Core-Shell

Examples of transferIsocratic separationHPLC12311/3 of analysis time0 2 4 6 8 10 12 14 16 18 20 22 24Retention time/minN(4)=20,2871SunShell C18, 2.6 mm 100 x 4.6 mm452233ACE C18, 5 mm 250 x 4.6 mm0 1 2 3 4 5 6Retention time/min44N(4)=19,3135N(4)=20,2875Column:ACE C18, 5 mm 250 x 4.6 mmSunShell C18, 2.6 mm 100 x 4.6 mmMobile phase:CH 3 CN/20mM Phosphoric acid = 45/55Flow rate: 1.0 mL/min,1.8 mL/min at the lowest chromatogramTemperature: 25 ºCPressure: 9.5 MPa for Brand F C18 5 mm13.4 MPa for SunShell C18 2.6 mmDetection: UV@230 nmSample: 1 = Benzydamine2 = Ketoprofen3 = Naproxen4 = Indomethacin5 = IbuprofenUHPLC1231.0 mL/min4N(4)=24,1245HPLC: Hitachi LaChrom ELITE (using 0.25 mm i.d. tubing)UHPLC: Jasco X-LC123451.8 mL/minN(4)=22,424Analysisi time decreases to 1/6 to comparewith a 5mm column sized 250x4.6mm.0 1 2 3 4 5 6Retention time/minGradient separationHPLC140 2 4 6 8 10 12 14 16 18 20 22 24Retention time/min12Sunniest C18, 5 mm 150 x 4.6 mm340 1 2 3 4 5 6Retention time/min152SunShell C18, 2.6 mm 75 x 4.6 mm6728 93341/2 of Analysis time5656778 98 97 8 9Column:Sunniest C18, 5 mm 150 x 4.6 mmSunShell C18, 2.6 mm 75 x 4.6 mmMobile phase:A) 0.1% Phosphoric acidB) CH 3 CNGradient program for Sunniest C18for SunShell C18Time 0 min 15 min 20 min%B 2% 25% 25%Time 0 min 7.5 min 10 min%B 2% 25% 25%Flow rate: 1.0 mL/min,Temperature: 25 ºCDetection: UV@250 nmSample: Oolong tea1 = Gallocatechin, 2 = Epigallocatechin,3 = Catechin, 4 = Caffeine, 5 = Epicatechin,6 = Epigallocatechin gallate, 7 = Gallocatechingallate, 8 = Epicatechin gallate, 9 = Catechin gallateUHPLC120 1 2 3 4 5 6Retention time/min345678 9HPLC: Hitachi LaChrom ELITE (using 0.25 mm i.d. tubing)UHPLC: Jasco X-LCThere are difference of system time lagbetween HPLC and UHPLC. UHPLC has muchless than system time lag than HPLC because ofhigh pressure gradient system for UHPLC andlow pressure gradient system for HPLC.Biotech ABE-mail: info@biotech.seURL: www.biotech.seChromaNik Technologies Inc.URL: http://chromanik.co.jp