What is cell fractionation

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Cell fractionation methods Involve the homogenization or destruction of cell boundaries by different mechanical or chemical procedures, followed by the separation of the subcellular fractions according to mass, surface, and specific gravity
Steps of subcellular fractionation 1 . Homogenization 2 . Differential centrifugation 3 . Further separation and purification by density gradient centrifugation 4 . Collection of fractions 5 . Analysis of fractions
Page 2 and 3: Historical Introduction
Page 4 and 5: The electronmicroscope revealed muc
Page 6 and 7: • Cell fractionation is where a c
Page 10 and 11: 1 . Homogenization First the cells
Page 12 and 13: Chemical Disruption • Detergents
Page 14 and 15: Homogenization • Cells are placed
Page 16 and 17: Differential Centrifugation Differe
Page 18 and 19: • These are both test tubes attac
Page 20 and 21: • The preparative ultracentrifuge
Page 22 and 23: Tow types of centrifuge are there F
Page 24 and 25: Method of Differential Centrifugati
Page 26 and 27: • 5. Centrifuge at medium speeds
Page 28 and 29: • 7 Centrifuge at very high speed
Page 30 and 31: An illustration of the sucrose grad
Page 32 and 33: 5 . Analysis of fractions Analysis
Page 34 and 35: Investigating Cell Function • Dif
Page 37: Represented by

What is cell fractionation

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