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Model Answers Microbiology Written examinations 2007 - RCPA

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Organism identification<br />

Bacteria<br />

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Fungi<br />

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Viruses<br />

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Choice of media, conditions of culture<br />

Non-cultural detection of bacterial infection<br />

o Ag detection<br />

o PCR<br />

Tests for tuberculosis<br />

o AFB staining<br />

o Culture<br />

o PCR (discuss methods)<br />

o (Chemical tests eg. adenine deaminase)<br />

Tests for syphilis<br />

o Especially critical evaluation for evidence of neurosyphilis<br />

Tests for Cryptococcus<br />

o Testing and significance of Ag detection<br />

o Serial CSF testing in evaluation of success of therapy<br />

Testing for other fungi e.g. dimorphic fungi<br />

CNS aspergillosis and other invasive mycoses<br />

Culture<br />

Noncultural methods especially PCRs<br />

Test selection for meningitis, encephalitis<br />

Special testing:<br />

o HIV – viral load<br />

o Testing in travellers e.g. arboviruses<br />

o Tests in immune-compromised patients e.g. EBV infection, JC virus<br />

o Testing for SSPE<br />

Parasites<br />

Diagnosis of CNS toxoplasmosis, cysticercosis, amoebic meningoencephalitis,<br />

African trypanosomiasis, angiostrongyliasis etc<br />

Prions<br />

Safety considerations

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