Vomiting & Diarrhoea in a nursing home - Rcpa.tv

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The history is certainly suggestive of an infective process, and both giardia and cryptosporidia should also be sought. Choose the best answer below: A. Giardia are usually present in large numbers in faeces, so either microscopy or direct antigen detection are appropriate. Cryptosporidia though are usually present in smaller numbers and direct antigen detection is a more sensitive, but a less specific diagnostic assay. B. A combined iron haemotoxylin and trichrome stain of a faecal smear provides the most sensitive detection method for giardia and cryptosporidium detection. This method is more labour intensive and is limited by a higher threshold of organism detection than the PCR or immuno-detection methods, but those methods also have imperfections. A B
Giardia & Cryptosporidium detection A controversial area, different publications (read them below), have claimed different sensitivities for the differing methods. Essentially light microscopy has threshold of detection of 500,000 cysts/ml, and immuno-detection can miss strains, of which a number of different genetic types exist JCM paper JCM paper JCM paper
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Vomiting & Diarrhoea in a nursing home - Rcpa.tv

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