(ScAg 1501) Final Exam—May 8, 2002 Multiple Choice. Cho

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20. Single nucleotide polymrphisms can: a. Be used as markers associated with a trait b. Are polymorphisms that are present in a species about every 1000 bp c. Be used to predict the 3-D conformation of a protein d. Both a and b e. Both b and c 21. Dolly the cloned Finn Dorset was the first animal derived a. Using a Nucleus from an adult cell b. Using mitochondria c. Using tissue DNA d. Using embryonic cells 22. These cells don’t stop growing when they come in contact with each other: a. Primary b. Tumor c. Old d. Immortal, but not tumorigenic e. Normal 23. A DNA chip: a. Represents thousands of expressed sequence tags b. Can serve as probes for determining expression levels of many different mRNAs c. Both a and b d. Is used for the expression analysis of only one gene at a time 24. One of the following answers is NOT true for short tandem repeats: a. They can identify 1 in 10 18 individuals (when all 13 STRs are analyzed) b. They are based on highly polymorphic regions of DNA among individuals c. They have replaced RFLP analysis in most state crime labs d. They use Southern blot technology 25. The CFTR gene was identified by all of the following methods except: a. Purification of the mutant protein b. Chromosome walking c. Chromosome Mapping d. DNA sequencing e. Genetic linkage analyis 26. Treatment for CFTR currently uses gene therapy where the adenovirus: a. Homes to the lungs b. Is replication defective c. Homes to the liver d. Both a and b
Fill in the blank (2 pts./question;14 pts. total). __________1. Name a product produced by biopharming. __________2. One of the desired traits that might be acquired through the production of transgenic farm animals. __________3. Modifications to proteins that are often times necessary for complete biological and functional activity. __________4. Transgenic fish that express this protein can live in freezing waters off Newfoundland. __________5. The type of gene therapy whereby any changes in the genome are NOT passed on the next generation. __________6. These genes (or gene products) suppre controlled regulated expression of a potential cancer causing gene. __________7. To be successful, gene therapy needs to produce proteins that are toxic at high levels (T or F).
Page 1 and 2: Name______________________ Biotechn
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(ScAg 1501) Final Exam—May 8, 2002 Multiple Choice. Cho

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