icvg 2009 part I pp 1-131.pdf - Cornell University

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— 26 — SEROLOGICAL RELATIONSHIPS AMONG GLRAVS The study of serological relationships between GLRaVs by means of antiserum is ambiguous since immunogens from grapevine used for their production may not have been homogenous. Some information can however be gained with Mabs. Seddas et al., (2000) reported about Mabs made against GLRaV-1 that cross-reacted with GLRaV-3. A recAb homologous to GLRaV-3 also crossreacted with GLRaV-1, 3, 6 and 7 but not with GLRaV-2 (Orecchia et al., 2008). Others made against GLRaV-8 cross-reacted with either GLRaV-5 (Mab 15F1) or GLRaV- 4 and GLRaV-5 (Mab 3F76) (Monis et al., 2000). Significant cross-reactions are indeed observed within the group of GLRaV-4, 5, 6 and 9 with several Mabs made recently at Agroscope Nyon (Table 2), proving the closer relationship of species within this subgroup of Ampelovirus, as recently defined by molecular studies (Alkowni et al., 2003, 2004, Maliogka et al., 2009). Table 2. Serological distinction of GLRaVs by ELISA using Mabs developed at Agroscope ACW Nyon, Switzerland. CONCLUSIONS Antibodies have played a key role for the identification of GLRaVs since 25 years. Monoclonal antibodies were particularly precise biological tools. They will remain convenient for virus detection when sampling and sample processing are correctly done. RecAb may complete them in the future and render serology still attractive, although slightly less sensitive than some molecular techniques. A four to five-digit number of grapevine samples tested worldwide by serology could very likely be used to ascertain statistically more tightly the causal relationship between GLRaVs and grapevine leafroll disease. LITERATURE ALKOWNI, R. & ROWHANI, A. 2003. Molecular characterization of Grapevine leafroll-associated virus 9, a new closterovirus associated with grapevine leafroll disease complex. In: Extended abstracts 14th Meeting of the ICVG, Locorotondo, Italy, 33. ALKOWNI, R., ROWHANI, A., DAUBERT, S. & GOLINO, D. 2004. Partial characterization of a new ampelovirus associated with grapevine leafroll disease. Journal of Plant Pathology 86, 123-133.. BESSE, S., BALMELLI, C., HOFSTETTER, V. & GUGERLI, P. 2009a. Serological and molecular characterisation of Grapevine leafroll associated virus 2 variants occurring in Switzerland. These proceedings. BESSE S., BITTERLIN, W. & GUGERLI, P. 2009b. Development of an ELISA for the simultaneous detection of the Grapevine leafrollassociated viruses 4, -5, -6, -7 and -9. These proceedings. BOSCIA, D., GREIF, C., GUGERLI, P., MARTELLI G. P., WALTER, B. & GONSALVES, D. 1995. Nomenclature of grapevine leafroll-associated putative closteroviruses. Vitis 34, 171- 175. BOSCIA, D., DIGIARO, M., SAVINO, V., & MARTELLI, G. P. 2000. Grapevine leafroll-associated virus 6 and Vitis vinifera cv. Cardinal: an intriguing association. In: Extended abstracts 13th Meeting of the ICVG, Adelaide, Australia, 21-22. CASTELLANO, M.A., MARTELLI, G.P., & SAVINO, V. 1983. Virus-Like Particles and Ultrastructural Modifications in the Phloem of Leafroll-Affected Grapevines. Vitis 22 (1), 23-39. CHOUEIRI, E., BOSCIA, D., DIGIARO, M., CASTELLANO, M.A. & MARTELLI, G.P. 1996. Some properties of a hitherto undescribed filamentous virus of the grapevine. Vitis 35:91-93. COGOTZI, L., GIAMPETRUZZI, A., NOLKE, G., ORECCHIA, M., ELICIO, V., CASTELLANO, M.A., MARTELLI, G.P., FISCHER, R., SCHILLBERG, S. & SALDARELLI, P. 2009. An assay for the detection of grapevine leafroll-associated virus 3 using a single-chain fragment variable antibody. Archives of Virology 154 (1), 19-26. CONTI, M., MILNE, R.G., LUISONI, E. & BOCCARDO, G. 1980. A closterovirus from a stem-pitting-diseased grapevine. Phytopathology 70, 394-399. FAGGIOLI, F., PASQUINI, G., RICCIONI, L. & BARBA, M. 1991. Further characterization and serology of grape leafroll associated virus III isolated from grape in Italy. Extended abstracts 10th Meeting of ICVG, Volos, Greece, 473-476. FAORO F.,. TORNAGHI R, FORTUSINI A. AND BELLI G. 1981. Association of a possible closterovirus with grapevine leafroll in northern Italy. Riv.Patol.Veg.,Ser.IV 17, 183-189. ENGEL, E.A., GIRARDI, C., ESCOBAR, P.F., ARREDONDO, V., DOMINGUEZ, C., PEREZ-ACLE, T. & VALENZUELA, P.D.T. 2008. Genome analysis and detection of a Chilean isolate of Grapevine leafroll associated virus-3. Virus Genes 37 (1), 110-118.
— 27 — ENGELBRECHT, D.J. & KASDORF, G.G.F. 1990. Transmission of grapevine leafroll disease and associated closteroviruses by the vine mealybug, Planococcus ficus. Phytophylactia 22, 341-345. GOSZCZYNSKI, D.E., KASDORF, G.G.F. & PIETERSEN, G. 1995. Production and use of antisera specific to grapevine leafrollassociated viruses following electrophoretic separation of their proteins and transfer to nitrocellulose. Afr.Pl.Prot. 1(1), 1-8. GOSZCZYNSKI, D.E., KASDORF, G.G.F., PIETERSEN G., & VAN TONDER, H. 1996a. Grapevine leafroll-associated virus 2 (GLRaV-2) - Mechanical transmission, purification, production and properties of antisera, detection by ELISA. South African Journal of Enology and Viticulture 17, 15-26. GOSZCZYNSKI, D.E., KASDORF, G.G.F., PIETERSEN, G. & VAN TONDER, H. 1996b. Detection of two strains of grapevine leafrollassociated virus 2. Vitis 35, 133-135. GUGERLI, P., BRUGGER, J.J. & BOVEY, R. 1984. L'enroulement de la vigne: mise en évidence de particules virales et développement d'une méthode immuno-enzymatique pour le diagnostic rapide (Grapevine leafroll: presence of virus particles and development of an immuno-enzyme method for diagnosis and detection). Revue Suisse de Viticulture, Arboriculture et Horticulture 16, 299-304. GUGERLI, P. 1987. Gra,pevine leafroll disease: rapid diagnosis by electron microscopy and serology. Schw.landw.Forschung/La Recherche agronomique en Suisse 26, 388-389. GUGERLI P., BRUGGER, J.J. & BASLER, P. 1990. Les maladies de l'enroulement, du bois strié et de l'écorce liégeuse de la vigne (Grapevine leafroll, rugose wood and corky bark). Revue Suisse de Viticulture, Arboriculture et Horticulture 22, 35-36. GUGERLI, P., ROSCIGLIONE, B., BRUGGER, J.J., BONNARD, S., RAMEL, M.E. & TREMEA, F. 1990. Etiological studies and diagnostic of grapevine leafroll disease improved by Mabs. In: Monoclonal antibodies in agriculture. Proceedings Symposium "Perspectives for monoclonal antibodies in agriculture", Wageningen, Netherlands, May 1990, edited by A. Schots, Wageningen:Pudoc, 1990, 47-54. GUGERLI, P. & RAMEL M.E. 1993. Grapevine leafroll associated virus II analyzed by monoclonal antibodies. Extended abstracts 11th Meeting of ICVG, Montreux, Switzerland, 23-24. GUGERLI, P., BRUGGER, J.J. & RAMEL, M.E. 1997. Identification immuno-chimique du 6e virus associé à la maladie de l'enroulement de la vigne et amélioration des techniques de diagnostic pour la sélection sanitaire en viticulture. Revue Suisse de Viticulture, Arboriculture et Horticulture 29, 137-141. GUGERLI P., RIGOTTI, S., RAMEL, M.E., HABILI, N., ROWHANI, A. & BESSE, S. 2009. Development of monoclonal antibodies and the serological identification of GLRaV-9. These proceedings. HU, J.S., BOSCIA, D. & GONSALVES, D. 1989. Use of monoclonal antibodies in the study of closteroviruses associated with grape leafroll disease. Phytopathology 79, 1189. HU, J. S., GONSALVES, D., BOSCIA, D., & NAMBA, S. 1990a. Use of monoclonal antibodies to characterize grapevine leafroll associated closteroviruses. Phytopathology 80, 920-925. HU, J.S., GONSALVES, D. & TELIZ, D. 1990b. Characterization of closterovirus-like particles associated with grapevine leafroll disease. Journal of Phytopathology 128, 1-14. LEGIN, R., LE GALL, O., ZIMMERMANN, D., BASS, P., WALTER, B., MEIGNOZ, R. & CAUDWELL, A. 1989. Closterovirus-like particles in Chardonnay infected with the "vein yellowing leafroll" disease in Champagne, France. Proceedings 9th meeting of ICVG, Kiryat Anavim, Israel, 6-11 Sept 1987, 97-106. LING, K.S., ZHU, H.Y., JIANG, Z.Y., McFERSON, J.R. & GONSALVES, D. 1997. Application of ELISA for virus detection using a polyclonal antibody produced from a recombinant coat protein of grapevine leafroll virus 3 expressed in Escherichia coli. Extended abstracts 12th Meeting of ICVG, Lisbon, Portugal, 89. LING, K.S., ZHU, H.Y., JIANG, Z.Y., & GONSALVES, D. 2000. Effective application of DAS-ELISA for detection of grapevine leafroll associated closterovirus-3 using a polyclonal antiserum developed from recombinant coat protein. European Journal of Plant Pathology 106, 301-309. LING, K.S., ZHU, H.Y., PETROVIC, N. & GONSALVES, D. 2007. Serological detection of Grapevine leafroll virus 2 using an antiserum developed against the recombinant coat protein. Journal of Phytopathology 155 (2), 65-69. MADDEN E.G., ZEE, F. & GONSALVES, D. 1987. Production of monoclonal antibodies to closterovirus-like particles associated with grapevine leaf roll disease. Phytopathology 77, 120. MALIOGKA, V.I., DOVAS, C.I., LOTOS, L., EFTHIMIOU, K. & KATIS, N.I. 2009. Complete genome analysis and immunodetection of a member of a novel virus species belonging to the genus Ampelovirus. Archives of Virology 154 (2), 209-218. MARTELLI, G.P., AGRANOVSKI, A.A., BAR-JOSEPH, M., BOSCIA, D., CANDRESSE, T., COUTTS, R.H.A., DOLJA, V.V., FALK, B.W., GONSALVES, D., JELKMANN, W., KARASEV, A.V., MINAFRA, A., NAMBA, S., VETTEN, H.J., WISLER, G.C. & YOSHIKAWA, N. 2002. The family Closteroviridae revised. Archives of Virology 147, 2039-2044. NAMBA, S., YAMASHITA, S., DOI, Y., YORA, K., TERAI, Y. & YANO, R. 1979. Grapevine leafroll virus, a possible member of closteroviruses. Annals of the Phytopathology Society of Japan 45, 497-502. NAMBA, S., BOSCIA, D., AZZAM, O., MAIXNER, M., HU, J. S., GOLINO, D. & GONSALVES, D. 1991. Purification and properties of closteroviruslike particles associated with grapevine corky bark disease. Phytopathology 81, 964-970. MONIS, J. & BESTWICK, R.K. 1997. Production of monoclonal antibodies specific to grapevine associated closteroviruses. Extended abstracts 12th Meeting of ICVG, Lisbon, Portugal, 105. MONIS, J. & BESTWICK, R.K. 1997. Serological detection of grapevine associated closteroviruses in infected grapevine cultivars. Plant Disease 81, 802-808. MONIS, J. 2000. Development of monoclonal antibodies reactive to a new grapevine leafroll-associated closterovirus. Plant Disease 84, 858-862. NÖLKE, G., ORECCHIA, M., SALDARELLI P., DELL'ORCO, M., MINAFRA, A., MARTELLI G.P., FISCHER, R., & SCHILLBERG, S. 2003. Antibody-based resistance in grapevine: generation, characterization and expression of single chain antibody fragments specific to Grapevine leafroll-associated virus 3. Extended abstracts 14th Meeting of ICVG, Locorotondo, Italy, 232bis. ORECCHIA, M., NOELKE, G., SALDARELLI, P., DELL'ORCO, M., UHDE-HOLZEM, K., SACK, M., MARTELLI G.P, FISCHER, R. & SCHILLBERG, S. 2008. Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus 3. Archives of Virology 153 (6), 1075-1084. RADAELLI, P., FAJARDO, T.V.M., NICKEL, O., EIRAS, M. & PIO-RIBEIRO, G. 2008. Production of polyclonal antisera using recombinant coat proteins of Grapevine leafroll-associated virus 2 and Grapevine virus B. Pesquisa Agropecuaria Brasileira 43 (10), 1405-1411. RIGOTTI. S., BITTERLIN, W. & GUGERLI, P. 2006. Production of monoclonal antibodies for the serological identification and reliable detection of the seventh grapevine leafroll associated virus (GLRaV- 7) in grapevine. Extended abstracts 15 th Meeting of ICVG, Stellenbosch, South Africa, 200-201. ROSCIGLIONE, B. & GUGERLI, P. 1986. Maladies de l'enroulement et du bois strié de la vigne: analyse microscopique et sérologique. (Leafroll and stem pitting of grapevine: microscopical and serological analysis). Revue Suisse de Viticulture, Arboriculture et Horticulture 18, 207-211. SALDARELLI, P. CORNUET, P., VIGNE, E., TALAS, F., BRONNENKANT, I., DRIDI, A.M., ANDRET-LINK, P., BOSCIA, D., GUGERLI, P., FUCHS, M. & MARTELLI, G.P. 2006. Partial characterization of two divergent variants of grapevine leafroll-associated virus 4. Journal of Plant Pathology 88 (2), 203- 214, 2006. Progrès Agricole et Viticole, 2009, Hors Série – Extended abstracts 16 th Meeting of ICVG, Dijon, France, 31 Aug – 4 Sept 2009
Page 3 and 4: 16th Meeting of the International C
Page 5 and 6: AVANT-PROPOS Nous sommes très heur
Page 7 and 8: Content - Sommaire Page Partners an
Page 9 and 10: Session IV - Epidemiology - Survey
Page 11 and 12: Poster 51 Poster 52 Poster 53 Poste
Page 13 and 14: Identification and distribution of
Page 15 and 16: — 15 — GRAPEVINE VIROLOGY HIGHL
Page 17 and 18: — 17 — were found, in that a va
Page 19 and 20: — 19 — Likewise, an antibody bi
Page 21 and 22: — 21 — LITERATURE ABOU GHANEM-S
Page 23 and 24: — 23 — ORECCHIA, M., NOELKE, G.
Page 25: — 25 — immunoassays, either ELI
Page 29 and 30: — 29 — NEW DEVELOPMENTS IN UNDE
Page 31 and 32: — 31 — LIU, Y.-P., PEREMYSLOV,
Page 33 and 34: — 33 — RESULTS AND DISCUSSION O
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Page 37 and 38: — 37 — viruses multiple primers
Page 39 and 40: — 39 — glass planar microarrays
Page 41 and 42: — 41 — Fig. 2. Part of a Micros
Page 43 and 44: — 43 — Complete sequencing of G
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Page 47 and 48: — 47 — result suggests that the
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Page 51 and 52: — 51 — acquisition and analysis
Page 53 and 54: — 53 — identity at the protein
Page 55 and 56: — 55 — The sequences obtained w
Page 57 and 58: — 57 — MULLIS, K. & FALOONA, F.
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Page 63 and 64: — 63 — Madeira Islands. Eight a
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Page 69 and 70: — 69 — IDENTIFICATION OF PLASMO
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Page 73 and 74: — 73 — RECOMBINATION EVENTS IN
Page 75 and 76: — 75 — THE SPECIFICITY OF GFLV
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— 77 — PARTIAL RNA-2 SEQUENCE O
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— 79 — CHARACTERIZATION OF RASP
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— 81 — ISOLATION OF MOVEMENT PR
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— 83 — REAL-TIME PCR OF GRAPEVI
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— 85 — FIRST RECORDS OF ARABIS
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— 87 — primers allow us to quan
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— 89 — transgenic grape lines,
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— 91 — highest values were obse
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— 93 — Imura et al., 2008, Loud
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— 95 — GFLV-F13. Low levels of
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— 97 — This is probably due to
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— 99 — variation was distribute
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— 101 — Figure 1. Proportion of
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— 103 — were produced. The prod
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— 105 — 2007 positive results w
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— 107 — dehydrogenase (GAPDH) t
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— 109 — detected, either as sin
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— 111 — basal leaf tissue inclu
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— 113 — GLRaV-3 incidence cover
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— 115 — Figure 2. Frequency of
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— 117 — Ljutun, Mladenka, Nincu
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— 119 — ELISA reagent used in t
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— 121 — Due the late season the
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— 123 — RT-PCR & PCR: Primers f
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— 125 — Table 2. Sanitary statu
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— 127 — SANITARY STATUS OF AUTO
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— 129 — THE VIRUS STATUS OF GRA
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— 131 — PRESENCE OF GLRAV 1, 2,
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