XXIIIrd International Eucarpia symposium, Section Ornamentals ...
XXIIIrd International Eucarpia symposium, Section Ornamentals ...
XXIIIrd International Eucarpia symposium, Section Ornamentals ...
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LP10<br />
W. Tera-arusiri (1)<br />
S. Mahadtanapuk (1)<br />
S. Anuntalabhochai (2)<br />
M. Sanguansermsri (3)<br />
W. Nanakorn (3)<br />
(1) School of Agriculture<br />
and Natural Resources,<br />
Naresuan University<br />
Phayao, Tumbol Maeka,<br />
Muang, Phayao, 56000,<br />
Thailand<br />
(2) Department of Biology,<br />
Faculty of Science, Chiang<br />
Mai University, Chiang Mai<br />
50200, Thailand<br />
(3) Naresuan University<br />
Phayao, Tumbol Maeka,<br />
Muang, Phayao, 56000,<br />
Thailand<br />
werachai_kig_bu@<br />
hotmail.com<br />
BREEDING FOR RESISTANCE AND<br />
BIOCONTROL OF WILT DISEASE IN<br />
CURCUMA ALISMATIFOLIA GAGNEP BY<br />
BACILLUS SPP.<br />
The objectives of this study were to breed for resistance of Curcuma alismatifolia<br />
Gagnep to wilt disease caused by Rastonia species, by screening and collecting virulent<br />
isolates of Rastonia Moreover, in order to preserve the value of these ornamental<br />
flowers, and to reduce the dependence on toxic chemicals required to combat this<br />
disease the biocontrol potential of bacteria antagonistic to Rastonia, , was investigated.<br />
Over 500 bacterial strains, isolated from soil, leaf surfaces of C. alismatifolia Gagnep.<br />
and hot springs in the Chiang Mai province in Thailand, were screened in vitro for<br />
antagonistic activity against R. solanacerarum. Three isolates providing growth inhibition<br />
in vitro , were identified as Bacillus licheniformis, B.amyloliquefaciens and B. subtilis.<br />
Subsequently, these isolates were used for biocontrol in planta. One of the isolates, B.<br />
subtilis was shown inhibition greater than 70% when compared with the control and<br />
provided a statistically significant growth suppression of the wilt disease on the curcuma.<br />
Moreover, all of the antagonistic bacteria isolates were selected to against Anthracnose<br />
caused by Colletotrichum sp. in planta. The highest levels of the Anthracnose disease<br />
suppression occurred using B. licheniformis and B. subtilis on curcuma flower. The<br />
present results indicate that B. licheniformis, B.amyloliquefaciens and B. subtilis could be<br />
used to inoculate and reduce the symptoms of disease on Curcuma alismatifolia<br />
Gagnep.<br />
40 Session Short presentations
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