu key (MENU) to display the menu, select “PC 1D”, 

and press the enter key (ENT). To transfer all of the log data, select “PC All” and press the enter key 

(ENT). 

1 

3 

4 

5 

5 0 ° C 2 0 0 9 / 0 3 / 0 1 T e m p 

M E N U 

P C 1 D 

P C A l l 

C l e a r 

C a n c e l 

6 0 1 2 2 4 

2. A “Progress” display will appear. On a HyperTerminal on PC, specify a transfer, text capture, and the 

name of the file to save. Use TXT or CSY as the file name extension. Press the menu key (MENU) to 

display the menu, select “Start”, and press the enter key (ENT). 

The transfer will be started. “Finished” will be displayed when the transfer has been completed. Select 

“Cancel“ from the menu after the transfer has been completed and press the enter key (ENT). 

1 

3 

4 

5 

P r o g r e s s 

S e n d l o g d a t a t o P C . 

L o g D a t a 2 0 0 9 / 0 3 / 0 1 

M E N U 

S t a r t 

C a n c e l 

6 

F i n i s h e d . 

Setting in PC side for transmission of log data (For Windows 2000 and Windows XP) 

1. From the Windows Start Button, select Program - Accessories - Communications - 

HyperTerminal to start the HyperTerminal. (If the HyperTerminal is not available from the Start Menu, 

execute the following file: C:¥Program Files¥Windows NT¥hypertrm.exe.) 

2. In the HyperTerminal Window, set a new connection, the name (for example, Matsushita), connection 

settings, method of connection, COM1, properties of COM1, and port. 

Baud rate: 9,600, Data bits: 8, Parity: None, Stop bits: 1, Flow control: Xon/Xoff. 

(The communications condition of the incubator will be set automatically to the above settings when the 

“Progress” display appears.) 

Note: An optional interface board MTR-480 (RS-232C/RS-485) is required to transfer data. 

40

H 2 O 2 DECONTAMINATION 

When the chamber has been contaminated, or when cleaning the chamber prior to starting a culture, 

H 2 O 2 decontamination can be performed using the H 2 O 2 generator. 

• The following products must be purchased separately in order to perform H 2 O 2 decontamination. Before 

performing H 2 O 2 decontamination, make sure that these are correctly installed. 

UV system set (MCO-19UVS) (Not required for the MCO-19AIC(UVH)/19AIC(UV).) 

H 2 O 2 decon set (MCO-HL) 

H 2 O 2 generator (MCO-HP) 

• The H 2 O 2 decontamination function decontaminates the chamber and the inner attachments. Do not 

place any objects other than the specified attachments in the chamber during decontamination. 

• Before performing H 2 O 2 decontamination, clean the chamber and the inner attachments with alcohol for 

disinfection. 

• Before performing H 2 O 2 decontamination, make sure that the duct, the fan and the H 2 O 2 generator are 

securely attached. (Fig. 1 - 3 on page 52) 

• The chamber temperature during H 2 O 2 decontamination reaches 45 C. Before performing H 2 O 2 

decontamination, set the high limit temperature alarm to 50 C or higher. 

• For the H 2 O 2 reagent, use one bottle of the H 2 O 2 reagent specified by our company (MCO-H2O2). 

• After decontamination, dilute the residual H 2 O 2 reagent in the H 2 O 2 generator with a large quantity of 

water and dispose of it, and rinse with distilled water. (Do not wash either the inside or outside of the H 2 O 2 

generator with alcohol.) 

WARNING 

Use the reagent specified by our company for H 2 O 2 decontamination. Using a different H 2 O 2 solution 

may cause explosion or damage to the incubator, or insufficient decontamination. 

Do not use chemicals other than the H 2 O 2 reagent, such as alcohol. Doing so may result in damage 

to the H 2 O 2 generator. 

WARNING 

When performing H 2 O 2 decontamination, make sure that the outer and inner doors are securely 

closed. During H 2 O 2 decontamination, plug the access hole with the silicon caps that are provided. Failure 

to do so may be harmful to health due to leakage of H 2 O 2 gas. 

CAUTION 

H 2 O 2 decontamination can be performed only for the chamber and inner attachments with standard 

specifications, and not for any other objects. 

CAUTION 

Perform H 2 O 2 decontamination with the inner attachments arranged as specified by our company. 

Arranging them in a different way may result in insufficient decontamination. 

CAUTION 

Wear rubber gloves when handling the H 2 O 2 reagent. Direct contact with the H 2 O 2 reagent may result 

in inflammation of the skin. 

CAUTION 

After H 2 O 2 decontamination has been completed, residual H 2 O 2 solution will remain on the bottom of the 

chamber, the H 2 O 2 generator, and the bottom of the duct. Wearing protective glasses and rubber 

gloves, wipe it off with a non-woven cloth. Failure to do so may result in a deficient culture. 

41


Use the following procedure to perform H 2 O 2 decontamination using the H 2 O 2 generator (MCO-HP). 

1. Take out all the trays, the tray supports, the side supports, the humidifying pan cover, the humidifying 

pan, the fan cover, and the duct from the chamber. Dispose of the water in the humidifying pan, wipe the 

inside walls of the chamber with a gauze containing water or alcohol for disinfection. 

2. Attach the duct and side supports, and set the tray supports in the 2nd, 5th, 7th, 9th holes from the top 

of the side supports. Then set the trays. 

3. Pour one bottle of the H 2 O 2 reagent (MCO-H2O2) into the H 2 O 2 generator. Set the two pins on the 

H 2 O 2 generator in the 2 holes on the lower left side of the duct (Fig. 1). 

4. Connect the H 2 O 2 generator and connector on the bottom right of the far side of the chamber with the 

enclosed cable. Be sure to keep the connector cap (Fig. 2). 

5. Set the humidifying pan cover, the humidifying pan and the fan cover as shown in Fig. 3, and close the 

inner door and the outer door. 

Note: The trays included as accessory are designed to be appropriate for decontamination. If half tray 

(MCO-25ST, optional) or trays for previous models are used, decontamination may not be sufficiently 

effective. 

Duct end 

Connector 

H 2 O 2 generator 

Fig. 1 

Fig. 2 

Front 

Humidifying 

pan cover 

Fan cover 


H 2 O 2 

Generator 

Fig. 3 

42

6. Press the H 2 O 2 key (H2O2) for 5 seconds. The system check will start. 

1 

H 2 O 2 D e c o n t a m i n a t i o n 

3 

4 

H 2 O 2 D e c o n S y s t e m C h e c k ・・・・・ 

5 

6 

7. If the system is normal, the following display will appear. (If the system is not normal, refer to Table 2 

on page 47.) Select “OK” and press the enter key (ENT) to start H 2 O 2 decontamination. Decontamination 

will be performed automatically to step 10. The buzzer will sound when decontamination has been 

completed (It takes about 1 hour 40 minutes after warming-up). 

Note: The outer door will be locked with the electric lock for safety until the decontamination has been 

completed. 

1 

3 

4 

5 

H 2 O 2 D e c o n t a m i n a t i o n 

R e a d y t o S t a r t 

M E N U 

O K 

C a n c e l 

6 

8. During the H 2 O 2 mist generation, “H2O2 Decon” will blink at the top left corner of the screen. 

Note: H 2 O 2 mist generates from the H 2 O 2 generator and then quickly changes to a gas. 

1 H 2 O 2 D e c o n U V R e s o l v e S t e p : 4 

2 

3 

4 

5 

6 

7 

7min 

90min 

T e m p : 4 5 . 0 ℃ 

D o o r : L o c k e d 

43


9. After completion of H 2 O 2 mist generation, “UV Resolve” will blink at the top of the screen, and H 2 O 2 gas 

resolution by UV will start. 

1 H 2 O 2 D e c o n U V R e s o l v e S t e p : 7 

2 

3 

4 

5 

6 

7 

0min 

89min 

T e m p : 4 5 . 0 ℃ 

D o o r : L o c k e d 

10. After H 2 O 2 gas resolution, H 2 O 2 decontamination has been completed. The following display will 

appear. Open the outer door and the inner door, then disconnect the cable of the H 2 O 2 generator from the 

connector in the chamber. Then take out the H 2 O 2 generator. 

Note: Always put on protection glasses and rubber gloves when performing decontamination. 

1 

2 

3 

H 2 O 2 D e c o n t a m i n a t i o n S t e p : 8 

D e c o n F i n i s h e d . 

4 

5 

6 

T e m p : 4 5 . 0 ℃ 

P l e a s e R e m o v e H 2 O 2 U n i t D o o r : U n l o c k 

7 

11. The following display will appear. Press the menu key (MENU) to display the menu, select “OK”, and 

press the enter key (ENT) to return to the “Top Display”. 

1 

2 

3 

H 2 O 2 D e c o n t a m i n a t i o n S t e p : 8 

D e c o n F i n i s h e d . 

4 

5 

6 

T e m p : 4 5 . 0 ℃ 

P U S H M E N U O K D o o r : U n l o c k 

7 

44

12. Dilute the remaining H 2 O 2 reagent in the H 2 O 2 generator with a large 

volume of water and dispose of it. Rinse and wash the H 2 O 2 generator with 

distilled water. Then keep the H 2 O 2 generator in a clean environment outside 

of the chamber. 

Note: After H 2 O 2 decontamination, cover the connector on the chamber side 

with the connector cap deeply. (Fig. 1) 

13. After H 2 O 2 decontamination, surplus H 2 O 2 liquid will remain at the bottom 

of the chamber and in the bottom part of the H 2 O 2 generator duct. This 

solution contains H 2 O 2 at a low density (about 1 % or less), so put on 

protective glasses and rubber gloves and wipe it up with a non-woven cloth. 

Fig. 1 

14. Ventilate the chamber sufficiently and place all the attachments back into the chamber. 

Note: Be sure to attach the connector cap. 

CAUTION 

The electric lock will remained locked if power fails during H 2 O 2 decontamination. After the power comes 

back ON, the H 2 O 2 gas resolution process will start execution and finish automatically. Execute the 

decontamination again because the decontamination will be incomplete. 

WARNING 

Never open the outer door by unlocking it with the unlock key during H 2 O 2 decontamination or H 2 O 2 gas 

resolution with the UV lamp. H 2 O 2 gas leakage is potentially harmful to health. 

Precautions when handling H 2 O 2 reagent 

Observe the following precautions when handling the H 2 O 2 reagent (MCO-H2O2). 

• Handling precautions 

Wear protective equipment, such as protective glasses and rubber gloves. 

Do not use fire in the area where the reagent is being handled. 

Do not leave any reagent in the container after it has been used or while it is being used. 

Do not place inflammable or combustible materials near the area where the reagent is handled. 

• Precautions for storage 

Always close the container cover securely to prevent impurities from becoming mixed in the reagent. 

Check the container to make sure that there is no damage, corrosion, or cracking. 

Store the container with the inlet facing upwards, and make sure that the container will not tip or be 

knocked over. 

If possible, store the reagent in a location where water is available and where the floor can be easily 

washed. 

• Precautions for disposal 

Dispose according to the rule in your country. 

45

ALARMS, SAFETY, AND SELF-DIAGNOSIS 

The incubator supports the following alarms, safety functions, and self-diagnostic functions. 

Table 1: Alarms, safety, and self-diagnosis for culture operations 

Alarm or safety 

Conditions Display Buzzer Safety operation 

function 

High limit 

temperature 

alarm 

Automatic set 

temperature 

alarm 

Automatic set 

CO 2 density 

alarm 

Auto-return 

Key lock 

Door alarm 

CO 2 gas 

cylinder empty 

CO 2 line 

changeover 

Chamber 

temperature 

sensor error 

Sensor box 

temperature 

sensor error 

Ambient 

temperature 

sensor error 

CO 2 sensor 

error 

Main heater 

error 

Bottom heater 

error 

Door heater 

error 

Sensor box 

heater error 

Heater SSR 

burnout 

Low humidifying 

water 

The chamber temperature exceeds the 

high limit alarm temperature set value. 

“Err13: Main Heater Abnormal or 

Err16: CO 2 S Box Heater Abnormal” is 

displayed in the message display field. 

Overheat indicator lights. 

“Warning :High Temp” or 

The chamber temperature is out of the 

“Warning :Low Temp” 

automatic set temperature alarm setting 

range (±1.0 o C to ±5.0 o is displayed in the message display 

C). 

field. 

The chamber CO 2 density is out of the “Warning :High CO 2 Density” or 

automatic set CO 2 density alarm setting “Warning :Low CO 2 Density” is 

range (±0.5 % to ±5.0 %). 


There are no key operations for approx. 

90 s in a setting mode. 

The key lock is ON. 

The outer door is open. 

The CO 2 density does not increase 

when the CO 2 valve is opened. 

Continuous tone 

Heater OFF 

Remote alarm 

operates 

Intermittent tone 

Remote alarm 

after set alarm delay 

operates after delay 

time (0 min to 15 

of 0 min to 15 min. 

min) has elapsed 





(Return to the “Top Display”.) ----- 

“Key Lock” is displayed on the standby 

operation setting screen. 

“Door:Open” is displayed 

in the message display field. 

“Err01: CO 2 Gas Empty” is displayed 


“Err01: CO 2 Gas Empty” is displayed 

The CO 2 gas supply is switched 


automatically to the reserve cylinder 

The text blinks for the empty CO 

(only when the MCO-21GC is installed). 

2 gas 

supply line. 

The chamber temperature sensor is 

disconnected. 

The chamber temperature sensor is 

short-circuited. 

The sensor box temperature sensor is 

disconnected. 

The sensor box temperature sensor is 


The ambient temperature sensor is 

disconnected. 

The ambient temperature sensor is 


The Vref or Vgas output voltage for the 

CO 2 sensor is abnormal. 

Main heater burnout occurs or the main 

heater SSR is short-circuited when the 

high limit temperature alarm operates. 

Bottom heater burnout occurs or the 

bottom heater SSR is short-circuited. 

Door heater burnout occurs or the door 

heater SSR is short-circuited. 

Sensor box heater burnout occurs or 

the sensor box heater SSR is 


Main, bottom, door, or sensor box 

heater SSR burnout occurs. 

The water in the humidifying pan drops 

to approx. 600 ml. 

“Err05: Temp Sensor Open” 

is displayed in the message display 

field. 

“Err06: Temp Sensor Short” 


field. 

“Err07: CO 2 S Box Temp Sensor Open” 


field. 

“Err08: CO 2 S Box Temp Sensor Short” 


field. 

“Err09: At Sensor Open” is displayed in 

the message display field. 

“Err10: At Sensor Short” is displayed in 


“Err11: CO 2 Sensor Vref Abnormal” or 

“Err12: CO 2 Sensor Gas Abnormal” is 


“Err13: Main Heater Abnormal” is 


“Err14: Humidity Heater Abnormal” 


field. 

“Err15: Door Heater Abnormal” is 


“Err16: CO 2 S Box Heater Abnormal” is 


“Err17: Heater SSR Open” is displayed 


“RH PAN” blinks in reverse video in the 

status display field. 

----- 






〃 

〃 

〃 

〃 

〃 

〃 

〃 

〃 

〃 

〃 

〃 

〃 

〃 

Remote alarm 

operates after delay 

of 0 min to 15 min. 

The setting mode is 

canceled. 

Setting changes are 

prohibited. 

The CO 2 valve is 

closed. 

The heater turns 

OFF after 1 min. 

Remote alarm 

operates. 

The CO 2 gas supply 

is switched. 

Remote alarm 

operates. 


OFF. 

Remote alarm 

operates. 


OFF. 

Remote alarm 

operates. 

CO 2 valve is closed. 

Remote alarm 

operates. 


Remote alarm 

operates. 

Remote alarm 

operates. 

Remote alarm 

operates. 


Remote alarm 

operates. 

Remote alarm 

operates. 

Remote alarm 

operates. 

Remote alarm 

operates. 

Remote alarm 

operates. 

Remote alarm 

operates. 

----- ----- 

46

Alarm or safety 

function 

UV lamp failure 

New UV lamp 

recommended 

Conditions Display Buzzer Safety operation 

The UV lamp burns out (only for the 

MCO-19AIC(UVH)/19AIC(UV) or when 

the optional MCO-19UVS is installed). 

The accumulated ON time reaches 

approx. 1,000 h (only for the 

MCO-19AIC(UVH)/19AIC(UV) or when 

the optional MCO-19UVS is installed). 

“Err18: UV Lamp Abnormal” is 


“UV” is displayed in the status display 

field. (When the UV lamp is ON) 

“UV” blinks in the status display field. 

(When the UV lamp is OFF) 

“Warning :UV Life” is displayed in the 

message display field. 


Remote alarm 

operates. 

----- ----- 

The buzzer can be stopped by pressing the buzzer key (BUZZER), but the remote alarms will not stop. 

In addition, the buzzer that sounds because of the high limit temperature alarm cannot be stopped by 

pressing the buzzer key (BUZZER). 

Err01 recovery is achieved automatically by correctly connecting the new CO 2 gas cylinder and pressing 

the buzzer key (BUZZER) to stop the buzzer. In addition, when the MCO-21GC is installed and the gas 

supply is switched to the reserve gas cylinder, press the buzzer key (BUZZER) and replace the gas 

cylinder. 

If an error from Err05 to Err18 occurs (except for the high limit temperature alarm in Err13 to Err16), 

then contact our sales representative or agent. 

Table 2: Alarms and Safety functions for H 2 O 2 decontamination 

Alarm and safety 

Condition Display Buzzer Safety operation 

function 

System check error 

at start of H 2 O 2 

decontamination 

Failure during 

warming-up 

Failure during H 2 O 2 

decontamination 

Failure during H 2 O 2 

gas resolve by UV. 

The H 2 O 2 generator is not 

connected. 

There is no H 2 O 2 solution or 

the H 2 O 2 level sensor has 

failed (or is disconnected). 

The door is not closed. 

Power was interrupted. 


The volume of H 2 O 2 generated 

is abnormal. 

Power was interrupted during 

H 2 O 2 decontamination. 


The UV lamp failed during 

H 2 O 2 gas resolve by UV. 

Power was interrupted during 

H 2 O 2 gas resolve by UV. 


“H2O2 Decon System Error” is displayed 

in the screen center. 

“Err31:H2O2 Unit No Connect” is 




“Err32: H2O2 Level is Low” is displayed 




“Err33: Door is Open” is displayed in the 


After power is restored, return to the 

“Top Display”. 

“Err38:Door Lock Failed” is displayed in 


After completion, “Decon Failed” is 

displayed in the screen center. 

“Err34:H2O2 Volume” is displayed in the 


After power is restored, “Err35: Power 

Failed” is displayed in the message 

display field. 







“Err36: UV Lamp Failure” is displayed in 


After power is restored, “Err37: Power 

Failed” is displayed in the message 

display field. 





47 

----- 

----- 

----- 

Decontamination mode is 

cancelled. 


cancelled. 


cancelled. 

----- ----- 

Continuous 

tone 

(when the 

outer door 

is open.) 

Intermittent 

tone with 

15min delay 

Intermittent 

tone 

Continuous 

tone 

(when the 

outer door 

is open.) 

Intermittent 

tone 

----- 

Continuous 

tone 

(when the 

outer door 

is open.) 


cancelled. 

Remote alarm operates. 

(when the outer door is open.) 

Moves to UV resolve. 

Remote alarm operates with 15 

min. delay. 

Electrically locked during power 

interruption. After power is 

restored, moves to UV resolve. 


H 2 O 2 mist generation is 

cancelled. 

Resolve time is extended. 


(when the outer door is open.) 



Electrically locked during power 

interruption. After power is 

restored, UV resolve is 

repeated. 



(when the outer door is open.)

ALARMS, SAFETY, AND SELF-DIAGNOSIS 

Table 3: Alarm and Safety functions for automatic standard gas calibration 

Alarm and safety Condition Display Buzzer Safety operation 

function 

System check error The gas pressure of CO 2 gas “Std Gas Calib Error” is displayed in the 

The automatic CO 

at start/operating of line for the standard gas screen center. 

Intermittent 

2 density 

calibration is cancelled. 

automatic CO 2 calibration is less than the “Err41: CO2 Std Gas Empty” is tone 


density calibration specified pressure. 


Note: 

For models with electric locking, an unlock key is provided in order to unlock the outer door during a 

power interruption or in case the electric lock fails. Always store this key in a safe place. It is 

recommended that you make a note of the key symbol and number in case the key is lost. 

WARNING 

Do not use the unlock key to unlock the outer door during H 2 O 2 decontamination or during H 2 O 2 

gas resolve by UV. Doing so may cause harm to health from H 2 O 2 gas leakage. 

48

CALIBRATION 

Temperature/CO 2 calibration 



1 T e m p 3 7 . 0 o C C O 2 5 . 0 % 

2 

3 

4 

5 

6 

37.0 5.0 

S t a t u s 

O K 

M E N U 

S e t 

L o g 

T o o l s 

7 

2. Select “Temp/CO2 Calibration” from the “Select Tools (1/2)” display, press the menu key (MENU) to 

display the menu, select “OK”, and press the enter key (ENT). 

S e l e c t T o o l s ( 1 / 2 ) 


M E N U 

A l a r m 

S e t t i n g 

O K 




S v c 

C a n c e l 

D a t e 

T i m e 

3. The “Temp/CO2 Calibration” display will appear. 

Example 

Enter the value shown below if the displayed chamber temperature is 37.0 o C but the actual temperature 

is 36.5 o C. 

●Temperature span: Enter 365. 

0 

1 

0 

2 

0 

3 

0 

4 

0 

5 

0 

6 

0 

7 

0 

8 

0 

9 

1 

0 

1 

1 

1 T e m p / C O 2 C a l i b r a t i o n 

1 

2 

1 

3 

1 

4 

1 

5 

1 

6 

1 

7 

1 

8 

1 

9 

2 

0 

3 P V V o l t 

T e m p S p a n 3 6 . 5 o C 3 7 . 0 o C 0 . 0 ° C 

4 C O 2 Z e r o 0 . 1 % 0 . 0 % 4 . 0 2 V 5 0 0 

5 C O 2 S p a n 0 . 0 % 5 . 0 % 4 . 0 1 V 5 0 0 

C O 2 R e f 4 . 0 2 V 

6 C O 2 G a s 4 . 0 1 V 

2 

1 

2 

2 

2 

3 

2 

4 

2 

5 

2 

6 

2 

7 

2 

8 

2 

9 

3 

0 

3 

1 

3 

2 

3 

3 

3 

4 

3 

5 

3 

6 

3 

7 

3 

8 

3 

9 

4 

0 

49

CALIBRATION 

Example 

Enter the value shown below if the displayed chamber CO 2 density is 5.0 % but the actual CO 2 density is 

4.5 %. 

●CO 2 density span: Enter 045. 

0 

1 

0 

2 

0 

3 

0 

4 

0 

5 

0 

6 

0 

7 

0 

8 

0 

9 

1 

0 

1 

1 

1 T e m p / C O 2 C a l i b r a t i o n 

1 

2 

1 

3 

1 

4 

1 

5 

1 

6 

1 

7 

1 

8 

1 

9 

2 

0 

3 P V V o l t 

T e m p S p a n 0 . 0 o C 3 7 . 0 o C 0 . 0 ° C 

4 C O 2 Z e r o 0 . 0 % 4 . 0 2 V 5 0 0 

5 C O 2 S p a n 4 . 5 % 5 . 0 % 4 . 0 1 V 5 0 0 

C O 2 R e f 4 . 0 2 V 

6 C O 2 G a s 4 . 0 1 V 

2 

1 

2 

2 

2 

3 

2 

4 

2 

5 

2 

6 

2 

7 

2 

8 

2 

9 

3 

0 

3 

1 

3 

2 

3 

3 

3 

4 

3 

5 

3 

6 

3 

7 

3 

8 

3 

9 

4 

0 

4. Press the menu key (MENU) to display the menu, select “OK”, and press the enter key (ENT). 

5. After the “Select Tools (1/2)” display is displayed, press menu key (MENU) to display the menu, select 

“Cancel”, and press the enter key (ENT) to return to the “Top Display”. 

Note: 

It is important to accurately measure the temperature inside the incubator when calibrating the 

temperature. Particularly, the temperature gauge used must have an accuracy of JIS 0.5 Class or better. 

The temperature must be measured at several points. 

The temperature setting must not change by more than ±1.0 o C during calibration. If these values are 

exceeded, an error buzzer will sound, the input data will be ignored, and the incubator will return to the 

display mode. If it is necessary to change the temperature by more than 1.0 o C, perform calibration in 

several stages over a period of time. 

50

TROUBLESHOOTING 

If the incubator does not seem to be working properly, check the following items before calling for service. 

Symptom 

The incubator does not 

operate at all. 

The key is not working. 

An alarm has occurred. 

The chamber temperature 

does not match the set value. 

The CO 2 gas density does 

not match the set value. 

The chamber temperature 

does not rise. 

A large quantity of CO 2 gas is 

being consumed. 

Normal cultures are not 

possible, and the CO 2 gas 

density is suspect. 

Items to check and countermeasures 

Is the incubator plugged in 

Is there a power outage, or has a circuit breaker interrupted the 

power 

Is the key lock function turned OFF 

When starting operation: 

Does the chamber temperature match the set value 

Does the CO 2 gas density in the chamber match the set value 

(1) Is the secondary pressure for the gas regulator at the specified value 

of 0.03 MPa(G) (0.3 kgf/cm 2 (G), 4.3 psi(G)) 

(2) Is the gas tube properly connected 

During operation: 

Is the high limit alarm temperature set at least 1 C higher than the 

chamber set temperature 

Has the temperature setting been changed Has the outer door been 

left open for a long time Has a low-temperature object been placed in 

the chamber If any of these is the case, the alarm will be 

automatically cleared if you wait. 

Has the gas tube come loose, or is there a gas leak 

Has the CO 2 gas density setting been changed 

Is the gas cylinder empty Check the primary pressure of the gas 

cylinder once a week. (When the primary pressure is 3.8 MPa(G) (38 

kgf/cm 2 (G), 5.5 kpsi(G)) or lower, it is a sign that there is little gas 

remaining. Replace the cylinder soon.) 

Is the incubator operating beside the appliance that generates the 

electromagnetic wave 

Is the ambient temperature less than 5 C different from the set value 

for the chamber temperature 

Is the outer door closed with the inner door left open 

Is the secondary pressure for the gas regulator at the specified value 

of 0.03 MPa(G) (0.3 kgf/cm 2 (G), 4.3 psi(G)) 

Is the gas tube blocked 

Is there enough water in the humidifying pan (Be sure to use sterile 

distilled water.) 

Are the outer and inner doors being frequently opened and closed 

Check whether gas is leaking from connectors due to deterioration of 

the gas tube, or whether there may be any pinhole leaks. The gas tube 

is a replaceable part, and it is recommended that it be replaced once a 

year. 

Is the packing seal for the inner door defective 

Is the access hole open 

Is the ambient air environment around the incubator normal Is there a 

source of polluted gas in the vicinity 

51

TROUBLESHOOTING 

Symptom 

The CO 2 gas density is slow to 

recover. 

CO 2 gas is not being injected. 

The outer door does not open. 

H 2 O 2 decontamination cannot be 

performed. 

In H 2 O 2 decontamination cycle, 

”Err34:H2O2 Volume “ occurred. 

Items to check and countermeasures 

A HEPA filter is used for the incubator CO 2 gas piping. If gas 

density is slow to recover when the CO 2 gas pressure is normal, it 

is possible that the HEPA filter may be clogged. Contact our sales 

representative or agent. 

Is there little gas remaining in the CO 2 gas cylinder 

Is the secondary pressure for the gas regulator at the specified 

value of 0.03 MPa(G) (0.3 kgf/cm 2 (G), 4.3 psi(G)) 

Is the gas tube blocked 

The CO 2 control method for the incubator is the ON-OFF method. 

CO 2 gas is intermittently injected as the gas density in the chamber 

approaches the set value. Injections may be stopped for periods of 

approximately 15 seconds, but that is not an error. 

MCO-19AIC(UVH), or when the optional MCO-HL is installed: 

When the power switch is OFF, the electric lock is locked and the 

outer door does not open. Either turn ON the power switch or use 

the accessory unlock key to override the electric lock. 

During decontamination the outer door is electrically locked and 

does not open. 

If the MCO-19AIC(UV)/19AIC is being used, is the optional 

MCO-HL installed 

If the MCO-19AIC is being used, are the optional MCO-HL and 

MCO-19UVS installed 

Is the UV lamp burned out If the UV lamp is burned out, H 2 O 2 

decontamination will not be possible. 

Is the H 2 O 2 generator cable properly connected 

Has the entire bottle of the H 2 O 2 reagent been used 

Has the high limit temperature alarm been set to 50 C or higher 

Is the duct securely attached Attach the duct properly to the 4 

points hooks. (Fig. 1) 

Is the fan attached properly Remove the central spring at the 

center of the fan and confirm if the fan is pushed all the way to the 

motor shaft. Then, reinstall the central spring. (Fig. 2) 

Is the H 2 O 2 generator securely installed Set the 2 pins of the H 2 O 2 

generator in the 2 holes at the left bottom of the duct. (Fig. 3) 

1 Push the fan deeply. 

Fig. 1 

2 Install the central spring 

afterwards. 

3 Lightly turn the fan manually to 

make sure that it does not touch 

the rear panel. 

Fig. 2 

Fig. 3 

Note: If the problem still has not been solved after trying the above checks and countermeasures, or for 

any problems not covered here, contact our sales representative or agent. 

52

DISPOSAL OF UNIT 

When disposing of the CO 2 incubator, contact our sales representative or agent. 

WARNING 

The CO 2 incubator must be dismantled and disposed of by qualified personnel only. If the CO 2 

incubator is left where outsiders enter, it may result unexpected accident (for example, children to become 

locked inside). 

Before disposing the CO 2 incubator with biohazardous danger, decontaminate the CO 2 incubator to the 

extent possible by the user. 

53


Note: 

This symbol mark and recycle system apply only to EU countries. They 

do not applied to the countries in other areas of the world. 

Waste Electrical and Electronic Equipment (WEEE) Directive 2002/96/EC 

(English) 

This Panasonic product is designed and manufactured with high-quality materials and components that 

can be recycled and reused. 

This symbol means that the electrical and electronic equipment, at their end-of-life, should be disposed of 

separately from household wastes. 

Dispose of this product at your local community waste collection/recycling center. 

In the European Union there are separate collection systems for used electrical and electronic products. 

Please help us to conserve the environment we live in. 

(German) 

Ihr Panasonic Produkt wurde entworfen und hergestellt mit qualitativ hochwertigen Materialien und 

Komponenten, die recycelt und wiederverwendet werden können. 

Dieses Symbol bedeutet, daß elektrische und elektronische Geräte am Ende ihrer Nutzungsdauer von 

Hausmüll getrennt entsorgt werden sollen. 

Bitte entsorgen Sie dieses Gerät bei Ihrer örtlichen kommunalen Sammelstelle oder im Recycling Centre. 

In der Europäischen Union gibt es unterschiedliche Sammelsysteme für Elektrik- und Elektronikgeräte. 

Helfen Sie uns bitte, die Umwelt zu erhalten, in der wir leben! 

54

(French) 

Votre produit Panasonic est conçu et fabriqué avec des matèriels et des composants de qualité supérieure 

qui peuvent être recyclés et réutilisés. 

Ce symbole signifie que les équipements électriques et électroniques en fin de vie doivent être éliminés 

séparément des ordures ménagères. 

Nous vous prions donc de confier cet équipement à votre centre local de collecte/recyclage. 

Dans l’Union Européenne, il existe des systèmes sélectifs de collecte pour les produits électriques et 

électroniques usagés. 

Aidez-nous à conserver l’environnement dans lequel nous vivons ! 

Les machines ou appareils électriques et électroniques contiennent fréquemment des matières qui, si elles 

sont traitées ou éliminées de manière inappropriée, peuvent s’avérer potentiellement dangereuses pour la 

santé humaine et pour l’environnement. 

Cependant, ces matières sont nécessaires au bon fonctionnement de votre appareil ou de votre machine. 

Pour cette raison, il vous est demandé de ne pas vous débarrasser de votre appareil ou machine usagé 

avec vos ordures ménagères. 

(Spanish) 

Los productos Panasonic están diseñados y fabricados con materiales y componentes de alta calidad, 

que pueden ser reciclados y reutilizados. 

Este símbolo significa que el equipo eléctrico y electrónico, al final de su ciclo de vida, no se debe 

desechar con el resto de residuos domésticos. 

Por favor, deposite su viejo “televisor” en el punto de recogida de residuos o contacte con su 

administración local. 

En la Unión Europea existen sistemas de recogida específicos para residuos de aparatos eléctricos y 

electrónicos. 

Por favor, ayúdenos a conservar el medio ambiente! 

55


(Portuguese) 

O seu produto Panasonic foi concebido e produzido com materiais e componentes de alta qualidade que 

podem ser reciclados e reutilizados. 

Este símbolo significa que o equipamento eléctrico e electrónico no final da sua vida útil deverá ser 

descartado separadamente do seu lixo doméstico. 

Por favor, entregue este equipamento no seu ponto local de recolha/reciclagem. 

Na União Europeia existem sistemas de recolha separados para produtos eléctricos e electrónicos 

usados. 

Por favor, ajude-nos a conservar o ambiente em que vivemos! 

(Italian) 

Il vostro prodotto Panasonic è stato costruito da materiali e componenti di alta qualità, che sono 

riutilizzabili o riciclabili. 

Prodotti elettrici ed elettronici portando questo simbolo alla fine dell’uso devono essere smaltiti 

separatamente dai rifiuti casalinghi. 

Vi preghiamo di smaltire questo apparecchio al deposito comunale. 

Nell’Unione Europea esistono sistemi di raccolta differenziata per prodotti elettrici ed elettronici. 

Aiutateci a conservare l’ambiente in cui viviamo! 

56

(Dutch) 

Panasonic producten zijn ontwikkeld en gefabriceerd uit eerste kwaliteit materialen, de onderdelen kunnen 

worden gerecycled en weer worden gebruikt. 

Het symbool betekent dat de elektrische en elektronische onderdelen wanneer deze vernietigd gaan 

worden , dit separaat gebeurt van het normale huisafval. 

Zorg ervoor dat het verwijderen van de apparatuur bij de lokaal erkende instanties gaat gebeuren. 

In de Europese Unie wordt de gebruikte elektrische en elektronische apparatuur bij de daarvoor wettelijke 

instanties aangeboden. 

Alstublieft help allen mee om het milieu te beschermen. 

(Swedish) 

Din Panasonic produkt är designad och tillverkad av material och komponenter med hög kvalitet som kan 

återvinnas och återanvändas. 

Denna symbol betyder att elektriska och elektroniska produkter, efter slutanvändande, skall sorteras och 

lämnas separat från Ditt hushållsavfall. 

Vänligen, lämna denna produkt hos Din lokala mottagningstation för avfall/återvinningsstation. 

Inom den Europeiska Unionen finns det separata återvinningssystem för begagnade elektriska och 

elektroniska produkter. 

Vänligen, hjälp oss att bevara miljön vi lever i! 

57

AUTOMATIC CO 2 CYLINDER CHANGEOVER 

A gas auto changer (MCO-21GC) is available as an optional accessory. This kit switches the gas supply 

lines when one of the CO 2 gas cylinders becomes empty. 

Note: The installation of the MCO-21GC must be performed only by qualified service personnel. 

Install the MCO-21GC and then perform the following procedure. 

1. Connect 2 gas tubes to the connecting ports A and B for CO 2 gas pipe. The connecting ports A and B 

for CO 2 gas pipes are located on the left back of the incubator. (Refer to page 8). 

Note: Only the port A can be used when the MCO-21GC is not installed. There will be 2 ports, the ports A 

and B, after the MCO-21GC is installed. 

2. Connect a CO 2 gas cylinder equipped with a gas regulator to each gas tube. Refer to page 14 for 

information on connecting the gas cylinders. (“Gas cylinder A” is the CO 2 gas cylinder connected to the 

connecting port A for CO 2 gas pipe, and “gas cylinder B” is the CO 2 gas cylinder connected to the 

connecting port B for CO 2 gas pipe.) 

3. Open the valves of both gas cylinders. 

4. The current CO 2 gas supply line is displayed on the “Top Display”. 

(“A” and “B” will be displayed only when the MCO-21GC is installed. Nothing will be displayed if 

MCO-21GC is not installed.) 

Current CO 2 gas supply line 

1 T e m p 3 7 . 0 o C C O 2 5 . 0 % A B 

2 

3 

4 

5 

6 

7 

37.0 5.0 

S t a t u s 

O K 

5. The MCO-21GC detects that there is no more CO 2 gas in a cylinder when the CO 2 density in the 

chamber does not increase for a while even after opening the CO 2 gas valve in the incubator. It switches 

the gas supply line when it detects that there is no more gas. At that time, the indications of switching the 

gas cylinder are as follows: 

Message display field 

Display of gas supply 

The character for gas supply line 

When CO 2 gas supply line switches 

that is empty blinks. 

Err01: CO 2 Gas Empty is 

automatically (Only when MCO-21GC 

The gas supply line that is 

displayed. 

is installed.) 

currently being used is displayed 

in reverse video. 

●To silence the buzzer, press the buzzer key (BUZZER). The alarm condition is cleared and normal 

operation continues. 

58

6. When the gas cylinder A is switched to the gas cylinder B, remove gas cylinder A and replace it with 

new one. 

Note: Be careful when handling the empty CO 2 gas cylinder. Some gas may still be left in the cylinder. 

●When the gas cylinder B becomes empty, the line is switched to the gas cylinder A again. 

The MCO-21GC detects that there is no more CO 2 gas in a cylinder when the CO 2 density in the chamber 

does not increase for a while even after opening the CO 2 gas valve in the incubator. The supply line may 

also be switched for other reasons even though there is gas left in the cylinder: blocking or restricting of 

gas tube, reduction of CO 2 gas pressure, or improper opening of CO 2 gas cylinder. Always check the gas 

quantity in the cylinder before disconnecting it. 

● Use the following procedure to manual switch the line between CO 2 cylinders A and B (only when 

MCO-21GC is installed). This procedure shows switching the line from the gas cylinder B to the gas 

cylinder A as an example. 

1. From the “Top Display”, press the “Left Cursor Key” for 5 seconds. 

1 T e m p 3 7 . 0 o C C O 2 5 . 0 % A B 

2 

3 

4 

5 

6 

7 

37.0 5.0 

S t a t u s 

O K 

2. The CO 2 gas supply line will be switched from the gas cylinder B to the gas cylinder A. 

1 T e m p 3 7 . 0 o C C O 2 5 . 0 % A B 

2 

3 

4 

5 

6 

7 

37.0 5.0 

S t a t u s 

O K 

(When the line needs to be switched from the gas cylinder A to the gas cylinder B, press the “Right 

Cursor Key” for 5 seconds.) 

59

AUTOMATIC CO 2 DENSITY CALIBRATION 

To calibrate the CO 2 density, a STD gas auto calibration kit (MCO-SG) is available. By connecting a CO 2 

standard gas cylinder that has the density that is the same as the set CO 2 density (usually 5 % CO 2 ), the 

CO 2 sensor can be calibrated to maintain the accuracy of the CO 2 density display as necessary. 

● Setting the connected standard CO 2 gas density 



1 T e m p 3 7 . 0 o C C O 2 5 . 0 % 

2 

3 

4 

5 

6 

37.0 5.0 

S t a t u s 

O K 

M E N U 

S e t 

L o g 

T o o l s 

7 

2. Press the “Down Cursor Key” from the “Select Tools (1/2)” display to display the “Select Tools (2/2)” 

display, and then select “Standard Gas Setting”, press the menu key (MENU) to display the menu, select 

“OK”, and press the enter key (ENT). 

S e l e c t T o o l s ( 1 / 2 ) 






M E N U 

O K 

S v c 

C a n c e l 

D a t e 

T i m e 

S e l e c t T o o l s ( 2 / 2 ) 

S t a n d a r d G a s S e t t i n g 

S t a n d a r d G a s C a l i b r a t i o n 

M E N U 

O K 

S v c 

C a n c e l 

60

3. Input the CO 2 density of standard gas to be used, press the menu key (MENU) to display the menu, 


1 

3 

4 


M E N U 

C O 2 S T D G a s D e n s i t y 5 . 0 % 

O K 

% 

C a n c e l 

5 

6 

● Calibration method for CO 2 gas density 

1. Select “Standard Gas Calibration” from the “Select Tools (2/2)” display, press the menu key (MENU) to 

display the menu, select “OK”, and press the enter key (ENT). 

S e l e c t T o o l s ( 2 / 2 ) 


S t a n d a r d G a s C a l i b r a t i o n M E N U 

O K 

S v c 

C a n c e l 

2. The system check will start. 

1 

3 

4 

5 

S t a n d a r d G a s C a l i b r a t i o n S t e p : 1 

S t d G a s C a l i b S y s t e m C h e c k ・・・・・ 

C O 2 : 0 . 0 % 

S T G : 5 . 0 % 

6 

61

AUTOMATIC CO 2 DENSITY CALIBRATION 

3. If the system is normal, the following display will appear. Select “OK” and press the enter key (ENT) to 

start calibrating the CO 2 density. After calibration, return to normal cultivating operation. 

1 

3 

4 

5 

S t a n d a r d G a s C a l i b r a t i o n 

R e a d y t o S t a r t 

M E N U 

O K 

C a n c e l 

C O 2 : 0 . 0 % 

S T G : 5 . 0 % 

6 

1 

3 

4 

5 

S t a n d a r d G a s C a l i b r a t i o n S t e p : 3 

S t d G a s C a l i b r a t i n g ・・・・・ 

C O 2 : 4 . 8 % 

S T G : 5 . 0 % 

6 

1 T e m p 3 7 . 0 o 

C C O 2 5 . 0 % 

2 

3 

4 

5 

6 

7 

37.0 5.0 

S t a t u s 

O K 

62

STACKING INCUBATORS 

Use the following procedure to stack incubators. This work is potentially dangerous, so contact our sales 

representative or agent. 

CAUTION 

Select a floor that is strong enough to support the stacked incubators. 

Never stack 3 or more incubators. Doing so is dangerous. 

1. Take out 2 stacking plates A, 2 stacking plates B, 4 protective stickers, and 4 screws from the 

accessory bag. 

Note: 

When stacking 2 incubators, use 2 stacking plates (A and B) for each one. Only one pair of stacking 

plates is provided per an incubator, so take out the stacking plates (A and B) from both incubators. 

Similarly only 2 protective stickers are provided per an incubator 

2. Unplug both of the incubators that are to be stacked. 

3. Remove the caps at the 2 places in the front of the top surface of the incubator that is to be on the 

bottom, attach the protective stickers to the 4 corners on the top surface of the incubator that is to be on 

the bottom. (Fig. A) 

4. Secure stacking plate A with the screws that are provided. 

5. Remove the front panel (5 screws) from the incubator that is to be on the top. 

6. Place the upper incubator on top of the lower one, being careful not to pinch your fingers. 

7. Make the lower incubator level by adjusting the leveling feet, and then level the upper incubator in the 

same way. 

8. Secure the stacking plate A fixed to the lower incubator to the upper incubator with the accessory 

screws. 

9. Remove from the back of the lower incubator the 2 hooks (4 screws) and remove from the upper 

incubator the 2 screws on the lower rear panel. 

10. Use the 6 screws that were removed in step 9 to secure stacking plate B to the rear panels of the 

upper and lower incubators. 

11. Replace the front panel (5 screws) to the upper incubator. 

12. Use the hooks on the back of the upper incubator to prevent the incubators from falling over. 

63

STACKING INCUBATORS 

Front panel 

Stacking plate B 

Front 

Stacking plate A 

Protective sticker 

Hook 

Fig. A 

64

SPECIFICATIONS 

Product name 

External dimensions 

Internal dimensions 

Interior volume 

Exterior 

Interior 

Outer door 

Inner door 

Trays 

Access port 

Insulation 

Heating system 

Heater 

Humidifying system 

Temperature controller 

Temperature display 

CO 2 controller 

CO 2 density display 

Air circulation 

Air filter 

CO 2 incubator 

MCO-19AIC 


MCO-19AIC(UV) 


MCO-19AIC(UVH) 

W620 mm x D710 mm x H900 mm (W24.4 inch x D28.0 inch x H35.4 inch) 


170 L (6.00 cu.ft.) 

Painted steel 

Stainless steel containing copper 

Painted steel 

Tempered glass 

4 trays made of stainless steel containing copper 


Maximum load: 7 kg/tray 

Inner diameter: 30 mm (1.18 inch), On the back side 

Rigid polyurethane foamed-in place 

DHA system (heater jacket + air jacket system) 

314 W 

Natural evaporation with humidifying pan 

PID control system 

Digital display 

PID control system 

Digital display 

Fan assisted 

0.3 m, Efficiency: 99.97 % or higher 

UV lamp ----- 4W x 1 (ozone-free emission) 


Alarms 

Remote alarm contacts 

CO 2 inlet connection 

CO 2 inlet pressure 

Weight 

Accessories 

Optical 

Automatic set temperature alarm, Automatic set CO 2 density alarm, 

High limit temperature alarm, CO 2 gas, various sensor/heater alarms 

Allowable contact capacity: DC 30 V, 2 A 

4 mm to 6 mm (0.157 inch to 0.236 inch) diameter tube can be connected 

0.03 MPa(G) (0.3 kgf/cm 2 (G), 4.3 psi(G)) 

93 kg 

4 trays, 4 sets of tray supports, 1 gas tube, 1 humidifying pan, 

1 pair of stacking plates A and B, 2 protective stickers, 2 tube bands 

65

SPECIFICATIONS 

Product name 


MCO-19AIC 

UV system set 

(MCO-19UVS) 

H 2 O 2 generator (MCO-HP) 

H 2 O 2 decon set (MCO-HL) 





H 2 O 2 generator and 

H 2 O 2 decon set are 

standard equipment 

UV system set is a standard equipment. 

H 2 O 2 reagent (MCO-H2O2) 

STD gas auto calibration kit (MCO-SG) 

Gas regulator (MCO-100L) 

Optional accessories 

Tray (MCO-47ST, includes 2 tray supports) 

Half tray (MCO-25ST), Gas auto changer (MCO-21GC) 

Roller base (MCO-18RB), UV replacement kit (MCO-20UV) 

Stacking plate (MCO-18SB, when stacking on the MCO-175) 

Stacking plate (MCO-21SB, when stacking on the MCO-20AIC) 

Interface board (MTR-L03), Interface board (MTR-480) 

Data acquisition system (MTR-5000) 

interface board (MCO-420MA) 

Note: Refer to the updated catalog when ordering an optional component. 

Designs and specifications are subject to change without notice. 

PERFORMANCE 

Product name 

CO 2 Incubator MCO-19AIC 



Model No. 

MCO-19AIC-PT 

MCO-19AICUV-PT 

MCO-19AICUVH-PT 

MCO-19AIC-PA 

MCO-19AICUV-PA 

MCO-19AICUVH-PA 

MCO-19AIC-PK 

MCO-19AICUV-PK 

MCO-19AICUVH-PK 

Temperature control range Ambient temperature+5 o C to 50 o C (ambient temperature: 5 o C to 35 o C) 

Temperature distribution 

Temperature variation 

±0.25 o C (ambient temperature: 25 o C, setting: 37 o C, CO 2 : 5 %, no load) 

±0.1 o C (ambient temperature: 25 o C, setting: 37 o C, CO 2 : 5 %, no load) 

CO 2 control range 0 % to 20 % 

CO 2 variation 

Chamber humidity 

Applicable environment 

condition 

Noise level 

±0.15 % (ambient temperature: 25 o C, setting: 37 o C, CO 2 : 5 %, no load) 

95 %R.H.±5 %R.H. 

Temperature: 5 o C to 35 o C, Humidity: 80 %R.H. max. 

(The designed performance may not be obtained 

If the ambient temperature is equal or less than 15 o C) 

30 dB (A scale) 

Power consumption Max. 350 W Max. 310 W 

Heat emission 

Max. 1,120 kJ/h 

Rated voltage, frequency 110 V to 120 V, 60 Hz 220 V, 60 Hz 

Amperage Max. 2.8 A Max. 1.4 A 

Note: The unit with CE mark complies with EC directives. 

Based on our measuring method. 

66

CAUTION 

Please fill in this form before servicing. 

Hand over this form to the service engineer to keep for his and your safety. 

Safety check sheet 

1. Unit contents : 

Risk of infection: 

Risk of toxicity: 

Risk from radioactive sources: 

□Yes 

□Yes 

□Yes 

□No 

□No 

□No 

(List all potentially hazardous materials that have been stored in this unit.) 

Notes : 

2. Contamination of the unit 

Unit interior 

No contamination 

Decontaminated 

Contaminated 

Others: 

□Yes 

□Yes 

□Yes 

□No 

□No 

□No 

3. Instructions for safe repair/maintenance of the unit 

a) The unit is safe to work on 

b) There is some danger (see below) 

□Yes 

□Yes 

□No 

□No 

Procedure to be adhered to in order to reduce safety risk indicated in b) below. 

Date : 

Signature : 

Address, Division : 

Telephone : 

Product name : 


Model No. 

MCO- 

Serial number : Date of Installation : 

Please decontaminate the unit yourself before calling the service engineer. 

67

1-1-1, Sakata Oizumi-Machi Ora-Gun, Gunma 370-0596, Japan 

© Panasonic Healthcare Co., Ltd. 2012 

Printed in Japan 

7FB6P15152900 

S0412-1042

MCO-19AIC MCO-19AICUV MCO-19AICUVH Series - Panasonic ...

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