CANAG ProGRP EIA - PRODUCT SHEET - Fujirebio Diagnostics, Inc.

PRODUCT INFORMATION
CanAg ProGRP EIA
The CanAg ProGRP EIA Kit is a solid phase, one-step immunoassay for the quantitative
determination of ProGRP. ProGRP has been shown to have a high sensitivity and
specificity in serum for SCLC. Data indicates that it may be a promising biomarker for
the diagnosis and monitoring of SCLC and for use in discriminating NSCLC from SCLC.
Simple Assay Format
Easy to Use one-step immunoassay
Pre-diluted calibrators
Room temperature incubation
“ A new promising tool for
the management of
Small Cell Lung Cancer (SCLC)”
SpecificationS ProDuct no 220-85
Results within: 3 hours, one step procedure
Detection limit: < 2 ng/L
Measuring range: 2–1000 ng/L
Sample volume: 50 µL
Stability:
18 months at 4° C
Calibrator range: 0-1000 ng/L
Incubation temp: 20–25° C
Detection:
450 nm
Shedding light on a dark
and dangerous disease
For Research Use Only
global leader

CanAg ProGRP EIA
The neuropeptide hormone Gastrin Releasing Peptide (GRP), the human counterpart of amphibian
bombesin, is synthesised as a prohormone, proGRP. ProGRP exists in three different variants
with 115, 118 or 125 amino acids, respectively. The region proGRP31-98 is common
for all three variants, and antibodies towards this region have been used for development
of assays for determination of proGRP. Increased ProGRP levels have been detected in
small cell lung carcinoma (SCLC).
CanAg ProGRP EIA, ng/L
1000
900
800
700
600
500
400
300
200
100
0
CanAg ProGRP EIA vs Competitor ProGRP EIA
0 100 200 300 400 500 600 700 800 900 1000
Competitor ProGRP EIA, ng/L
Fujirebio Diagnostics Inc.
201 Great Valley Parkway
Malvern, PA 19355-1307
USA
+1 610 240 3800 phone
+1 610 240 3803 fax
info@fdi.com e-mail
www.fdi.com website
Fujirebio Diagnostics AB
Majnabbeterminalen
SE-414 55 Göteborg
Sweden
+46 31 85 70 30 phone
+46 31 85 70 40 fax
info@fdab.com e-mail
www.fdab.com website
Correlation between ProGRP EIA and competitor assay. The linear regression equation was
y = 1,03x - 0,79 and the coefficient of correlation was r 2 = 0,98, n = 63.
The CanAg ProGRP EIA is a one step sandwich EIA performed in microwells coated with
streptavidin. ProGRP is measured in 50 µL of serum, which is mixed with antibody solution
and incubated at room temperature for 2 hours. The antibody solution consists of peroxidase
labelled E146 mouse monoclonal antibody, reactive against proGRP aa 48-53, and
biotinylated affinity purified goat polyclonal antibody specific for proGRP 31-98.
3.5
3.0
2.5
10
9
8
7
OD450
2.0
6
5
1.5
4
1.0
OD450 nm
CV(%)
3
2
0.5
1
0.0
0
0 200 400 600 800 1000 1200
ProGRP ng/L
CV%
Dose-response and precision profile
A typical calibration curve and precision profile
obtained with the CanAg ProGRP EIA kit is
shown below. The precision profile is based on
random pipetting of calibrators into one microplate,
n=8.
Progrp EIA, June 2007, r1