Introduction to Genome Annotation - CPGR

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Structural Annotation: Graphic Viewer Annotation Station Sequence Database Hits Top: Protein matches Bottom: EST matches Not shown graphically: gene name, nucleotide and protein sequence, MW, pI, organellar targeting sequence, membrane spanning regions, other domains. Gene Predictions Annotated Gene Top: editing panel Bottom: final curation Splice site predictions: red: acceptor sites blue: donor sites Screenshot of a component within Neomorphic’s annotation station: www.neomorphic.com
Features Typically Resolved During Manual Annotation • incorrect exon boundaries • merged, split, missing genes • missing untranslated regions (UTRs) • missing alternative splicing isoform annotations • degenerate transposons annotated as proteincoding genes
Page 1 and 2: Introduction to Genome Annotation A
Page 3 and 4: Annotation pipeline Generation of O
Page 5 and 6: Prokaryotic Gene Structure and Tran
Page 7 and 8: Structural Annotation: Finding the
Page 9 and 10: Candidate Genes 6-frame ORF map Sto
Page 11 and 12: Eukaryotic Gene Finding Identifying
Page 13 and 14: Experimental Evidence DNA sequence
Page 15 and 16: Functional Assignments Name Descrip
Page 17 and 18: Sequence Alignments Compare sequenc
Page 19 and 20: Functional Annotation: Gene Product
Page 21: Automated Annotation is Not a Solve
Page 25: Caveats of Genome Annotation -Great

Introduction to Genome Annotation - CPGR

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