Thin-Layer Chromatography

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Parti Methods of Detection
1 Introduction The separation methods routinely employed in the laboratory include the various chromatographic and electrophoretic techniques, whose selectivity is continually being increased by the introduction of new adsorbents, e.g. with chemically modified surfaces (Fig. 1). Detection methods, which provide real information concerning the separated substances, are necessary in order to be able to analyze the separation result and separation performance achieved by such a system. Cn,ral layers Future developments Fig. 1: Schematic view of the surface-modified silica gels at present commercially available.
Page 1 and 2: Hellmut Jork, Werner Funk, Walter F
Page 3 and 4: VI Foreword in many cases, an elega
Page 5 and 6: Contents Parti Methods of Detection
Page 7: A1V L,omenis Hydrochloric Acid Vapo
Page 11 and 12: Number 600 too 200 1 Introduction 1
Page 13 and 14: Colorless substances absorb at wave
Page 15 and 16: 14 rnysicat Methods oj Detection B
Page 17 and 18: io z rnysicai Meinous oj ueieciwn F
Page 19 and 20: 22 2 Physical Methods of Detection
Page 23 and 24: 3U z rnysicai meinous oj veiecuun P
Page 35 and 36: 3 Chemical Methods of Detection Eve
Page 37 and 38: Chemical Meinoas oj ueiecuun Fig. 3
Page 39 and 40: 62 3 Chemical Methods of Detection
Page 47 and 48: uj • improving the detection sens
Page 49 and 50: 82 3 Chemical Methods oj Detection
Page 59 and 60:
30-- 20 4- 10 •• P-7 P-8 —I 1
Page 61 and 62:
106 3 Chemical Methods of Detection
Page 63 and 64:
Page 65 and 66:
lit J ^ncniiLui lvicinuus uj [129]
Page 67 and 68:
Page 69 and 70:
122 4 Documentation and Hints for C
Page 71 and 72:
T LsvLumcniuuuri unu liiruijur \^nr
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Page 75 and 76:
zone size and distribution of subst
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Page 79 and 80:
Reagent for: Cations [1-7] Preparat
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146 Alizarin Reagent Detection and
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150 Aluminium Chloride Reagent UV l
Page 85 and 86:
4-Aminobenzoic Acid Reagent Reagent
Page 87 and 88:
158 2-Aminodiphenyl — Sulfuric Ac
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162 4-Aminohippuric Acid Reagent 1
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Reagent for: Ammonia Vapor Reagent
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Ammonium Thiocyanate — Iron(III)
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174 Amy lose — Potassium lodate/I
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1/0 Anuine — Aiaose neageni Mobil
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15 L Anmne — uipnenyiamine — rn
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186 Aniline — Phosphoric Acid Rea
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i s\j Jiituuuz — -I ninuiH. S1LIU
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li»4 X-Anilinonaphthalene-1-sulfon
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198 Anisaldehyde — Sulfuric Acid
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Reagent for: • Ketoses [1] • Gl
Page 111 and 112:
Antimony(III) Chloride Reagent (Car
Page 113 and 114:
Antimony(V) Chloride Reagent Reagen
Page 115 and 116:
214 Berberine Reagent Under UV ligh
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218 2,2'-Bipyridine - Iron(III) Chl
Page 119 and 120:
222 Blue Tetrazolium Reagent Note:
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224 Bratton-Marshall Reagent Substa
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Bromocresol Green — Bromophenol B
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232 Bromocresol Purple Reagent Meth
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236 tert-Butyl Hypochlorite Reagent
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240 7-Chloro-4-nitrobenzo-2-oxa-l,3
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244 Copper (II) acetate — Phospho
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Copper(II) Sulfate Reagent Reagent
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2,6-Dibromoquinone-4-chloroimide Re
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2,6-Dichlorophenolindophenol Reagen
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296-Dichloroquinone-4-chloroimide R
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204 Zp-Uichloroqumone-4-cMorovniOe
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268 2,5-Dimethoxytetrahydrofuran
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Lii. t-uimeinyiummocmnumaiaenyae
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276 ^,4-Uimtrophenylhydrazine Reage
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280 Diphenylboric acid-2-aminoethyl
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Diphenylboric Anhydride — Salicyl
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Reagent for: Fast Blue Salt B Reage
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tasi mue salt a Keagent 1 5 10 15 2
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ZVb tluorescamine Keagent Sulfonami
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300 Formaldehyde - Sulfuric Acid Re
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304 Hydrochloric Acid Vapor Reagent
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308 Hydrogen Peroxide Reagent radia
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312 8-Hydroxyquinoline Reagent Proc
Page 167 and 168:
310 iron(iii) L^moriae — fercnion
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5Z\) isonicotinic Acid Hydrazide Re
Page 171 and 172:
JZt Ljeaa{ii) Aceiaie Basic neagem
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In situ quantitation: The fluorimet
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332 Lead(IV) Acetate — Fuchsin Re
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JJO manganeseinj ^nionae — zuijun
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Mercury(II) Salt - Diphenylcarbazon
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2-Methoxy-2,4-diphenyl-3(2H) furano
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jis 3-Meinyi-z-Denzotniazoiinone-ti
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jji i,*-ivupninoquinone-4-sulJonic
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JDO mnnyarui — ^.oiuatne iteagent
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360 4-(4-Nitrobenzyl)pyridine Reage
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Reagent for: • Steroids [1-8] •
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Peroxide Reagent (1-Naphthol - N 4
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1,2-Phenylenediamine — Trichloroa
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Phosphomolybdic Acid Reagent Reagen
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Reagent for: o-Phthalaldehyde Reage
Page 201 and 202:
384 o-fhthaiaiaenyae neageni [16] G
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Pinacryptol Yellow Reagent Reagent
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Potassium Hexacyanoferrate(III) —
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396 Potassium Hexacyanoferrate(III)
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4UU fyrocatechol Violet Reagent 1 2
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Reagent for: Rhodamine 6G Reagent
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Silver Nitrate — Sodium Hydroxide
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Preparation of Reagent Dipping solu
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Tetracyanoethylene Reagent (TCNE Re
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Trichloroacetic Acid Reagent Reagen
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tz.t i rinuruoenzenesuijonw ACIU ne
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4/5 vanadium{ v) — auijunc Acid R
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432 Vanillin — Phosphoric Acid Re
Page 227 and 228:
t JO vamuin — roiassium tiyaroxid
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Layer Mobile phase Migration distan
Page 231 and 232:
Index ABP = 2-amino-5-bromophenyl(p
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Benztriazole -, derivatives 281 ff
Page 235 and 236:
Disaccharides 154,161,163,179,181,
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430 maex Isoniazide 318 Isonicotini
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40U maex -, side on 27 -, window ma
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464 Index Triphenodioxazines 411 Tr
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Prof. Dr. H. Jork UniversitSt des S
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VIII Preface to Volume lb Particula
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Contents Foreword Preface to Volume
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XVI Contents Potassium Dichromate -
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4 Introduction For the same reason
Page 253 and 254:
8 Introduction [36] Wilson, I. D.,
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12 / Activation Reactions The inorg
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16 1 Activation Reactions The excit
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20 1 Activation Reactions These few
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24 1 Activation Reactions Migration
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28 / Activation Reactions of malona
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32 / Activation Reactions Table 2.2
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36 / Activation Reactions In situ q
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40 / Activation Reactions [4] Egg,
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2 Reagents for the Recognition of F
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48 Table 4: (continued) Functional
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52 2 Reagents for the Recognition o
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3 Reagent Sequences Thin-layer chro
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60 5 Reagent Sequences A B Fig. 23:
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t>4 5 Reagent Sequences Here the al
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68 3 Reagent Sequences tert-Butyl H
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72 3 Reagent Sequences tert-Bnty\ H
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76 3 Reagent Sequences Cerium(IV) S
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80 3 Reagent Sequences Sodium Hydro
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84 3 Reagent Sequences Sodium Hydro
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OO J xvcugcric uc Tin(II) Chloride-
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92 3 Reagent Sequences Tkanium(III)
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96 3 Reagent Sequences Reagent 4 Co
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100 3 Reagent Sequences Nitric Acid
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104 3 Reagent Sequences Reagent 2 R
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108 3 Reagent Sequences Tin(Il) Chl
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112 3 Reagent Sequences 1 2 3 4 M 5
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116 3 Reagent Sequences air. It was
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120 3 Reagent Sequences Hydroxylami
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124 3 Reagent Sequences Borate Buff
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128 3 Reagent Sequences Diphenylami
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132 3 Reagent Sequences Tible 1: Co
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136 3 Reagent Sequences Reaction Th
Page 319 and 320:
140 3 Reagent Sequences [36] Bomtra
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144 Acridine Orange Reagent Reactio
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148 Ammonium Monovanadate-p-Anisidi
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152 Ammonium Thiocyanate Reagent Me
Page 327 and 328:
156 4,4'-Bis(dimethylamino)thiobenz
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160 N-Bromosuccinimide Reagent Proc
Page 331 and 332:
164 N-Bromosuccinimide-Robinetin Re
Page 333 and 334:
168 Cacotheline Reagent Method The
Page 335 and 336:
172 Chloramine T Reagents Table 1:
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176 Chloramine T-Mineral Acid Reage
Page 339 and 340:
180 Chloramine T-Sodium Hydroxide R
Page 341 and 342:
184 Chloramine T- Trichloroacetic A
Page 343 and 344:
Reagent for: /7-Chloranil Reagent
Page 345 and 346:
192 p-Chloranil Reagent Procedure T
Page 347 and 348:
196 Chlorine-Potassium Iodide-Starc
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200 Chlorine-4,4'-Tetramethyldiamin
Page 351 and 352:
Reagent for: Chlorine - o-Tolidine
Page 353 and 354:
208 Chlorine-o-Tolidine-Potassium I
Page 355 and 356:
Cyanazin (hR{ 5-10) appeared as gra
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216 Copper(II) Sulfate-Sodium Citra
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220 Dansyl Chloride Reagent Reactio
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224 Dimedone-Phosphoric Acid Reagen
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228 N,N-Dimethyl-l,4-phenylenediami
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4-(Dimethylamino)benzaldehyde - Ace
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4-(Dimethylamino)benzaldehyde-Acid
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240 4-(Uimethyiammo)-oenzaiaenyae-A
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244 4-(Dimethylamino)-benzaldehyde-
Page 373 and 374:
Page 375 and 376:
4-(Dimet hy lamino)-benzaldehyde -
Page 377 and 378:
Page 379 and 380:
260 Dimethylglyoxime Reagent Reacti
Page 381 and 382:
264 3,5-Dinitrobenzoic Acid-Potassi
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Fast Black Salt K- Sodium Hydroxide
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272 Fast Black Salt K-Sodium Hydrox
Page 387 and 388:
276 Fast Blue Salt BB Reagent Refer
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280 Iodine Reagents Table 1: (conti
Page 391 and 392:
284 Iodine Vapor Reagent Reaction I
Page 393 and 394:
288 Iodine Vapor Reagent [4] Andree
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292 Iodine Solution, Neutral Reagen
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Iodine-Potassium Iodide Solution, A
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300 Iodine-Potassium Iodide Solutio
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304 Iodine-Potassium Iodide Solutio
Page 403 and 404:
3U8 lron(iii) cnionae Reagent Prepa
Page 405 and 406:
Iron(III) Chloride-Potassium Hexacy
Page 407 and 408:
316 Iron(III) Chloride-Potassium He
Page 409 and 410:
320 8-Mercaptoquinoline Reagent Ref
Page 411 and 412:
324 l,2-Naphthoquinone-4-sulfonic A
Page 413 and 414:
328 Nitric Acid Vapor Reagent Prepa
Page 415 and 416:
Reagent for: 4-Nitrobenzenediazoniu
Page 417 and 418:
References [1] Fuhremann, T. W., Li
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340 Palladiumfll) Chloride Reagent
Page 421 and 422:
344 Phosphoric Acid Reagent Storage
Page 423 and 424:
Reagent for: 0-Phthalaldehyde- Sulf
Page 425 and 426:
Potassium Dichromate-Perchloric Aci
Page 427 and 428:
1 3 5 6 7 8 9 10 11 12 Fig 2: Chrom
Page 429 and 430:
Reaction The mechanism of the react
Page 431 and 432:
364 Potassium Hexaiodoplatinate Rea
Page 433 and 434:
joo tvtassium Hydroxide Reagent Lay
Page 435 and 436:
Preparation of the Reagent Dipping
Page 437 and 438:
Potassium Nitrate-Sulfuric Acid Rea
Page 439 and 440:
380 Potassium Peroxodisulfate-Silve
Page 441 and 442:
Reaction The reaction mechanism has
Page 443 and 444:
388 Sodium Hydroxide Reagent Prepar
Page 445 and 446:
References ouuium tiyaroxiae Reagen
Page 447 and 448:
In situ quantitation: The absorptio
Page 449 and 450:
c o Fig. I: Reflectance scans of a
Page 451 and 452:
Procedure Tested Nitrophenols [28]
Page 453 and 454:
408 Sulfanilic Acid-N-(l-Naphthyl)-
Page 455 and 456:
412 Tetrabromophenolphthalein Ethyl
Page 457 and 458:
,j\-ietramethyl-l,4-phenylenediamin
Page 459 and 460:
4-nitroaniline) and 25 ng (2-amino-
Page 461 and 462:
424 Thymol-Sulfuric Acid Reagent Fi
Page 463 and 464:
4/8 i m[ii) ^nionae-nyarocnionc Aci
Page 465 and 466:
432 Tin(IV) Chloride Reagent Migrat
Page 467 and 468:
436 Titaniumflll) Chloride-Hydrochl
Page 469 and 470:
Vanillin Reagents The "aldehyde aci
Page 471 and 472:
Procedure Tested Indole Derivatives
Page 473 and 474:
IVICUIUU The chromatograms are drie
Page 475 and 476:
452 Vanillin-Sulfuric Acid Reagent
Page 477 and 478:
456 Named Reagents and Reagent Acro
Page 479 and 480:
460 Index Aminoglycoside antibiotic
Page 481 and 482:
464 Index Cactus alkaloids lb 229 C
Page 483 and 484:
468 Index -, phenols la 73 -, prech
Page 485 and 486:
472 Index Ergocornine lb 243 Ergocr
Page 487 and 488:
476 Index Halogen lamp la 22 Hexoba
Page 489 and 490:
480 Index Lipids la 44-46, 89,191,2
Page 491 and 492:
484 Index Orelline lb 307 Orellinin
Page 493 and 494:
488 Index PRimine lb 244,246,247 Pr
Page 495 and 496:
492 Index Stimulents lb 268,270 Str
Page 497:
496 Index -, bioautoradiographic de
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Thin-Layer Chromatography

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