Fc-Fusion Proteins: A Growing Class of Therapeutics - Austropa ...

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How Components Impact on Yield• Expression vector è expression level• Transfection method è copy number• Host cell line è secretion, p-t modifications• Selection method è strength <strong>of</strong> expression• Media è yield, structure• Culture conditions è yield, structureGENETICSPROCESSCONFIDENTIAL
Difficult to Express Protein <strong>Class</strong>esGPCRskinasesion channelsblood plasma proteinsvaccinesAntibodies…CONFIDENTIAL
Page 6 and 7: Design of CD4IgG• Assembles into
Page 8 and 9: 34 Therapeutic monoclonal antibody/
Page 10 and 11: Potential production issues with Fc
Page 12 and 13: Presentations• Engineering a CHO
Page 14 and 15: Cell Line Development Process Desir
Page 18 and 19: CONFIDENTIAL10VHc250 AntibodyCombin
Page 20 and 21: Approaches to Debottleneck Protein
Page 22 and 23: Inefficient Processing and Secretio
Page 24 and 25: Chaperones operating in the ER…So
Page 26 and 27: Metabolically Engineered CellsLow-P
Page 28 and 29: Volumetric Productivity in Fed Batc
Page 30 and 31: ConclusionsBottlenecks are specific
Page 32 and 33: Bioprocessing Challenges ofFusion P
Page 34 and 35: First Example: Fc-Fusion + mFactor
Page 36 and 37: Temper Expectations on Expression R
Page 38 and 39: Fusion Proteins Tend to AggregateCo
Page 40 and 41: Other Strategies for Protein A Elut
Page 42 and 43: Removal of Aggregates -- SEC• Whe
Page 44 and 45: Even Nanofiltration Can be Problema

Fc-Fusion Proteins: A Growing Class of Therapeutics - Austropa ...

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