XXIIIrd International Eucarpia symposium, Section Ornamentals ...

POTENTIAL COMMERCIAL VALUE FROM
MUTATED CLONES OF CALADIUM
HUMBOLDTII SCHOTT ‘PHRAYA SAVET’
FROM IN VITRO CULTURE
Mutated clones of Phraya Savet caladium (Caladium humboldtii cv. ‘Phraya Savet’) from
in vitro culture were observed. Callus and small shoots were induced from unexpanded
leaf segments cultured on modified Murashige and Skoog medium (MS) supplemented
with 2.69 µM 1-Naphthalene acetic acid (NAA) and 17.76 µM N 6 -Benzyladenine (BA) for
4 – 5 months with subculturing every 6 weeks. Shoots were transferred onto modified
MS medium supplemented with 8.88 µM BA for shoot multiplication. Subsequently, roots
were induced on MS without growth regulator for 2 weeks. The regenerated plantlets
were vigorously grown in glasshouse conditions. From 3 morphological groups, leaf
pattern, petiole and leaf colour were used to identify the mutated clones. The
regenerated ‘Phraya Savet’ caladium plants were divided into 11 types. Variations were
found in 10 types with multiple variants from 1 – 4 characters. The occurrence of variants
was 34 percent. Leaf pattern variants were observed at the highest frequency of 28
percent while leaf colour variants and petiole variants were found 16.0 and 4%,
respectively. The most significance for commercial value from mutated clones was round
leaf (9 percent). Other interesting variations were discussed.
P68
K. Obsuwan
C. Thepsithar
A. Thongpukdee
U. Somkanea
Department of Biology,
Faculty of Science,
Silpakorn University,
Sanamchandra Palace
Campus, Muang, Nakhon
Pathom 73000, Thailand
kulanart@su.ac.th
Session Posters 141