Core-Shell

Particle size(µm)Core shell silicaPore diameter(nm)Specific surfacearea (m 2 /g)SunShell の 基 本 特 性SunShell C8, PFP(Pentafluorophenyl)PFP◆ Characteristics of SunShellCarbon content(%)Bonded phaseBonded phaseMaximum operatingpressureAvailable pHrangeSunShell C18 2.6 9 150 7 C18 60 MPa or 8,570 psi 1.5 - 10SunShell C8 2.6 9 150 4.5 C8 60 MPa or 8,570 psi 1.5 - 9SunShell PFP 2.6 9 150 4.5 Pentafluorophenyl 60 MPa or 8,570 psi 2 - 8◆ Comparison of standard samples112,32356447756SunShell PFPSunShell C8Column:SunShell PFP, 2.6 µm 150 x 4.6 mmSunShell C8, 2.6 µm 150 x 4.6 mmSunShell C18, 2.6 µm 150 x 4.6 mmMobile phase: CH 3 OH/H 2 O=75/25Flow rate: 1.0 mL/minTemperature: 40 ºCSample: 1 = Uracil, 2 = Caffeine, 3 = Phenol, 4 = Butylbenzene5 = o-Terphenyl, 6 = Amylbenzene, 7 = Triphenylene123SunShell C184567Hydrogen bonding(Caffeine/Phenol)Hydrophobicity(Amylbenzene/Butylbenzene)Steric selectivity(Triphenylene/o-Terphenyl)SunShell PFP 1.00 1.31 2.38SunShell C8 0.32 1.46 1.080 2 4 6 8 10 12 14 16 18 20 22 24Retention time/minSunShell C18 0.39 1.60 1.46Retention of standard samples was compared for three kinds of phases such as C18, C8 and PFP. C18 showed the highesthydrophobicity and PFP showed both the highest steric selectivity and the highest hydrogen bonding. The feature of PFP phase isto have hydrogen bonding, dipole-dipole interaction, aromatic and pi-pi interactions and hydrophobicity, which causes a differentselectivity from a C18 phase.Separation of amitriptyline using C8 Separation of xanthines Separation of isomers of xylene34SunShellC181 234WaterSunShell C1812312SunShellPFP1 2 34Water30 1 2 3 4 5Retention time/minSunShell C8, 2.6 µm 150 x 4.6 mmMobile phase:CH 3 CN/20mM phosphate buffer H7.0=60/40Flow rate: 1.0 mL/minTemperature: 40 ºCDetection: UV@250nmSample: 1 = Uracil2 = Propranolol3 = Nortriptyline4 = AmitriptylineSunShellPFPSunShellPFP1 2120mMphosphatebuffer pH750mMphosphatebuffer pH70 1 2 3 4 5Retention time/minSunShell C18, PFP 2.6 µm 150 x 2.1 mmMobile phase: CH 3 OH/water or buffer=30/70Flow rate: 0.3 mL/minTemperature: 25 ºCDetection: UV@250nmSample: 1 = Theobromine2 = Theophyline3 = Caffeine4 = Phenol42 343SunShell PFP0 1 2 3 4 5Retention time/minSunShell C18, PFP 2.6 µm 150 x 2.1 mmMobile phase:CH 3 OH/water=75:25 for SunShell C18CH 3 OH/water=60:40 for SunShell PFPFlow rate: 0.3 mL/minTemperature: 25 ºCDetection: UV@250nmSample: 1 = o-Xylene2 = m-Xylene3 = p-Xylene12SunShell C8 showed a sharp peak foramitriptyline a well as SunShell C18.SunShell PFP can retain xanthines more thanSunShell C18. The higher the concentration ofSunShell PFP showed the different selectivityfrom SunShell C18.Website : www.chromtech.net.au buffer, E-mail the : longer info@chromtech.net.au the retention time. TelNo : 03 9762 2034 . . . in AUSTRALIA