What is cell fractionation

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• The preparative ultracentrifuge. Sample is contained in tubes that are inserted into a ring of cylindrical holes in a metal rotor. Rapid rotation of the rotor generates enormous centrifugal forces, which cause particles in the sample to sediment. The vacuum reduces friction, preventing heating of the rotor and allowing the refrigeration system to maintain the sample at 4°C.
‣When a centrifugal force is applied to an aqueous mixture, components of larger size and density will sediment faster ‣Low speed centrifugation is used to separate intact cells from medium ‣High speed centrifugation can be used to separate subcellular components
Page 2 and 3: Historical Introduction
Page 4 and 5: The electronmicroscope revealed muc
Page 6 and 7: • Cell fractionation is where a c
Page 8 and 9: Cell fractionation methods Involve
Page 10 and 11: 1 . Homogenization First the cells
Page 12 and 13: Chemical Disruption • Detergents
Page 14 and 15: Homogenization • Cells are placed
Page 16 and 17: Differential Centrifugation Differe
Page 18 and 19: • These are both test tubes attac
Page 22 and 23: Tow types of centrifuge are there F
Page 24 and 25: Method of Differential Centrifugati
Page 26 and 27: • 5. Centrifuge at medium speeds
Page 28 and 29: • 7 Centrifuge at very high speed
Page 30 and 31: An illustration of the sucrose grad
Page 32 and 33: 5 . Analysis of fractions Analysis
Page 34 and 35: Investigating Cell Function • Dif
Page 37: Represented by

What is cell fractionation

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