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Vomiting & Diarrhoea in a nursing home - Rcpa.tv

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The history is certa<strong>in</strong>ly suggestive of an <strong>in</strong>fective process,<br />

and both giardia and cryptosporidia should also be<br />

sought. Choose the best answer below:<br />

A. Giardia are usually present <strong>in</strong> large numbers <strong>in</strong> faeces, so either microscopy or<br />

direct antigen detection are appropriate. Cryptosporidia though are usually<br />

present <strong>in</strong> smaller numbers and direct antigen detection is a more sensitive,<br />

but a less specific diagnostic assay.<br />

B. A comb<strong>in</strong>ed iron haemotoxyl<strong>in</strong> and trichrome sta<strong>in</strong> of a faecal smear provides<br />

the most sensitive detection method for giardia and cryptosporidium<br />

detection. This method is more labour <strong>in</strong>tensive and is limited by a higher<br />

threshold of organism detection than the PCR or immuno-detection methods,<br />

but those methods also have imperfections.<br />

A<br />

B

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