Analysis of Nitrate Nitrogen (NO3-) in Water by the EPA Approved ...
Analysis of Nitrate Nitrogen (NO3-) in Water by the EPA Approved ...
Analysis of Nitrate Nitrogen (NO3-) in Water by the EPA Approved ...
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Results<br />
Figure 2 shows <strong>the</strong> s<strong>of</strong>tware screen for <strong>the</strong> standard solution<br />
data collection. Full spectra <strong>of</strong> all standards are displayed<br />
<strong>in</strong> <strong>the</strong> Standard Spectrum w<strong>in</strong>dow (upper left). Details <strong>of</strong><br />
<strong>the</strong> standards used and <strong>the</strong>ir measured absorbance values<br />
at <strong>the</strong> analytical wavelength (410 nm) are shown at <strong>the</strong><br />
bottom <strong>of</strong> <strong>the</strong> screen and a calibration curve is plotted<br />
(upper right). The equation <strong>of</strong> <strong>the</strong> l<strong>in</strong>e <strong>of</strong> best fit is<br />
calculated <strong>by</strong> <strong>the</strong> least squares method and displayed<br />
below <strong>the</strong> Standard Spectrum.<br />
A sample conta<strong>in</strong><strong>in</strong>g an unknown amount <strong>of</strong> nitrate<br />
was prepared accord<strong>in</strong>g to <strong>the</strong> procedure and measured <strong>in</strong><br />
duplicate. The results were displayed as shown <strong>in</strong> Figure 3.<br />
The concentration <strong>of</strong> <strong>the</strong> sample was found to be 0.23 ppm.<br />
Figure 2: Standard solution data<br />
Conclusion<br />
In this experiment quantitative analysis <strong>of</strong> nitrate nitrogen<br />
(NO - 3 ) <strong>in</strong> water was successfully performed with <strong>the</strong><br />
Evolution Array spectrophotometer and VISIONcollect<br />
s<strong>of</strong>tware. Rapid acquisition <strong>of</strong> full-range spectra and<br />
excellent <strong>in</strong>strument sensitivity made <strong>the</strong> analysis quick,<br />
accurate and reproducible.<br />
References<br />
1. Electronic: http://www.epa.gov/waterscience/methods/method/files/352_1.pdf .<br />
Date acquired 12-12-2009.<br />
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