The Influence of Oxidative Stress, Carcinogens and Cloning on DNA ...

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MEKC-LIF Micellar electrokinetic chromatography with laser induced fluorescence detector MeOH Methanol mL Milliliter(s) mm Millimeter MN Micrococcal nuclease MS Mass spectrometry MSREs Methylation sensitive restriction endonuclease mW Milliwatt MZ Monozygotic twins m/z Mass to charge ratio N.B. Nota bene Na2HPO4 Disodium hydrogen phosphate NaH2PO4 Sodium dihydrogen phosphate NaHCO3 Sodium bicarbonate NaOH Sodium hydroxide Na2SO3 Sodium sulphite ng Nanogram nL Nanoliter(s) nm nanometer NADPH-oxidase Nicotinamide adenine dinucleotide phosphate oxidase NER Nucleotide excision repair OH . Hydroxyl free radical Oxygen O2 O2 -. Superoxide ion O3 Ozone OPSL Optically pumped semiconductor laser P Pressure PA Peak area PAEKI Pressure assisted electrokinetic injection PAHs Polycyclic aromatic hydrocarbons PCR Polymerase chain reaction psi Pound per square inch RFU Relative fluorescence unit 4
RIA Radioimmunoassay ROS Reactive oxygen species RP Reversed phase Rpm Revolutions per minute RT Room temperature R.t. Retention time SAM S-adenosyl methionine SC Simmental clones SCNT Somatic cell nuclear transfer S.D. St<strong>and</strong>ard deviation SDS Sodium dodecyl sulphate s Seconds s.e.m. St<strong>and</strong>ard error <strong>of</strong> the mean S.I. Small intestine S/N Signal to noise ratio SPD Spleen phosphodiesterase SPE Solid phase extraction ST Simmental twins T Thymine Tab. Table TCM Traditional Chinese medicine TLC Thin layer chromatography tm Migration time U Uracil in case <strong>of</strong> DNA Unit <strong>of</strong> enzyme activity in case <strong>of</strong> description MN <strong>and</strong> SPD enzymes UV Ultraviolet UV-VIS Ultraviolet-visible V Volt V Voltage VECSEL Vertically external cavity surface emitting <strong>of</strong> laser 5
Page 1 and 2: The Influe
Page 3 and 4: The work at h<stro
Page 5 and 6: First, I feel always grateful to Al
Page 7: I would like to extend my thanks to
Page 11 and 12: The following acro
Page 13: FDA Food and drug
Page 18 and 19: Index 3.1 Spectrophotometeric deter
Page 20 and 21: Index 5.2.4.2 Dialysis of</
Page 22 and 23: Introduction 1.1.2 DNA methylation
Page 24 and 25: Introduction 1.2 The</stron
Page 26 and 27: Introduction Figure 4: Model for me
Page 28 and 29: Introduction Cancer is classified i
Page 30 and 31: Introduction DNA methylation Gene h
Page 32 and 33: Introduction 1.2.7.1 Clonin
Page 34 and 35: Introduction 1.2.8 Twins an
Page 36 and 37: Introduction chemicals, alkylating
Page 38 and 39: Introduction AP sites are not only
Page 40 and 41: Introduction 1.3.2.1 Oxidat
Page 42 and 43: Introduction Finally, flavo- <stron
Page 44 and 45: Introduction chromatography with el
Page 46 and 47: Introduction One of</strong
Page 48 and 49: Introduction facturing of</
Page 50 and 51: Introduction 320) ; but due to its
Page 52 and 53: Introduction HO O O Figure 15: Mech
Page 54 and 55: Introduction - Methylation Sensitiv
Page 56 and 57: Introduction 5‘ 5‘ 5 Figure 18:
Page 58 and 59: Introduction - Radioactive-mediated
Page 60 and 61: Aim and objectives
Page 62 and 63: Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Results and Discus
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Summary 4 Summary DNA methylation i
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Summary The same s
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Experimental part Sodium tetrapheny
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Experimental part � Sodium tetrab
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Experimental part framework was fas
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Experimental part propanol
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Experimental part 12.5 mU/µL SPD)
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Experimental part Condition Value D
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Experimental part 5.4 Instrumentati
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Experimental part Graduated cylinde
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Experimental part 3-Nitrobenzanthro
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Appendix In-vitro: Denoting a react
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Appendix Laboratory Document Hydrol
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Appendix BODIPY and</strong
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Appendix Description of</st
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Appendix Transmission [%] 120 100 8
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Appendix Figure 62: Mass spectrum E
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List of figures Fi
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List of tables 8 L
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Literature Literature 1. Egger, G.;
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Literature 41. Bestor, T.H.; "<stro
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Literature 78. Venitt, S.; "Mechani
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Literature 113. Sinclair, K.D.; Mce
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Literature 148. Kadan-Lottick, N.S.
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Literature cyte DNA by gas chromato
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Literature 221. Mirand</str
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Literature 257. Lingaraju, G.M.; Sa
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Literature different tissues <stron
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Literature 324. Vishwanath, B.S.; F
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Literature 359. Wei, L.; Li, R.K.;
Page 196 and 197:
Literature 394. Ramsahoye, B.H.; "M
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Literature 431. Wirtz, M.; Schumann
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Literature 467. Cornelius, M.; Wort
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Literature 502. Issa, J.P.J.; Ottav
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Literature 536. Pinheiro, J. <stron
Page 206:
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