What is cell fractionation

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Method of Differential Centrifugation: • 1. Cut tissue in an icecold isotonic buffer. It is cold to stop enzyme reactions, isotonic to stop osmosis and a buffer to stop pH changes. • 2. Grind tissue in a blender to break open cells. • 3. Filter to remove insoluble tissue
• 4. Centrifuge filtrate at low speeds ( 1000 X g for 10mins ) • This pellets the nuclei as this is the densest organelle
Page 2 and 3: Historical Introduction
Page 4 and 5: The electronmicroscope revealed muc
Page 6 and 7: • Cell fractionation is where a c
Page 8 and 9: Cell fractionation methods Involve
Page 10 and 11: 1 . Homogenization First the cells
Page 12 and 13: Chemical Disruption • Detergents
Page 14 and 15: Homogenization • Cells are placed
Page 16 and 17: Differential Centrifugation Differe
Page 18 and 19: • These are both test tubes attac
Page 20 and 21: • The preparative ultracentrifuge
Page 22 and 23: Tow types of centrifuge are there F
Page 26 and 27: • 5. Centrifuge at medium speeds
Page 28 and 29: • 7 Centrifuge at very high speed
Page 30 and 31: An illustration of the sucrose grad
Page 32 and 33: 5 . Analysis of fractions Analysis
Page 34 and 35: Investigating Cell Function • Dif
Page 37: Represented by

What is cell fractionation

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