Model Answers Microbiology Written examinations 2007 - RCPA
Model Answers Microbiology Written examinations 2007 - RCPA
Model Answers Microbiology Written examinations 2007 - RCPA
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Eyes<br />
Corneal scrapings – fungal keratitis<br />
Aqueous vitreous humour<br />
Tissue biopsies of any kind<br />
E.g. disseminated disease<br />
Brain<br />
Adrenals<br />
Thyroid<br />
Blood cultures<br />
Candidemias or fungemias<br />
How and why<br />
Media<br />
SAB (+ chlor, gent) 25-30C<br />
SD (SAB + cycoheximide) = MYCOSEL or (actidione) + (chlo + gent) 25-<br />
30C<br />
DTM = (chlo + gent + cycl) 25-30C<br />
SAB without antibiotics (may use for tissues from sterile sites)<br />
Chromogenic agar<br />
Dixons or overlay with olive oil for M.furfur from blood and occas skin but<br />
generally not indicated as microscopy sufficient from skin – 37C<br />
Set up in tubes to prevent drying as media held 3-4 weeks<br />
Avoid opening lids unless growth to prevent contamination<br />
Sporing for microscopy<br />
OA<br />
SACHs<br />
PDA – slide cultures/species etc<br />
CMA<br />
Basic techniques<br />
Macroscopic description of colonies<br />
Colour – surface reverse etc<br />
Appearance<br />
Microscopy from sample<br />
Hyphae – hyaline<br />
Hyphae – pigmented – T.nigra and other demateaciuos fungi<br />
Spores<br />
Yeast cells<br />
Pseudohyphae<br />
Copper pennies (Chromo) – SAB 28<br />
Spaghetti meat ball – M.f<br />
Microscopy of colonies<br />
Microconidia<br />
Macrocondidia<br />
Chlamydospores<br />
Other techniques<br />
Dimorphism demonstration: Mycelial conversion at 28 to Yeast at 37<br />
Exoantigen test – Histoplasmosis<br />
Differential temps of incubation<br />
Canavanine to speciate Cryptococcus – gattii v neoformans: association with<br />
HIV and prognosis
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