XXIIIrd International Eucarpia symposium, Section Ornamentals ...

LP16
T. Basaki (1)
M. Jafarkhani Kermani
(1)
S.M. Pirseyedi (1)
M.R. Ghaffari (1)
A. Haghnazari (2)
P. Salehi Shanjani (3)
P. Koobaz (1)
M. Mardi (1)
(1) Agricultural
Biotechnology Research
Institute of Iran (ABRII),
Mahdasht Road, Karaj, Iran
(2) Zanjan University,
Zanjan, Iran
(3) Research Institute of
Forests and Rangelands,
P.O.Box 13185-116,
Tehran, Iran
mardi@abrii.ac.ir
ASSESSING ROSA PERSICA GENETIC
DIVERSITY USING AMPLIFIED FRAGMENT
LENGTH POLYMORPHISMS ANALYSIS
The genetic diversity among 128 Iranian Rosa persica (R. persica) accessions in the
different populations was analyzed. Amplified fragment length polymorphisms (AFLP)
technique was used to produce 171 polymorphic fragments. The number of polymorphic
loci ranged from 101 to 147 and the polymorphism information content (PIC) varied from
0.289 to 0.073, with an average of 0.16. This shows extreme variability and genetic
diversity among the studied R. persica populations. An indirect estimate of the number of
migrants per generation (Nm=0.376) indicated that gene flow was relatively low among
populations of the species. Cluster analysis using the UPGMA method grouped all
accessions into six clusters. The results did not show relative agreement with the
genotypes’ region of origin. Based on an analysis of molecular variance, 48% of the
genetic variation of R. persica was within population and 52% was among populations.
The present analysis revealed that Iranian R. persica genotypes are highly variable and
genetically distinct from their origins. The apparent unique nature of the R. persica
genotypes revealed by our results supports the case for the implementation of more
intense characterization and conservation strategies, and provides useful information to
address breeding programmes and germplasm resource management in Rosa spp.
60 Session Short presentations