Thin-Layer Chromatography

3.1.8 Miscellaneous Prechromatographic Derivatization 75
3.2 Postchromatographic Detection 77
3.2.1 Spraying 79
3.2.2 Dipping 82
3.2.3 Exposure to Vapors 86
3.2.4 Reagent in Solvent 88
3.2.5 Stationary Phase as Reagent (Reagent in Adsorbent) 88
3.2.6 Sequences of Spraying or Dipping 90
3.2.7 Processing the Chromatogram 90
3.2.7.1 Drying the Chromatogram 91
3.2.7.2 Effect of Heating after Application of Reagent 92
3.2.7.3 Stabilization of Developed Zones 98
3.3 Biological-Physiological Methods of Detection 109
4 Documentation and Hints for Chromatography Experts 119
4.1 Preparations for Chromatography 119
4.1.1 Solvent Quality 119
4.1.2 Choice of Stationary Phase 121
4.1.3 Prewashing the Layer 124
4.1.4 Choice of Chamber System 124
4.2 Documentation on the Chromatogram 131
4.3 Fixing the Visual Appearance of the Chromatogram 133
4.3.1 Preserving with Neatan 134
4.3.2 Documentation by Sketching, Photocopying or Photographing 134
4.4 Documentation by Means of in situ Evaluation by Computer. . 138
Partn
Reagents in Alphabetical Order
Alizarin Reagent 143
Aluminium Chloride Reagent 147
4-Aminoantipyrine - Potassium Hexacyanoferrate(III) Reagent 151
4-Aminobenzoic Acid Reagent 154
4-Aminodiphenyl — Sulfuric Acid Reagent 157
4-Aminohippuric Acid Reagent 160
4-Aminohippuric Acid — Phthalic Acid Reagent 163
Ammonia Vapor Reagent 166
Ammonium Thiocyanate — Iron(III) Chloride Reagent 170
Amylose - Potassium Iodate/Iodide Reagent 173
Aniline — Aldose Reagent 176
Aniline - Diphenytamine - Phosphoric Acid Reagent 179
Aniline - Phosphoric Acid Reagent 185
Aniline - Phthalic Add Reagent 188
8-Anilinonaphthalene-l-sulfonic Acid Ammonium Salt Reagent 191
Anisaldehyde — Sulfuric Acid Reagent 195
p-Anisidine - Phthalic Acid Reagent 199
Anthrone Reagent 202
Antimony(HI) Chloride Reagent 206
AntimonyOO Chloride Reagent 210
Berberine Reagent 213
2,2'-Bipyridine - Iron(III) Chloride Reagent 216
Blue Tetrazolium Reagent 219
Bratton-Marshall Reagent 223
Bromocresol Green — Bromophenol Blue —
Potassium Permanganate Reagent 228
Bromocresol Purple Reagent 231
tert-Butyl Hypochlorite Reagent 234
7-Chloro-4-nitrobenzo-2-oxa-l,3-diazole Reagent 238
Copper(II) Acetate — Phosphoric Acid Reagent 242
Copper(II) Nitrate Reagent 245
Copper(II) Sulfate Reagent 248
2,6-Dibromoquinone-4-chloroimide Reagent 252
2,6-Dichlorophenolindophenol Reagent 256
2,6-Dichloroquinone-4-chloroimide Reagent 260
2,5-Dimethoxytetrahydrofuran — 4-Dimethylaminobenzaldehyde Reagent 265
4-Dimethylamino-cinnamaldehyde — Hydrochloric Acid Reagent .... 269
2,4-Dinitrophenylhydrazine Reagent 273
Diphenylboric Acid 2-Aminoethyl Ester Reagent 277
Diphenylboric Anhydride Reagent 281
Diphenylboric Anhydride — Salicylaldehyde Reagent 284
Fast Blue Salt B Reagent 288
Fluorescamine Reagent 294
Formaldehyde — Sulfuric Acid Reagent 299
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