What is cell fractionation

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1 . Homogenization First the cells must be broken open. A variety of different methods are available; the method chosen depends on the type of experiment and the type of sample (bacterial culture or mammalian tissue sample, for example) Detergents like SDS or Triton X disrupt the cell membrane so the contents can flow out. Subjecting the cells to ultrasound waves or sonicating them will also break them open, as will agitation in the presence of metal or glass beads. Blenders may work with tissue samples but will not work with bacteria or other microorganisms.
Homogenization or Cell Disruption ‣Chemical : alkali, organic solvents, detergents ‣Enzymatic : lysozyme , chitinase ‣Physical : osmotic shock, freeze/thaw ‣Mechanical : sonication , homogenization, French press
Page 2 and 3: Historical Introduction
Page 4 and 5: The electronmicroscope revealed muc
Page 6 and 7: • Cell fractionation is where a c
Page 8 and 9: Cell fractionation methods Involve
Page 12 and 13: Chemical Disruption • Detergents
Page 14 and 15: Homogenization • Cells are placed
Page 16 and 17: Differential Centrifugation Differe
Page 18 and 19: • These are both test tubes attac
Page 20 and 21: • The preparative ultracentrifuge
Page 22 and 23: Tow types of centrifuge are there F
Page 24 and 25: Method of Differential Centrifugati
Page 26 and 27: • 5. Centrifuge at medium speeds
Page 28 and 29: • 7 Centrifuge at very high speed
Page 30 and 31: An illustration of the sucrose grad
Page 32 and 33: 5 . Analysis of fractions Analysis
Page 34 and 35: Investigating Cell Function • Dif
Page 37: Represented by

What is cell fractionation

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