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Efficiency of Sun<strong>Shell</strong> C18UHPLCColumn: Sun<strong>Shell</strong> C18, 50 x 2.1 mm3Plate(3)=10,3001240 1 2 3 4 5Retention time/minColumn: Sun<strong>Shell</strong> C18, 100 x 2.1 mm31 2Plate(3)=21,3004Column: Sun<strong>Shell</strong> C18, 2.6 µm 50 x 2.1 mmMobile phase: CH 3 CN/H 2 O=60/40Flow rate: 0.3 mL/minPressure: 7 MPaTemperature: 23 ºCSample: 1 = Uracil2 = Toluene3 = Acenaphthene4 = ButylbenzeneColumn: Sun<strong>Shell</strong> C18, 2.6 µm 100 x 2.1 mmMobile phase: CH 3 CN/H 2 O=60/40Flow rate: 0.3 mL/minPressure: 12.5 MPaTemperature: 25 ºC0 2 4 6 8Retention time/minColumn: Sun<strong>Shell</strong> C18,150 x 4.6 mmEfficiency=253,000 plate/m1120 2 4 6 8 10Retention time/minColumn: Sun<strong>Shell</strong> C18, 150 x 4.6 mm32Plate(3)=38,00034Plate(3)=31,0004HPLC80% efficiency tocompare with UHPLCColumn: Sun<strong>Shell</strong> C18, 2.6 µm 150 x 4.6 mmSun<strong>Shell</strong> C18, 2.6 µm 100 x 4.6 mmMobile phase: CH 3 CN/H 2 O=70/30Flow rate: 1.0 mL/minPressure: 14.5MPa(UHPLC),13.5 MPa(HPLC) for 150 mm9.5MPa(HPLC) for 100 mmTemperature: 25 ºCSample: 1 = Uracil2 = Toluene3 = Acenaphthene4 = Butylbenzene1Column: Sun<strong>Shell</strong> C18, 100 x 4.6 mm23Plate(3)=20,00040 2 4 6 8 10Retention time/min0 2 4 6 8 10Retention time/minLaChrom ELITEThe same efficiency as5 µm, 250 x 4.6 mmSaving 60% foranalytical time andconsumption of solventWebsite : www.chromtech.net.au E-mail : info@chromtech.net.au TelNo : 03 9762 2034 . . . in AUSTRALIA

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