The Influence of Oxidative Stress, Carcinogens and Cloning on DNA ...

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I would like to thank Matthias Albers for his conducting mass spectrometry measurements <strong>and</strong> sharing his expertise in MS data interpretation. My warmest thanks are to Stephan Stelljes for his help in performing some measurements for this work. My special thanks to Janine Bursa for her help in translation <strong>of</strong> a report for my work from English to German. I am very thankful to Ralf Radon for helping me installing s<strong>of</strong>tware, creating backups <strong>of</strong> my work <strong>and</strong> solving any technical problems throughout the work. I gratefully acknowledge Dr. Michaela Wirtz for some discussions at the beginning <strong>of</strong> my work that helped me to improve my knowledge <strong>of</strong> the topic. My warm thanks for the friendly cooperation <strong>of</strong> Dr. Beatrice de Montera at the “Laboratoire de Biologie du Développement et Reproduction”, France for preparing the clone samples <strong>and</strong> helping me underst<strong>and</strong> the ideas in this area. I would like to extend my thanks for her continuous contact, helpful comments <strong>and</strong> valuable discussions, as well as the nice time we spent together in Germany <strong>and</strong> France. Many thanks are to the friendly cooperation <strong>of</strong> Pr<strong>of</strong>. Dr. Sigrid Müller <strong>and</strong> his coworkers, at the Molecular Animal Breeding <strong>and</strong> Biotechnology, <strong>and</strong> Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU in Munich, Germany. My deep thanks to Fabian Scheipel, LMU in Munich, Germany for performing the statistical analysis. I also appreciate the sincere cooperation <strong>of</strong> Dr. Volker Arlt at the institute <strong>of</strong> Cancer Research, Section <strong>of</strong> Molecular Carcinogenesis in London, <strong>and</strong> his helpful contact It is my pleasure to thank Pr<strong>of</strong>. Zongwei Cai <strong>and</strong> his coworkers, at the department <strong>of</strong> Chemistry, Hong Kong Baptist University in Hong Kong, for the supply <strong>of</strong> in-vivo samples <strong>and</strong> the cooperation <strong>of</strong> his group in helping me to achieve part <strong>of</strong> this work. It is my pleasure to show my gratitude to my former <strong>and</strong> current colleagues: Dr. Rene Mönnike, Nils Wien<strong>and</strong>, Ann-Kathrin Galle, Sabrina Laun <strong>and</strong> Roman Dück for their help <strong>and</strong> interest. My thanks are to Ms. Waltraud Schiewek for her administrative help. I would like to thank the Teamwork <strong>of</strong> ZGS (Zentrum für Graduiertenstudien) at the University <strong>of</strong> Wuppertal: Dr. Janine Hauthal, Dilek Gürsoy <strong>and</strong> Denise Wulde for their help through their program for leveling up the graduation conditions for graduate students <strong>and</strong> the nice multicultural time I spent with them. 2
I would like to extend my thanks to Pr<strong>of</strong>. Dr. Wolfgang Schuhmann at the department <strong>of</strong> Electroanalytik <strong>and</strong> Sensorik, Ruhr Universität Bochum for his kind hospitality in accepting me to work in his group in the first semester in my study in Germany; without his acceptance, I would not have been able to conduct my research in Germany. I obtained new experience in his research area. My sincere appreciation is to Egyptian mission <strong>and</strong> DAAD (German Academic Exchange Service) for the financial support during my stay in Germany. In particular, my sincere thanks to DAAD for giving me the opportunity to pursue my doctoral research in Germany. My great appreciation is to Pr<strong>of</strong>. Dr. Galal El-Din El-Gemeey, director <strong>of</strong> Egyptian missions <strong>and</strong> culture <strong>of</strong>fice in Berlin, for his valuable advices <strong>and</strong> to my country reference, Mona Ayoub, DAAD <strong>of</strong>fice in Cairo, for her kind help <strong>and</strong> e-mail contact as well as to my regional reference, Margret Leopold, DAAD <strong>of</strong>fice in Bonn, for her advice <strong>and</strong> kind support during my stay in Germany My cordial thanks to my friends in Egypt who keep contact with me by phone <strong>and</strong> emails <strong>and</strong> whose love <strong>and</strong> care enabled me to achieve this work. I deeply value their friendship. Also, I greatly appreciate my friends in Germany who helped me to overcome the homesickness that I had in the beginning <strong>of</strong> my stay. I am also deeply grateful to my aunt, Pr<strong>of</strong>. Dr. Mervat Moussa, Faculty <strong>of</strong> Dentistry, Cairo University, who supported me in all stages <strong>of</strong> the dissertation through her revisions to many reports <strong>and</strong> letters that had to be admitted to the financial organizations <strong>and</strong> her continuous encouragement. I am grateful for her constructive suggestions <strong>and</strong> for making available to me her experience in the research field, specifically in conducting my research away from my home country. I would like to express my special thanks to my fiancé Ali, whose enthusiastic support <strong>and</strong> care has encouraged me to overcome the difficulties at the end stage <strong>of</strong> the doctoral thesis. Finally, I would like to <strong>of</strong>fer my great thanks to my father <strong>and</strong> my mother, to whom this dissertation is dedicated, for their patience, underst<strong>and</strong>ing, encouragement <strong>and</strong> effective support during the whole work. <strong>The</strong>ir love <strong>and</strong> concern helped me to achieve my own goals. 3
Page 1 and 2: The Influe
Page 3 and 4: The work at h<stro
Page 5: First, I feel always grateful to Al
Page 11 and 12: The following acro
Page 13 and 14: FDA Food and drug
Page 15: RIA Radioimmunoassay ROS Reactive o
Page 18 and 19: Index 3.1 Spectrophotometeric deter
Page 20 and 21: Index 5.2.4.2 Dialysis of</
Page 22 and 23: Introduction 1.1.2 DNA methylation
Page 24 and 25: Introduction 1.2 The</stron
Page 26 and 27: Introduction Figure 4: Model for me
Page 28 and 29: Introduction Cancer is classified i
Page 30 and 31: Introduction DNA methylation Gene h
Page 32 and 33: Introduction 1.2.7.1 Clonin
Page 34 and 35: Introduction 1.2.8 Twins an
Page 36 and 37: Introduction chemicals, alkylating
Page 38 and 39: Introduction AP sites are not only
Page 40 and 41: Introduction 1.3.2.1 Oxidat
Page 42 and 43: Introduction Finally, flavo- <stron
Page 44 and 45: Introduction chromatography with el
Page 46 and 47: Introduction One of</strong
Page 48 and 49: Introduction facturing of</
Page 50 and 51: Introduction 320) ; but due to its
Page 52 and 53: Introduction HO O O Figure 15: Mech
Page 54 and 55: Introduction - Methylation Sensitiv
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Introduction 5‘ 5‘ 5 Figure 18:
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Introduction - Radioactive-mediated
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Aim and objectives
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Summary 4 Summary DNA methylation i
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Summary The same s
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Experimental part Sodium tetrapheny
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Experimental part � Sodium tetrab
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Experimental part framework was fas
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Experimental part propanol
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Experimental part 12.5 mU/µL SPD)
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Experimental part Condition Value D
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Experimental part 5.4 Instrumentati
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Experimental part Graduated cylinde
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Experimental part 3-Nitrobenzanthro
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Appendix In-vitro: Denoting a react
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Appendix Laboratory Document Hydrol
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Appendix BODIPY and</strong
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Appendix Description of</st
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Appendix Transmission [%] 120 100 8
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Appendix Figure 62: Mass spectrum E
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List of figures Fi
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List of tables 8 L
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Literature Literature 1. Egger, G.;
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Literature 41. Bestor, T.H.; "<stro
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Literature 78. Venitt, S.; "Mechani
Page 180 and 181:
Literature 113. Sinclair, K.D.; Mce
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Literature 148. Kadan-Lottick, N.S.
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Literature cyte DNA by gas chromato
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Literature 221. Mirand</str
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Literature 257. Lingaraju, G.M.; Sa
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Literature different tissues <stron
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Literature 324. Vishwanath, B.S.; F
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Literature 359. Wei, L.; Li, R.K.;
Page 196 and 197:
Literature 394. Ramsahoye, B.H.; "M
Page 198 and 199:
Literature 431. Wirtz, M.; Schumann
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Literature 467. Cornelius, M.; Wort
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Literature 502. Issa, J.P.J.; Ottav
Page 204 and 205:
Literature 536. Pinheiro, J. <stron
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