The Influence of Oxidative Stress, Carcinogens and Cloning on DNA ...

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CE-LIF Capillary electrophoresis with laser induced fluorescence detector CE-UV Capillary electrophoresis with ultraviolet detection CGE Capillary gel electrophoresis CEC Capillary electrochromatography CH2CL2 methylene chloride CHN Chinese Herbs nephropathy CIEF Capillary isoelectric focusing CITP Capillary isotachophoresis cm Centimeters COBRA Combined bisulphite restriction analysis CpG-Isl<strong>and</strong> 5’-CG-3’-dinucleotides CT-DNA Calf thymus-DNA CZE Capillary zone electrophoresis dAMP (or 3’-dAMP) 2’-deoxyadenosine-3’-monophosphate dA-AA I,II 7-(2’-deoxyadenosine-N6-yl)-aristolactam I,II dC-AAII 7-(2’-deoxycytosine-N6-yl)-aristolactam II dG-AA 7-(2’-deoxyguanosine-N2-yl)-aristolactam dCMP (or 3’-dCMP) 2’-deoxycytidine-3’-monophosphate dG 2’-deoxyguanosine dGMP (or 3’-dGMP) 2’-deoxyguanosine-3’-monophosphate dTMP (or 3’-dTMP) Thymidine-3’-monophosphate Da Daltons DNA Deoxyribonucleic acid DNase I Deoxyribonuclease I Dnmt DNA-methyl transferase DZ Dizygotic twins e.g. Example EDC 1-ethyl-3-(3’-N,N’-dimethylaminopropyl)-carbodiimide hydrochloride EDTA Ethylene diamine tetraacetic acid ESI-MS Electrospray ionisation mass spectrometry EtOH Ethanol f Corrrection factor 2
FDA Food <strong>and</strong> drug administration Fe 2+ Fig Ferrous ion Figure G Guanine h Hours H2O Water H2O2 Hydrogen peroxide HAAs Heterocyclic aromatic amines HBO Hyperbaric oxygen HC Holstein clones HCL Hydrochloric acid HEPES N-(2-hydroxyethyl)-piperazine-N’-2-ethanesulphonic acid Hpa II Hpa II Methyl transferase HPLC High performance liquid chromatography HPLC-ESI-MS High performance liquid chromatography with electrospray ionisation mass spectrometry HPLC-MS High performance liquid chromatography with mass spectrometry HPLC -UV High performance liquid chromatography with ultraviolet detection Hupki Human p53 knock in ICSI Intracytoplasmic sperm injection ID Inner diameter ITP Isotachophoresis IVF In vitro-Fertilisation kV Kilovolts L Liter (s) LD Length <strong>of</strong> capillary to the detector LIF Laser induced fluorescence LOS Large Offspring Syndrome LT Total capillary length mbar Millibar MEKC Micellar electrokinetic chromatography 3
Page 1 and 2: The Influe
Page 3 and 4: The work at h<stro
Page 5 and 6: First, I feel always grateful to Al
Page 7: I would like to extend my thanks to
Page 11: The following acro
Page 15: RIA Radioimmunoassay ROS Reactive o
Page 18 and 19: Index 3.1 Spectrophotometeric deter
Page 20 and 21: Index 5.2.4.2 Dialysis of</
Page 22 and 23: Introduction 1.1.2 DNA methylation
Page 24 and 25: Introduction 1.2 The</stron
Page 26 and 27: Introduction Figure 4: Model for me
Page 28 and 29: Introduction Cancer is classified i
Page 30 and 31: Introduction DNA methylation Gene h
Page 32 and 33: Introduction 1.2.7.1 Clonin
Page 34 and 35: Introduction 1.2.8 Twins an
Page 36 and 37: Introduction chemicals, alkylating
Page 38 and 39: Introduction AP sites are not only
Page 40 and 41: Introduction 1.3.2.1 Oxidat
Page 42 and 43: Introduction Finally, flavo- <stron
Page 44 and 45: Introduction chromatography with el
Page 46 and 47: Introduction One of</strong
Page 48 and 49: Introduction facturing of</
Page 50 and 51: Introduction 320) ; but due to its
Page 52 and 53: Introduction HO O O Figure 15: Mech
Page 54 and 55: Introduction - Methylation Sensitiv
Page 56 and 57: Introduction 5‘ 5‘ 5 Figure 18:
Page 58 and 59: Introduction - Radioactive-mediated
Page 60 and 61: Aim and objectives
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Summary 4 Summary DNA methylation i
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Summary The same s
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Experimental part Sodium tetrapheny
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Experimental part � Sodium tetrab
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Experimental part framework was fas
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Experimental part propanol
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Experimental part 12.5 mU/µL SPD)
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Experimental part Condition Value D
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Experimental part 5.4 Instrumentati
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Experimental part Graduated cylinde
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Experimental part 3-Nitrobenzanthro
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Appendix In-vitro: Denoting a react
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Appendix Laboratory Document Hydrol
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Appendix BODIPY and</strong
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Appendix Description of</st
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Appendix Transmission [%] 120 100 8
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Appendix Figure 62: Mass spectrum E
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List of figures Fi
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List of tables 8 L
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Literature Literature 1. Egger, G.;
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Literature 41. Bestor, T.H.; "<stro
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Literature 78. Venitt, S.; "Mechani
Page 180 and 181:
Literature 113. Sinclair, K.D.; Mce
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Literature 148. Kadan-Lottick, N.S.
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Literature cyte DNA by gas chromato
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Literature 221. Mirand</str
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Literature 257. Lingaraju, G.M.; Sa
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Literature different tissues <stron
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Literature 324. Vishwanath, B.S.; F
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Literature 359. Wei, L.; Li, R.K.;
Page 196 and 197:
Literature 394. Ramsahoye, B.H.; "M
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Literature 431. Wirtz, M.; Schumann
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Literature 467. Cornelius, M.; Wort
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Literature 502. Issa, J.P.J.; Ottav
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Literature 536. Pinheiro, J. <stron
Page 206:
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