Fc-Fusion Proteins: A Growing Class of Therapeutics - Austropa ...

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Design of CD4IgG• Assembles into ahomodimer• Retains fidelity ofcleavage site by papainin hinge• Binds C1q• Binds Protein A• Is transported acrossplacenta Chamow et al., Biochemistry 29, 9885-9891 (1990)6
Structural variety of Fc-fusion proteins2008--At least 40 Fc-fusion cytokines describedJazayeri and Carroll, Biodrugs 22, 11-26 (2008)7
Page 8 and 9: 34 Therapeutic monoclonal antibody/
Page 10 and 11: Potential production issues with Fc
Page 12 and 13: Presentations• Engineering a CHO
Page 14 and 15: Cell Line Development Process Desir
Page 16 and 17: How Components Impact on Yield• E
Page 18 and 19: CONFIDENTIAL10VHc250 AntibodyCombin
Page 20 and 21: Approaches to Debottleneck Protein
Page 22 and 23: Inefficient Processing and Secretio
Page 24 and 25: Chaperones operating in the ER…So
Page 26 and 27: Metabolically Engineered CellsLow-P
Page 28 and 29: Volumetric Productivity in Fed Batc
Page 30 and 31: ConclusionsBottlenecks are specific
Page 32 and 33: Bioprocessing Challenges ofFusion P
Page 34 and 35: First Example: Fc-Fusion + mFactor
Page 36 and 37: Temper Expectations on Expression R
Page 38 and 39: Fusion Proteins Tend to AggregateCo
Page 40 and 41: Other Strategies for Protein A Elut
Page 42 and 43: Removal of Aggregates -- SEC• Whe
Page 44 and 45: Even Nanofiltration Can be Problema

Fc-Fusion Proteins: A Growing Class of Therapeutics - Austropa ...

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